Construct: sgRNA BRDN0001145082
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGATGGCCTGGACAACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MTOR (2475)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76782
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
11228787 |
+ |
MTOR |
NNNGATGGCCTGGACAACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
200891628 |
+ |
INAVA |
NNNGATGGCCTGGACAAACA |
NGT |
2 |
0.0087 |
Tier I |
| 3 |
NC_000003.12 |
3 |
129530900 |
- |
RHO |
NNNGATGGCCAGGACCACCA |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000003.12 |
3 |
129263911 |
- |
COPG1 |
NNNGATGGCCTGGACAACCA |
NCA |
2 |
0.0 |
Tier I |
| 5 |
NC_000002.12 |
2 |
68710996 |
- |
ARHGAP25 |
NNNGATGACCTAGACAACCA |
NGG |
2 |
0.9333 |
Tier II |
| 6 |
NC_000015.10 |
15 |
74334907 |
- |
CCDC33 |
NNNGATGGCCAGGACAACCA |
NGG |
1 |
0.75 |
Tier II |
| 7 |
NC_000001.11 |
1 |
162114659 |
- |
NOS1AP |
NNNGAGGGGCTGGACAACCA |
NGG |
2 |
0.3537 |
Tier II |
| 8 |
NC_000020.11 |
20 |
47721865 |
+ |
SULF2 |
NNNGTTGGCCTGGACAATCA |
NGG |
2 |
0.2338 |
Tier II |
| 9 |
NC_000023.11 |
X |
7074350 |
+ |
PUDP |
NNNTATGGCCTGGACAACAA |
NGG |
2 |
0.1558 |
Tier II |
| 10 |
NC_000007.14 |
7 |
98867854 |
+ |
TMEM130 |
NNNGATGGCCAGGACATCCA |
NGG |
2 |
0.1 |
Tier II |
| 11 |
NC_000001.11 |
1 |
53072580 |
+ |
PODN |
NNNGTTGGCCTGGACAACCC |
NGG |
2 |
0.0826 |
Tier II |
| 12 |
NC_000010.11 |
10 |
14575571 |
+ |
FAM107B |
NNNGATGGCCTGGACACCAA |
NGG |
2 |
0.0756 |
Tier II |
| 13 |
NC_000013.11 |
13 |
107260724 |
+ |
NALF1 |
NNNGATGACCTGGACAACCA |
NGA |
2 |
0.0694 |
Tier II |
| 14 |
NC_000003.12 |
3 |
15636707 |
+ |
BTD |
NNNGATGGCCTGGACAGCCA |
NAG |
2 |
0.0458 |
Tier II |
| 15 |
NC_000002.12 |
2 |
170469542 |
+ |
MYO3B |
NNNGATGGCGTGGAGAACCA |
NGG |
2 |
0.0194 |
Tier II |
| 16 |
NC_000001.11 |
1 |
240870989 |
+ |
RGS7 |
NNNGATGGCCTGGGCTACCA |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000002.12 |
2 |
235699053 |
+ |
AGAP1 |
NNNGGTGGCCTGGACCACCA |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000001.11 |
1 |
68033310 |
- |
GNG12-AS1 |
NNNGAAGGCCTGGACAACAA |
NGG |
2 |
0.3714 |
Tier III |
| 19 |
NC_000001.11 |
1 |
162114659 |
- |
LOC105371475 |
NNNGAGGGGCTGGACAACCA |
NGG |
2 |
0.3537 |
Tier III |
| 20 |
NC_000011.10 |
11 |
106375908 |
+ |
LOC105369474 |
NNNGATGTCCTGGACAAACA |
NGG |
2 |
0.3365 |
Tier III |
| 21 |
NC_000016.10 |
16 |
71588002 |
- |
LOC105371334 |
NNNGATGGGCTGGACATCCA |
NGG |
2 |
0.0825 |
Tier III |
| 22 |
NC_000001.11 |
1 |
67864627 |
+ |
GNG12-AS1 |
NNNTATGGCCTGGAAAACCA |
NGG |
2 |
0.0808 |
Tier III |
| 23 |
NC_000008.11 |
8 |
63994065 |
+ |
LINC01414 |
NNNGATGGCCTGTACAACCA |
NAG |
2 |
0.0778 |
Tier III |
| 24 |
NC_000012.12 |
12 |
49912560 |
- |
LINC02395 |
NNNGATGGCCTGGACAAGTA |
NGG |
2 |
0.0615 |
Tier III |
| 25 |
NC_000006.12 |
6 |
18501421 |
+ |
LOC105374955 |
NNNGATGCCCTGGAGAACCA |
NGG |
2 |
