Construct: sgRNA BRDN0001145163
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACCAGGATTCTATTACTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAF1 (6872)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77871
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
71375282 |
+ |
TAF1 |
NNNCAGGATTCTATTACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
39372822 |
+ |
SYNGR1 |
NNNCAGGATTCAATTACTTG |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000002.12 |
2 |
57946589 |
- |
VRK2 |
NNNCAGGATTCTTTTAATGG |
NGG |
2 |
0.14 |
Tier II |
| 4 |
NC_000002.12 |
2 |
133389875 |
- |
NCKAP5 |
NNNCAGGATTCTATGACTGA |
NGG |
2 |
0.0469 |
Tier II |
| 5 |
NC_000008.11 |
8 |
9762059 |
- |
TNKS |
NNNCAGGATTCTATTACAGG |
NGT |
2 |
0.0108 |
Tier II |
| 6 |
NC_000001.11 |
1 |
168715016 |
+ |
DPT |
NNNCAGAATTCTATTCCTGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000013.11 |
13 |
99286088 |
+ |
UBAC2 |
NNNCAGGAGTCTATTCCTGG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000007.14 |
7 |
74993349 |
+ |
CASTOR2 |
NNNCAGGATTCCAGTACTGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000010.11 |
10 |
65991775 |
- |
CTNNA3 |
NNNCAGGATTCTAGTACTGA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000007.14 |
7 |
99286036 |
- |
MYH16 |
NNNCAGGATTCTATGCCTGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000016.10 |
16 |
73016607 |
- |
ZFHX3 |
NNNCAGGATTCTATTTCTGG |
NGT |
2 |
0.0 |
Tier II |
| 12 |
NC_000006.12 |
6 |
78248365 |
- |
LOC105377865 |
NNNCAGCATTCTATTTCTGG |
NGG |
2 |
0.0 |
Tier III |
| 13 |
NC_000007.14 |
7 |
141542251 |
- |
AGK-DT |
NNNCAGGATTCCATTCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 14 |
NC_000007.14 |
7 |
99286036 |
- |
LOC105375421 |
NNNCAGGATTCTATGCCTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
119067289 |
+ |
Cacna1c |
NNNCAGGATTTCATTACTGG |
NGG |
2 |
0.2915 |
Tier II |
| 2 |
NC_000072.6 |
6 |
96634538 |
+ |
Tafa1 |
NNNCAGGCTTCTGTTACTGG |
NGG |
2 |
0.2795 |
Tier II |
| 3 |
NC_000084.6 |
18 |
74654689 |
- |
Myo5b |
NNNCAGGCTTCTATCACTGG |
NGG |
2 |
0.1169 |
Tier II |
| 4 |
NC_000082.6 |
16 |
97420020 |
- |
Bace2 |
NNNCAGGTTTCTATTGCTGG |
NGG |
2 |
0.0824 |
Tier II |
| 5 |
NC_000067.6 |
1 |
19226749 |
+ |
Tfap2b |
NNNCAGGATTCTATTAGTGG |
NGG |
1 |
0.0588 |
Tier II |
| 6 |
NC_000067.6 |
1 |
172959997 |
- |
Gm35686 |
NNNCAGAATTCTATTAGTGG |
NGG |
2 |
0.0588 |
Tier III |
| 7 |
NC_000080.6 |
14 |
52779776 |
+ |
Tcra |
NNNCAGGATTCTAGTGCTGG |
NGG |
2 |
0.0 |
Tier III |
| 8 |
NC_000080.6 |
14 |
53471065 |
+ |
Tcra |
NNNCAGGATTCTAGTGCTGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
