Construct: sgRNA BRDN0001145134

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAAGCTGTTCATGTGAACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLT3 (2322)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77350

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 28049684 - FLT3 NNNGCTGTTCATGTGAACCA NGG 0 1.0 Tier I
2 NC_000013.11 13 23332702 + SACS NNNGCTGTTCTTGTGGACCA NGG 2 0.0592 Tier I
3 NC_000004.12 4 25007784 - LGI2 NNNGCTGTCCATGTGCACCA NGG 2 0.0 Tier I
4 NC_000003.12 3 17245893 - TBC1D5 NNNGCTGCTCGTGTGAACCA NGG 2 0.65 Tier II
5 NC_000008.11 8 134611260 - ZFAT NNNGCTTTTTATGTGAACCA NGG 2 0.5378 Tier II
6 NC_000001.11 1 15039499 + KAZN NNNGCTGTCCCTGTGAACCA NGG 2 0.3692 Tier II
7 NC_000021.9 21 25642083 + JAM2 NNNGCTGTTGATGTGAACCT NGG 2 0.2333 Tier II
8 NC_000007.14 7 96484429 + SEM1 NNNGCTCTTCATGTGAACCA NAG 2 0.1782 Tier II
9 NC_000009.12 9 92966682 + FGD3 NNNGCTGTGCATGTGGACCA NGG 2 0.119 Tier II
10 NC_000008.11 8 25822353 - LOC107986933 NNNGCTGTTCATGAAAACCA NGG 2 0.5826 Tier III
11 NC_000017.11 17 3977562 - LINC01975 NNNGCAGTTCGTGTGAACCA NGG 2 0.5633 Tier III
12 NC_000012.12 12 55580716 - LOC100418940 NNNGCTGTTCAAGTGAACCT NGG 2 0.48 Tier III
13 NC_000012.12 12 22926085 - LINC02955 NNNGCTCTGCATGTGAACCA NGG 2 0.4256 Tier III
14 NC_000007.14 7 96484429 + LOC105375411 NNNGCTCTTCATGTGAACCA NAG 2 0.1782 Tier III
15 NC_000021.9 21 22294750 + LOC107985508 NNNGGTGTTCATGTGACCCA NGG 2 0.1059 Tier III
16 NC_000021.9 21 42050295 + LOC107985502 NNNGCTGTTCATGTGGTCCA NGG 2 0.0256 Tier III
17 NC_000004.12 4 25007784 - LOC102723675 NNNGCTGTCCATGTGCACCA NGG 2 0.0 Tier III
18 NC_000018.10 18 52208359 - LOC105372121 NNNGCTGTTCATATGCACCA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 181653241 - Prpf6 NNNGCAGTTCATATGAACCA NGG 2 0.8 Tier II
2 NC_000072.6 6 127929890 + Tspan11 NNNGATGGTCATGTGAACCA NGG 2 0.419 Tier II
3 NC_000071.6 5 96999183 - Bmp2k NNNGCTGTTAATGTGAACAA NGG 2 0.3714 Tier II
4 NC_000071.6 5 128254352 + Tmem132d NNNACTGTTCATTTGAACCA NGG 2 0.27 Tier II
5 NC_000072.6 6 24858028 - Hyal5 NNNTGTGTTCATGTGAACCA NGG 2 0.2182 Tier II
6 NC_000077.6 11 22091776 + Ehbp1 NNNGCTGTACATGTGAGCCA NGG 2 0.1639 Tier II
7 NC_000068.7 2 14608308 + Cacnb2 NNNGCTGTTCATGTGAAACA NAG 2 0.1396 Tier II
8 NC_000081.6 15 100707048 - Galnt6 NNNGCTGTGCATGTGAGCCA NGG 2 0.1092 Tier II
9 NC_000081.6 15 100707130 - Galnt6 NNNGCTGTGCATGTGAGCCA NGG 2 0.1092 Tier II
10 NC_000081.6 15 100707422 - Galnt6 NNNGCTGTGCATGTGAGCCA NGG 2 0.1092 Tier II
11 NC_000071.6 5 86713515 - Tmprss11e NNNGCTGTTCATGTGAACTC NGG 2 0.1049 Tier II
12 NC_000071.6 5 53124270 + Sel1l3 NNNGCTGCTCATGTGAACCA NGA 2 0.0694 Tier II
13 NC_000074.6 8 28770643 - Unc5d NNNGCTGTTCATCTGAAGCA NGG 2 0.0561 Tier II
14 NC_000079.6 13 30834477 - Exoc2 NNNGCTGTTGATGTGATCCA NGG 2 0.0519 Tier II
15 NC_000069.6 3 37364883 - Fgf2 NNNGCTGTTCATGTGAAGCC NGG 2 0.0303 Tier II
16 NC_000071.6 5 144203208 - Tecpr1 NNNGGTGTTCATGTGAACCA NGC 2 0.0133 Tier II
17 NC_000081.6 15 96218063 + Gm38469 NNNGCTGTTCTTGTGAACCA NTG 2 0.012 Tier II
18 NC_000067.6 1 165436326 + Dcaf6 NNNGCTGTTCATGTGAACCA NTC 2 0.0 Tier II
19 NC_000075.6 9 119576159 - Scn5a NNNGCTGTTCATTGGAACCA NGG 2 0.0 Tier II
20 NC_000072.6 6 127929890 + Gm38903 NNNGATGGTCATGTGAACCA NGG 2 0.419 Tier III
21 NC_000068.7 2 21914770 - Gm39767 NNNGCTTGTCATGTGAACCA NGG 2 0.419 Tier III
22 NC_000081.6 15 10788449 + 4930556M19Rik NNNACTGTTGATGTGAACCA NGG 2 0.35 Tier III
23 NC_000083.6 17 94831572 - Gm1976 NNNGATGTTCATGTGAAGCA NGG 2 0.0762 Tier III
Download CSV

Other clones with same target sequence:

(none)