Construct: sgRNA BRDN0001145059

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGCTCCCAGCAAAGCATCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK15 (65061)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76483

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 201835718 - CDK15 NNNTCCCAGCAAAGCATCAG NGG 0 1.0 Tier I
2 NC_000013.11 13 112876541 - ATP11A NNNTCCCAGTAAAGCAGCAG NGG 2 0.1107 Tier I
3 NC_000017.11 17 21700089 + KCNJ18 NNNTCCCAGCACAGCCTCAG NGG 2 0.0 Tier I
4 NC_000017.11 17 78702269 - CYTH1 NNNTCCCAGCAAAGCATCAT NGG 1 0.7 Tier II
5 NC_000001.11 1 177019004 + ASTN1 NNNTAACAGCAAAGCATCAG NGG 2 0.5306 Tier II
6 NC_000019.10 19 44636625 + IGSF23 NNNTGCAAGCAAAGCATCAG NGG 2 0.45 Tier II
7 NC_000019.10 19 368431 - SPMAP2 NNNTTCCAGCAAAGCATAAG NGG 2 0.3427 Tier II
8 NC_000012.12 12 69646655 + BEST3 NNNTCCCTGCAGAGCATCAG NGG 2 0.3095 Tier II
9 NC_000001.11 1 111497617 + TMIGD3 NNNTCCCAGCAAGCCATCAG NGG 2 0.2795 Tier II
10 NC_000004.12 4 84959778 + WDFY3 NNNTCCCAGCAAAAAATCAG NGG 2 0.1667 Tier II
11 NC_000010.11 10 1333269 - ADARB2 NNNTCCCAGCAAAGCATCAC NAG 2 0.1111 Tier II
12 NC_000015.10 15 27375446 + GABRG3 NNNTCCCAGCAAAGCATCAG NCG 1 0.1071 Tier II
13 NC_000013.11 13 110538762 + RAB20 NNNTCCCAGCAAGGCAGCAG NGG 2 0.0767 Tier II
14 NC_000012.12 12 99558508 + ANKS1B NNNTGCCAGCAAAGCAGCAG NGG 2 0.0706 Tier II
15 NC_000012.12 12 117237046 - NOS1 NNNTCCCAGCAAAGCATCAA NGA 2 0.0651 Tier II
16 NC_000009.12 9 77432251 + GNA14 NNNTCCCAGCACCGCATCAG NGG 2 0.0554 Tier II
17 NC_000011.10 11 47681911 - AGBL2 NNNTCCAAGCAAAGCATCAG NGA 2 0.0521 Tier II
18 NC_000021.9 21 21344279 - NCAM2 NNNACCCAGCAAAGTATCAG NGG 2 0.0317 Tier II
19 NC_000001.11 1 35553344 + KIAA0319L NNNTCCCAGCAAAGCATCAT NTG 2 0.0273 Tier II
20 NC_000001.11 1 185221695 - SWT1 NNNTCCCAGAAAAGCATCAG NGC 2 0.0193 Tier II
21 NC_000016.10 16 5450722 - RBFOX1 NNNTCCCAGCAAGGCATCAG NGC 2 0.0145 Tier II
22 NC_000010.11 10 24106203 - KIAA1217 NNNTCCCAGCCAAGCATCAG NGT 2 0.0065 Tier II
23 NC_000015.10 15 26565541 + GABRB3 NNNTCCCAGCAAAGCCACAG NGG 2 0.0 Tier II
24 NC_000003.12 3 141095470 - SPSB4 NNNTCCCAGCAAAGTCTCAG NGG 2 0.0 Tier II
25 NC_000017.11 17 21412626 + KCNJ12 NNNTCCCAGCACAGCCTCAG NGG 2 0.0 Tier II
26 NC_000004.12 4 122673376 + IL21-AS1 NNNTCTCAGCAAAGCAACAG NGG 2 0.4952 Tier III
27 NC_000020.11 20 46740259 - LOC124904918 NNNTCCCAGCGAAACATCAG NGG 2 0.4875 Tier III
28 NC_000012.12 12 69646655 + LOC105369823 NNNTCCCTGCAGAGCATCAG NGG 2 0.3095 Tier III
