Construct: sgRNA BRDN0001145097

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAGGGTCATCACACACAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD2 (914)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75825

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 116760542 + CD2 NNNGGGTCATCACACACAAG NGG 0 1.0 Tier I
2 NC_000016.10 16 1210404 + CACNA1H NNNAGGTCATCACACACAAG NTG 2 0.0351 Tier I
3 NC_000017.11 17 32538937 + MYO1D NNNGGAACATCACACACAAG NGG 2 0.875 Tier II
4 NC_000003.12 3 29696865 + RBMS3 NNNGAGTCATCATACACAAG NGG 2 0.6067 Tier II
5 NC_000002.12 2 171146374 + TLK1 NNNGGGTGCTCACACACAAG NGG 2 0.3673 Tier II
6 NC_000011.10 11 61949878 + BEST1 NNNGGGTCACCACACACAGG NGG 2 0.2 Tier II
7 NC_000017.11 17 66860413 + CACNG5 NNNGGTTCATCACACACAAG NGA 2 0.0463 Tier II
8 NC_000002.12 2 72431823 - EXOC6B NNNGGGTCATCACACACCAG NGC 2 0.0042 Tier II
9 NC_000023.11 X 50583433 - SHROOM4 NNNGGGCCATCACACTCAAG NGG 2 0.0 Tier II
10 NC_000002.12 2 171146374 + LOC124906168 NNNGGGTGCTCACACACAAG NGG 2 0.3673 Tier III
11 NC_000004.12 4 71643691 - LOC105377271 NNNGGGTCATAACACACAAC NGG 2 0.3214 Tier III
12 NC_000001.11 1 238399808 + LOC105373220 NNNGGGTCATCAAACACAAG NGA 2 0.0267 Tier III
13 NC_000007.14 7 136109080 - LOC105375523 NNNGGGTCATCACACACCAG NGC 2 0.0042 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 58702264 - Olfr319 NNNGTGTCTTCACACACAAG NGG 2 0.18 Tier I
2 NC_000079.6 13 81459820 + Adgrv1 NNNGGGTCATCAGACAAAAG NGG 2 0.0636 Tier I
3 NC_000073.6 7 43784891 - Klk10 NNNGTGTCATCACACACCAG NGG 2 0.0571 Tier I
4 NC_000075.6 9 64948265 - Slc24a1 NNNGAGTCATCACACACAAG NTG 2 0.0338 Tier I
5 NC_000071.6 5 32341865 - Plb1 NNNGGGTCATCACTCACATG NGG 2 0.2872 Tier II
6 NC_000077.6 11 121077813 + Sectm1a NNNGTGTCATCACACACAAA NGG 2 0.2813 Tier II
7 NC_000070.6 4 152179018 + Acot7 NNNGGGTCATCTCACACAAT NGG 2 0.2333 Tier II
8 NC_000075.6 9 88770128 - Gm10634 NNNGTGTCATAACACACAAG NGG 2 0.225 Tier II
9 NC_000075.6 9 88918184 + Gm2396 NNNGTGTCATAACACACAAG NGG 2 0.225 Tier II
10 NC_000074.6 8 122330823 + Zfpm1 NNNTGGGCATCACACACAAG NGG 2 0.2139 Tier II
11 NC_000077.6 11 82435286 + Tmem132e NNNGGGTCATCTCACAAAAG NGG 2 0.1556 Tier II
12 NC_000081.6 15 83141919 + Poldip3 NNNGGGTCGTCACAAACAAG NGG 2 0.1481 Tier II
13 NC_000083.6 17 26428684 - Neurl1b NNNGGGTCAGCACACACAAG NAG 2 0.1296 Tier II
14 NC_000075.6 9 49397374 - Drd2 NNNGGGTCCTCACAAACAAG NGG 2 0.127 Tier II
15 NC_000067.6 1 64927906 + Plekhm3 NNNAGGTCATCAGACACAAG NGG 2 0.1227 Tier II
16 NC_000067.6 1 78547875 + Mogat1 NNNGGGTCATCACCCACAGG NGG 2 0.0804 Tier II
17 NC_000073.6 7 50828021 + Nell1 NNNGAGTCATCACACACAAG NGA 2 0.0602 Tier II
18 NC_000068.7 2 15806677 + Malrd1 NNNGGGTTATCACACAGAAG NGG 2 0.0515 Tier II
19 NC_000076.6 10 73327815 + Pcdh15 NNNGGGTCACCACACACAAG NTG 2 0.0208 Tier II
20 NC_000085.6 19 42748225 + Pyroxd2 NNNGGGTCATCACCCACAAG NTG 2 0.0083 Tier II
21 NC_000071.6 5 32341865 - Gm36840 NNNGGGTCATCACTCACATG NGG 2 0.2872 Tier III
22 NC_000071.6 5 147747658 - Gm35541 NNNGTGTCATCATACACAAG NGG 2 0.21 Tier III
23 NC_000068.7 2 144466412 + Gm38402 NNNGGCTCATCAGACACAAG NGG 2 0.093 Tier III
24 NC_000068.7 2 144466412 + Zfp133-ps NNNGGCTCATCAGACACAAG NGG 2 0.093 Tier III
25 NC_000080.6 14 22015189 - Gm41106 NNNGGGTCAGCACACACAAG NGC 2 0.0111 Tier III
26 NC_000085.6 19 42748225 + Gm19437 NNNGGGTCATCACCCACAAG NTG 2 0.0083 Tier III
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Other clones with same target sequence:

(none)