Construct: sgRNA BRDN0001145127
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGAGACAAAGTCATACACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UCK2 (7371)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75765
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
165891299 |
- |
UCK2 |
NNNAGACAAAGTCATACACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
200836881 |
- |
CAMSAP2 |
NNNAGACATAGACATACACG |
NGG |
2 |
0.48 |
Tier II |
| 3 |
NC_000003.12 |
3 |
112177442 |
- |
SLC9C1 |
NNNAGACAAAGTCTTACACG |
NAG |
2 |
0.1383 |
Tier II |
| 4 |
NC_000002.12 |
2 |
24296493 |
+ |
ITSN2 |
NNNAAACAAAGTCATACAGG |
NGG |
2 |
0.1083 |
Tier II |
| 5 |
NC_000007.14 |
7 |
100873381 |
- |
TRIP6 |
NNNTGACAAAGTCATACAGG |
NGG |
2 |
0.0795 |
Tier II |
| 6 |
NC_000001.11 |
1 |
155422063 |
- |
ASH1L |
NNNAGACAAATTCATACAGG |
NGG |
2 |
0.0481 |
Tier II |
| 7 |
NC_000010.11 |
10 |
123941487 |
- |
CPXM2 |
NNNAGACAAAGTCATACAGG |
NGT |
2 |
0.002 |
Tier II |
| 8 |
NC_000014.9 |
14 |
22275476 |
- |
TRA |
NNNAGACAAAGACATACACT |
NGG |
2 |
0.56 |
Tier III |
| 9 |
NC_000022.11 |
22 |
25475660 |
+ |
LOC124905094 |
NNNAGACACAGTCATCCACG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
167236627 |
+ |
Uck2 |
NNNAGACAAAGTCGTACACG |
NGG |
1 |
0.4667 |
Tier I |
| 2 |
NC_000081.6 |
15 |
103231687 |
+ |
Cbx5 |
NNNAGACAAAGACATACACA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000074.6 |
8 |
11331602 |
- |
Col4a2 |
NNNAAACAAAGTCATACACT |
NGG |
2 |
0.6067 |
Tier II |
| 4 |
NC_000068.7 |
2 |
181715548 |
- |
Oprl1 |
NNNAGACAAAGGCATACACA |
NGG |
2 |
0.4688 |
Tier II |
| 5 |
NC_000076.6 |
10 |
83587897 |
+ |
Washc4 |
NNNACACACAGTCATACACG |
NGG |
2 |
0.449 |
Tier II |
| 6 |
NC_000068.7 |
2 |
162607957 |
+ |
Ptprt |
NNNAGAGAAAGACATACACG |
NGG |
2 |
0.3765 |
Tier II |
| 7 |
NC_000072.6 |
6 |
105853891 |
- |
Cntn4 |
NNNAGAAAAAGTCATACATG |
NGG |
2 |
0.3462 |
Tier II |
| 8 |
NC_000069.6 |
3 |
129611085 |
- |
Elovl6 |
NNNAGACAAAGGCATAAACG |
NGG |
2 |
0.2333 |
Tier II |
| 9 |
NC_000085.6 |
19 |
18967074 |
- |
Rorb |
NNNAGACAAAGACATACACG |
NAG |
2 |
0.2074 |
Tier II |
| 10 |
NC_000080.6 |
14 |
31411173 |
+ |
Sh3bp5 |
NNNAGACAAAGCCATGCACG |
NGG |
2 |
0.1822 |
Tier II |
| 11 |
NC_000079.6 |
13 |
36400963 |
+ |
Fars2 |
NNNAGACAAACTCACACACG |
NGG |
2 |
0.1169 |
Tier II |
| 12 |
NC_000071.6 |
5 |
131784752 |
+ |
Auts2 |
NNNAGACAAAGTCATACACA |
NGA |
2 |
0.0651 |
Tier II |
| 13 |
NC_000078.6 |
12 |
84381924 |
+ |
Entpd5 |
NNNATACAAAGTCAGACACG |
NGG |
2 |
0.015 |
Tier II |
| 14 |
NC_000070.6 |
4 |
108265173 |
- |
Zyg11b |
NNNAGACAAAGTCATACACG |
NAA |
2 |
0.0 |
Tier II |
| 15 |
NC_000074.6 |
8 |
11331602 |
- |
Gm46030 |
NNNAAACAAAGTCATACACT |
NGG |
2 |
0.6067 |
Tier III |
| 16 |
NC_000078.6 |
12 |
29151186 |
+ |
Gm31333 |
NNNAGACAAAGTCTTACACT |
NGG |
2 |
0.3733 |
Tier III |
| 17 |
NC_000072.6 |
6 |
31189572 |
- |
Lncpint |
NNNAGACAAAGTCATACACA |
NGA |
2 |
0.0651 |
Tier III |
Other clones with same target sequence:
(none)