29 NC_000006.12 6 139443361 + LOC107986651 NNNTCCAAGCATAGCATCAG NGG 2 0.25 Tier III
30 NC_000007.14 7 92851928 + CDK6-AS1 NNNTCCCAGCAATGAATCAG NGG 2 0.0667 Tier III
31 NC_000002.12 2 71712616 - LOC124907827 NNNTCCCAGCAAAGCTTCAG NGC 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 177860019 - Catspere1 NNNTCCCAGCTAAGCATCAA NGG 2 0.2885 Tier I
2 NC_000067.6 1 178098565 - Catspere2 NNNTCCCAGCTAAGCATCAA NGG 2 0.2885 Tier I
3 NC_000067.6 1 59289734 - Cdk15 NNNTCCCAGCAAAGCGTCAG NTG 2 0.0075 Tier I
4 NC_000076.6 10 60831121 + Unc5b NNNTCCCAGCAAAGCAGCAG NGC 2 0.0026 Tier I
5 NC_000069.6 3 99294696 - Tbx15 NNNTCTCAGTAAAGCATCAG NGG 2 0.8739 Tier II
6 NC_000067.6 1 21524489 - Kcnq5 NNNTCCCAGTAAAACATCAG NGG 2 0.7059 Tier II
7 NC_000071.6 5 74450794 + Scfd2 NNNTCCCAGAAGAGCATCAG NGG 2 0.6259 Tier II
8 NC_000072.6 6 115610878 + Mkrn2 NNNGCCCAGCAAAGCATCAG NGG 1 0.625 Tier II
9 NC_000072.6 6 121401200 + Iqsec3 NNNTCCCAGCGGAGCATCAG NGG 2 0.4694 Tier II
10 NC_000084.6 18 63293077 - Piezo2 NNNCCCCAGCAAAGCATCAT NGG 2 0.4529 Tier II
11 NC_000073.6 7 143592173 + Cars NNNTCCCATCAAAGCATTAG NGG 2 0.3429 Tier II
12 NC_000081.6 15 96266260 - Gm38469 NNNTCCCAGCACAGCATCAG NGG 1 0.2632 Tier II
13 NC_000067.6 1 164158973 + F5 NNNTCCCATCAAACCATCAG NGG 2 0.2286 Tier II
14 NC_000074.6 8 123256273 - Zfp276 NNNACCCTGCAAAGCATCAG NGG 2 0.2041 Tier II
15 NC_000080.6 14 60037829 + Atp8a2 NNNTCTCAGCAAAGCATCCG NGG 2 0.1921 Tier II
16 NC_000076.6 10 108214158 - Ppp1r12a NNNTCCCAGCACAGCATCAT NGG 2 0.1842 Tier II
17 NC_000075.6 9 25487893 - Eepd1 NNNTCCCATCAAAGCAGCAG NGG 2 0.0627 Tier II
18 NC_000076.6 10 52069532 + Ros1 NNNTCCCAGCAAAACATCAG NGA 2 0.0521 Tier II
19 NC_000070.6 4 155872751 - Ints11 NNNCCCCAGCAAAGGATCAG NGG 2 0.0324 Tier II
20 NC_000079.6 13 30694773 + Dusp22 NNNTCCCAGCAAAGCATGAG NTG 2 0.0052 Tier II
21 NC_000072.6 6 39422800 - Mkrn1 NNNTCCCAGCAAAGCAGCAG NGC 2 0.0026 Tier II
22 NC_000078.6 12 13381143 + Nbas NNNTCACAGCAAAGCCTCAG NGG 2 0.0 Tier II
23 NC_000069.6 3 55707070 - Nbea NNNTCCCAGCACAGCTTCAG NGG 2 0.0 Tier II
24 NC_000068.7 2 168361290 + Gm33262 NNNTCCCAGCAAGCCATCAG NGG 2 0.2795 Tier III
25 NC_000079.6 13 33434333 - Gm19155 NNNTCCCAGCAAAGCATCAG NAG 1 0.2593 Tier III
26 NC_000078.6 12 8451037 - Gm51967 NNNTCCCAGCAGAGGATCAG NGG 2 0.0361 Tier III
Download CSV

Other clones with same target sequence:

(none)

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