Construct: sgRNA BRDN0001145082

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGTGATGGCCTGGACAACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTOR (2475)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76782

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 11228787 + MTOR NNNGATGGCCTGGACAACCA NGG 0 1.0 Tier I
2 NC_000001.11 1 200891628 + INAVA NNNGATGGCCTGGACAAACA NGT 2 0.0087 Tier I
3 NC_000003.12 3 129530900 - RHO NNNGATGGCCAGGACCACCA NGG 2 0.0 Tier I
4 NC_000003.12 3 129263911 - COPG1 NNNGATGGCCTGGACAACCA NCA 2 0.0 Tier I
5 NC_000002.12 2 68710996 - ARHGAP25 NNNGATGACCTAGACAACCA NGG 2 0.9333 Tier II
6 NC_000015.10 15 74334907 - CCDC33 NNNGATGGCCAGGACAACCA NGG 1 0.75 Tier II
7 NC_000001.11 1 162114659 - NOS1AP NNNGAGGGGCTGGACAACCA NGG 2 0.3537 Tier II
8 NC_000020.11 20 47721865 + SULF2 NNNGTTGGCCTGGACAATCA NGG 2 0.2338 Tier II
9 NC_000023.11 X 7074350 + PUDP NNNTATGGCCTGGACAACAA NGG 2 0.1558 Tier II
10 NC_000007.14 7 98867854 + TMEM130 NNNGATGGCCAGGACATCCA NGG 2 0.1 Tier II
11 NC_000001.11 1 53072580 + PODN NNNGTTGGCCTGGACAACCC NGG 2 0.0826 Tier II
12 NC_000010.11 10 14575571 + FAM107B NNNGATGGCCTGGACACCAA NGG 2 0.0756 Tier II
13 NC_000013.11 13 107260724 + NALF1 NNNGATGACCTGGACAACCA NGA 2 0.0694 Tier II
14 NC_000003.12 3 15636707 + BTD NNNGATGGCCTGGACAGCCA NAG 2 0.0458 Tier II
15 NC_000002.12 2 170469542 + MYO3B NNNGATGGCGTGGAGAACCA NGG 2 0.0194 Tier II
16 NC_000001.11 1 240870989 + RGS7 NNNGATGGCCTGGGCTACCA NGG 2 0.0 Tier II
17 NC_000002.12 2 235699053 + AGAP1 NNNGGTGGCCTGGACCACCA NGG 2 0.0 Tier II
18 NC_000001.11 1 68033310 - GNG12-AS1 NNNGAAGGCCTGGACAACAA NGG 2 0.3714 Tier III
19 NC_000001.11 1 162114659 - LOC105371475 NNNGAGGGGCTGGACAACCA NGG 2 0.3537 Tier III
20 NC_000011.10 11 106375908 + LOC105369474 NNNGATGTCCTGGACAAACA NGG 2 0.3365 Tier III
21 NC_000016.10 16 71588002 - LOC105371334 NNNGATGGGCTGGACATCCA NGG 2 0.0825 Tier III
22 NC_000001.11 1 67864627 + GNG12-AS1 NNNTATGGCCTGGAAAACCA NGG 2 0.0808 Tier III
23 NC_000008.11 8 63994065 + LINC01414 NNNGATGGCCTGTACAACCA NAG 2 0.0778 Tier III
24 NC_000012.12 12 49912560 - LINC02395 NNNGATGGCCTGGACAAGTA NGG 2 0.0615 Tier III
25 NC_000006.12 6 18501421 + LOC105374955 NNNGATGCCCTGGAGAACCA NGG 2 0.0308 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 3941986 - Unc93b1 NNNGATGGCCTGGAAAACCA NGA 2 0.0154 Tier I
2 NC_000070.6 4 148472046 - Mtor NNNGATGGCCTGCACCACCA NGG 2 0.0 Tier I
3 NC_000072.6 6 87897960 - Copg1 NNNGATGGCCTGGACAACCA NCA 2 0.0 Tier I
4 NC_000074.6 8 81876233 - Inpp4b NNNAATGGCTTGGACAACCA NGG 2 0.8471 Tier II
5 NC_000082.6 16 5011138 + Rogdi NNNGATGGCCAGGACAATCA NGG 2 0.4821 Tier II
6 NC_000071.6 5 31249126 + Nrbp1 NNNGAGGGCCAGGACAACCA NGG 2 0.4286 Tier II
7 NC_000070.6 4 124843294 + Mtf1 NNNGATTTCCTGGACAACCA NGG 2 0.3571 Tier II
8 NC_000076.6 10 87872112 + Igf1 NNNGATGTCCTTGACAACCA NGG 2 0.2404 Tier II
9 NC_000080.6 14 34044811 - Gm5460 NNNGCTGGCCTGGACAACTA NGG 2 0.2308 Tier II
10 NC_000073.6 7 36735816 - Tshz3 NNNGATAGCCTGGACAACCA NGA 2 0.0694 Tier II
11 NC_000073.6 7 144372986 - Shank2 NNNGCTGGCCTGGACATCCA NGG 2 0.0667 Tier II
12 NC_000080.6 14 101933492 - Lmo7 NNNGAAGGCCTGGACAACCA NGA 2 0.0602 Tier II
13 NC_000070.6 4 107316682 + Yipf1 NNNGAAGGCCTGGATAACCA NGG 2 0.0578 Tier II
14 NC_000076.6 10 30780597 - Ncoa7 NNNGAGGGCCTGGAGAACCA NGG 2 0.0286 Tier II
15 NC_000084.6 18 14832488 + Taf4b NNNGAGGGCCTGGAGAACCA NGG 2 0.0286 Tier II
16 NC_000086.7 X 101447989 + Nono NNNGAGGGCCTGGAGAACCA NGG 2 0.0286 Tier II
17 NC_000074.6 8 118866803 - Cdh13 NNNGATGGCGTGGACAACCA NGA 2 0.027 Tier II
18 NC_000077.6 11 46615325 - Dppa1 NNNGATAGCCTGGACTACCA NGG 2 0.0 Tier II
19 NC_000073.6 7 81693193 - Homer2 NNNGATGGCCTGGCCTACCA NGG 2 0.0 Tier II
20 NC_000081.6 15 40350048 - Gm41311 NNNCAAGGCCTGGACAACCA NGG 2 0.4588 Tier III
21 NC_000078.6 12 77174257 + Gm40446 NNNGAGTGCCTGGACAACCA NGG 2 0.3265 Tier III
22 NC_000073.6 7 144372986 - Gm14372 NNNGCTGGCCTGGACATCCA NGG 2 0.0667 Tier III
23 NC_000073.6 7 30521245 + Arhgap33os NNNGATGGTCTGGAGAACCA NGG 2 0.0438 Tier III
24 NC_000077.6 11 71243340 - Nlrp1c-ps NNNGATGCCCTGGATAACCA NGG 2 0.041 Tier III
25 NC_000076.6 10 30780597 - Gm5171 NNNGAGGGCCTGGAGAACCA NGG 2 0.0286 Tier III
26 NC_000084.6 18 14832488 + Gm21055 NNNGAGGGCCTGGAGAACCA NGG 2 0.0286 Tier III
27 NC_000086.7 X 80533482 - Gm8806 NNNGAGGGCCTGGAGAACCA NGG 2 0.0286 Tier III
Download CSV

Other clones with same target sequence:

(none)

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