Construct: sgRNA BRDN0001145087
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTATGGAACGTGATACAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRPK1 (6732)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77493
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
35869853 |
- |
SRPK1 |
NNNATGGAACGTGATACAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
123873824 |
- |
FER1L6 |
NNNAAGGAATGTGATACAGA |
NGG |
2 |
0.4706 |
Tier II |
| 3 |
NC_000016.10 |
16 |
81529321 |
+ |
CMIP |
NNNATGGTACATGATACAGA |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000002.12 |
2 |
130366795 |
- |
PTPN18 |
NNNAAGGAACGTGATACAAA |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000001.11 |
1 |
43243447 |
- |
CFAP57 |
NNNATGGGACCTGATACAGA |
NGG |
2 |
0.3143 |
Tier II |
| 6 |
NC_000022.11 |
22 |
43156619 |
+ |
TSPO |
NNNTTGGAACTTGATACAGA |
NGG |
2 |
0.2448 |
Tier II |
| 7 |
NC_000002.12 |
2 |
47007552 |
- |
TTC7A |
NNNATGGAACGTGGCACAGA |
NGG |
2 |
0.1273 |
Tier II |
| 8 |
NC_000006.12 |
6 |
40562112 |
+ |
LRFN2 |
NNNATGGAAAGTGATAGAGA |
NGG |
2 |
0.051 |
Tier II |
| 9 |
NC_000013.11 |
13 |
41587141 |
+ |
VWA8 |
NNNATGGAACGTGATACTGA |
NGC |
2 |
0.0111 |
Tier II |
| 10 |
NC_000010.11 |
10 |
19147743 |
+ |
MALRD1 |
NNNATGGAAGGTGATTCAGA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000001.11 |
1 |
174608662 |
+ |
RABGAP1L |
NNNATGGAATGTGATTCAGA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000019.10 |
19 |
42560343 |
+ |
LIPE-AS1 |
NNNATGGGACGTAATACAGA |
NGG |
2 |
0.6769 |
Tier III |
| 13 |
NC_000005.10 |
5 |
44749371 |
- |
MRPS30-DT |
NNNAGGGAAAGTGATACAGA |
NGG |
2 |
0.5547 |
Tier III |
| 14 |
NC_000001.11 |
1 |
43243447 |
- |
LOC105378685 |
NNNATGGGACCTGATACAGA |
NGG |
2 |
0.3143 |
Tier III |
| 15 |
NC_000001.11 |
1 |
43243447 |
- |
LOC124904164 |
NNNATGGGACCTGATACAGA |
NGG |
2 |
0.3143 |
Tier III |
| 16 |
NC_000023.11 |
X |
22313548 |
+ |
PTCHD1-AS |
NNNATGGAATGTGACACAGA |
NGG |
2 |
0.2567 |
Tier III |
| 17 |
NC_000003.12 |
3 |
86911698 |
- |
LOC105377196 |
NNNCTGGAAGGTGATACAGA |
NGG |
2 |
0.1373 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
9172347 |
+ |
Sntg1 |
NNNATGGAAAATGATACAGA |
NGG |
2 |
0.8667 |
Tier II |
| 2 |
NC_000070.6 |
4 |
121546770 |
+ |
Gm12880 |
NNNATAGAACGTGATACATA |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000076.6 |
10 |
90363323 |
- |
Anks1b |
NNNATGGAATGTGAAACAGA |
NGG |
2 |
0.5449 |
Tier II |
| 4 |
NC_000080.6 |
14 |
34973736 |
- |
Grid1 |
NNNATGGAACATGGTACAGA |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000083.6 |
17 |
43458577 |
+ |
Adgrf5 |
NNNATGTAACGAGATACAGA |
NGG |
2 |
0.4571 |
Tier II |
| 6 |
NC_000073.6 |
7 |
98098774 |
+ |
Myo7a |
NNNATGGAATGTGGTACAGA |
NGG |
2 |
0.4392 |
Tier II |
| 7 |
NC_000084.6 |
18 |
67126583 |
- |
Gnal |
NNNATGGAACGAGATACTGA |
NGG |
2 |
0.4 |
Tier II |
| 8 |
NC_000085.6 |
19 |
34103266 |
- |
Lipm |
NNNATAGAACGTGATACAGA |
NAG |
2 |
0.2593 |
Tier II |
| 9 |
NC_000071.6 |
5 |
143495066 |
+ |
Daglb |
NNNCTGGAACCTGATACAGA |
NGG |
2 |
0.1513 |
Tier II |
| 10 |
NC_000071.6 |
5 |
121455364 |
- |
Tmem116 |
NNNATGGAACGTTATACACA |
NGG |
2 |
0.1345 |
Tier II |
| 11 |
NC_000068.7 |
2 |
10078177 |
+ |
Atp5c1 |
NNNATGGAACGTTATGCAGA |
NGG |
2 |
0.0577 |
Tier II |
| 12 |
NC_000076.6 |
10 |
13393783 |
+ |
Phactr2 |
NNNATGGAATGTGATAGAGA |
NGG |
2 |
0.0554 |
Tier II |
| 13 |
NC_000075.6 |
9 |
40354807 |
+ |
Gramd1b |
NNNATGGAACGTGAGACAGA |
NGG |
1 |
0.05 |
Tier II |
| 14 |
NC_000078.6 |
12 |
7711104 |
- |
Gm32828 |
NNNATGGAACTTGGTACAGA |
NGG |
2 |
0.1795 |
Tier III |
Other clones with same target sequence:
(none)