0.0308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
3941986 |
- |
Unc93b1 |
NNNGATGGCCTGGAAAACCA |
NGA |
2 |
0.0154 |
Tier I |
| 2 |
NC_000070.6 |
4 |
148472046 |
- |
Mtor |
NNNGATGGCCTGCACCACCA |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000072.6 |
6 |
87897960 |
- |
Copg1 |
NNNGATGGCCTGGACAACCA |
NCA |
2 |
0.0 |
Tier I |
| 4 |
NC_000074.6 |
8 |
81876233 |
- |
Inpp4b |
NNNAATGGCTTGGACAACCA |
NGG |
2 |
0.8471 |
Tier II |
| 5 |
NC_000082.6 |
16 |
5011138 |
+ |
Rogdi |
NNNGATGGCCAGGACAATCA |
NGG |
2 |
0.4821 |
Tier II |
| 6 |
NC_000071.6 |
5 |
31249126 |
+ |
Nrbp1 |
NNNGAGGGCCAGGACAACCA |
NGG |
2 |
0.4286 |
Tier II |
| 7 |
NC_000070.6 |
4 |
124843294 |
+ |
Mtf1 |
NNNGATTTCCTGGACAACCA |
NGG |
2 |
0.3571 |
Tier II |
| 8 |
NC_000076.6 |
10 |
87872112 |
+ |
Igf1 |
NNNGATGTCCTTGACAACCA |
NGG |
2 |
0.2404 |
Tier II |
| 9 |
NC_000080.6 |
14 |
34044811 |
- |
Gm5460 |
NNNGCTGGCCTGGACAACTA |
NGG |
2 |
0.2308 |
Tier II |
| 10 |
NC_000073.6 |
7 |
36735816 |
- |
Tshz3 |
NNNGATAGCCTGGACAACCA |
NGA |
2 |
0.0694 |
Tier II |
| 11 |
NC_000073.6 |
7 |
144372986 |
- |
Shank2 |
NNNGCTGGCCTGGACATCCA |
NGG |
2 |
0.0667 |
Tier II |
| 12 |
NC_000080.6 |
14 |
101933492 |
- |
Lmo7 |
NNNGAAGGCCTGGACAACCA |
NGA |
2 |
0.0602 |
Tier II |
| 13 |
NC_000070.6 |
4 |
107316682 |
+ |
Yipf1 |
NNNGAAGGCCTGGATAACCA |
NGG |
2 |
0.0578 |
Tier II |
| 14 |
NC_000076.6 |
10 |
30780597 |
- |
Ncoa7 |
NNNGAGGGCCTGGAGAACCA |
NGG |
2 |
0.0286 |
Tier II |
| 15 |
NC_000084.6 |
18 |
14832488 |
+ |
Taf4b |
NNNGAGGGCCTGGAGAACCA |
NGG |
2 |
0.0286 |
Tier II |
| 16 |
NC_000086.7 |
X |
101447989 |
+ |
Nono |
NNNGAGGGCCTGGAGAACCA |
NGG |
2 |
0.0286 |
Tier II |
| 17 |
NC_000074.6 |
8 |
118866803 |
- |
Cdh13 |
NNNGATGGCGTGGACAACCA |
NGA |
2 |
0.027 |
Tier II |
| 18 |
NC_000077.6 |
11 |
46615325 |
- |
Dppa1 |
NNNGATAGCCTGGACTACCA |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000073.6 |
7 |
81693193 |
- |
Homer2 |
NNNGATGGCCTGGCCTACCA |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000081.6 |
15 |
40350048 |
- |
Gm41311 |
NNNCAAGGCCTGGACAACCA |
NGG |
2 |
0.4588 |
Tier III |
| 21 |
NC_000078.6 |
12 |
77174257 |
+ |
Gm40446 |
NNNGAGTGCCTGGACAACCA |
NGG |
2 |
0.3265 |
Tier III |
| 22 |
NC_000073.6 |
7 |
144372986 |
- |
Gm14372 |
NNNGCTGGCCTGGACATCCA |
NGG |
2 |
0.0667 |
Tier III |
| 23 |
NC_000073.6 |
7 |
30521245 |
+ |
Arhgap33os |
NNNGATGGTCTGGAGAACCA |
NGG |
2 |
0.0438 |
Tier III |
| 24 |
NC_000077.6 |
11 |
71243340 |
- |
Nlrp1c-ps |
NNNGATGCCCTGGATAACCA |
NGG |
2 |
0.041 |
Tier III |
| 25 |
NC_000076.6 |
10 |
30780597 |
- |
Gm5171 |
NNNGAGGGCCTGGAGAACCA |
NGG |
2 |
0.0286 |
Tier III |
| 26 |
NC_000084.6 |
18 |
14832488 |
+ |
Gm21055 |
NNNGAGGGCCTGGAGAACCA |
NGG |
2 |
0.0286 |
Tier III |
| 27 |
NC_000086.7 |
X |
80533482 |
- |
Gm8806 |
NNNGAGGGCCTGGAGAACCA |
NGG |
2 |
0.0286 |
Tier III |
Other clones with same target sequence:
(none)
