Construct: sgRNA BRDN0001145056
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGATTATACTCTTACACTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R1 (5295)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77091
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
68293195 |
+ |
PIK3R1 |
NNNATTATACTCTTACACTA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
19742604 |
- |
SLC24A2 |
NNNATTATACACTTAAACTA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000017.11 |
17 |
63190372 |
- |
TANC2 |
NNNATAATACTCTTACATTA |
NGG |
2 |
0.5571 |
Tier II |
| 4 |
NC_000011.10 |
11 |
93834842 |
- |
VSTM5 |
NNNTTTATACTATTACACTA |
NGG |
2 |
0.4545 |
Tier II |
| 5 |
NC_000003.12 |
3 |
115023611 |
+ |
ZBTB20 |
NNNAATAAACTCTTACACTA |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000018.10 |
18 |
37227224 |
- |
KIAA1328 |
NNNATTATACTTTTACATTA |
NGG |
2 |
0.3462 |
Tier II |
| 7 |
NC_000011.10 |
11 |
58137543 |
- |
OR9Q1 |
NNNATTATCCTCTTACACTT |
NGG |
2 |
0.3429 |
Tier II |
| 8 |
NC_000010.11 |
10 |
85953013 |
- |
GRID1 |
NNNATTATACTCTTACAATA |
NAG |
2 |
0.1396 |
Tier II |
| 9 |
NC_000012.12 |
12 |
64302819 |
+ |
C12orf56 |
NNNATTATATTCTTACAGTA |
NGG |
2 |
0.1255 |
Tier II |
| 10 |
NC_000003.12 |
3 |
160400522 |
+ |
SMC4 |
NNNATTATACGCTTATACTA |
NGG |
2 |
0.1231 |
Tier II |
| 11 |
NC_000020.11 |
20 |
59931552 |
+ |
SYCP2 |
NNNTTTATACTCTTACCCTA |
NGG |
2 |
0.1123 |
Tier II |
| 12 |
NC_000008.11 |
8 |
112518579 |
+ |
CSMD3 |
NNNATTATAGTCTTCCACTA |
NGG |
2 |
0.1061 |
Tier II |
| 13 |
NC_000008.11 |
8 |
76188204 |
+ |
LOC102724858 |
NNNATTATAGTTTTACACTA |
NGG |
2 |
0.2094 |
Tier III |
| 14 |
NC_000003.12 |
3 |
160400522 |
+ |
TRIM59-IFT80 |
NNNATTATACGCTTATACTA |
NGG |
2 |
0.1231 |
Tier III |
| 15 |
NC_000007.14 |
7 |
41108191 |
+ |
LINC01449 |
NNNATTATTCTCTTTCACTA |
NGG |
2 |
0.12 |
Tier III |
| 16 |
NC_000014.9 |
14 |
41132481 |
- |
LINC02315 |
NNNATTTTACTCTTACACTA |
NAG |
2 |
0.1134 |
Tier III |
| 17 |
NC_000009.12 |
9 |
36305671 |
+ |
LOC102724322 |
NNNTTTATACTCTTACACTA |
NGT |
2 |
0.0103 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
101689404 |
- |
Pik3r1 |
NNNATTACACTCTTACACTA |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
29202927 |
- |
Ppp4r3b |
NNNATTATACTCTTAAATTA |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000070.6 |
4 |
36768355 |
- |
Lingo2 |
NNNATTATACTCTTAAACTT |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000075.6 |
9 |
90093307 |
- |
Morf4l1 |
NNNATTATACTCTTGCACTG |
NGG |
2 |
0.4971 |
Tier II |
| 5 |
NC_000084.6 |
18 |
71737665 |
- |
Dcc |
NNNTTTATACTCATACACTA |
NGG |
2 |
0.4406 |
Tier II |
| 6 |
NC_000077.6 |
11 |
51736244 |
+ |
Sec24a |
NNNATTCTATTCTTACACTA |
NGG |
2 |
0.4118 |
Tier II |
| 7 |
NC_000078.6 |
12 |
31906708 |
- |
Cog5 |
NNNAATAAACTCTTACACTA |
NGG |
2 |
0.4 |
Tier II |
| 8 |
NC_000082.6 |
16 |
32447288 |
- |
Pcyt1a |
NNNATTACACTCTTACACCA |
NGG |
2 |
0.2759 |
Tier II |
| 9 |
NC_000077.6 |
11 |
22970105 |
- |
Commd1 |
NNNATTTTACTCTTACAATA |
NGG |
2 |
0.2356 |
Tier II |
| 10 |
NC_000078.6 |
12 |
41449371 |
+ |
Immp2l |
NNNATTATACTCTCACATTA |
NGG |
2 |
0.1837 |
Tier II |
| 11 |
NC_000082.6 |
16 |
23114429 |
- |
Rfc4 |
NNNAATATACTCTTACACTA |
NTG |
2 |
0.0195 |
Tier II |
| 12 |
NC_000068.7 |
2 |
143767422 |
+ |
Pcsk2 |
NNNATTATACTATTACACTA |
NGT |
2 |
0.0115 |
Tier II |
| 13 |
NC_000069.6 |
3 |
84930753 |
+ |
Fbxw7 |
NNNATTATACTCTTACACTC |
NTG |
2 |
0.0089 |
Tier II |
| 14 |
NC_000069.6 |
3 |
117785718 |
- |
Snx7 |
NNNATTATACTCTTACTCTA |
NTG |
2 |
0.0052 |
Tier II |
| 15 |
NC_000078.6 |
12 |
113579345 |
- |
Igh |
NNNATTATACATTTACACTA |
NGG |
2 |
0.4038 |
Tier III |
| 16 |
NC_000077.6 |
11 |
63114698 |
+ |
Gm36926 |
NNNATTATACTCTTACAATA |
NAG |
2 |
0.1396 |
Tier III |
| 17 |
NC_000068.7 |
2 |
143767422 |
+ |
Pcsk2os2 |
NNNATTATACTATTACACTA |
NGT |
2 |
0.0115 |
Tier III |
Other clones with same target sequence:
(none)
