Construct: sgRNA BRDN0001145167

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATAACTGAGACACCAAACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK1 (4750)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76128

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 169585458 + NEK1 NNNACTGAGACACCAAACTG NGG 0 1.0 Tier I
2 NC_000004.12 4 73449369 - AFP NNNACTGAGACAGCAAGCTG NGG 2 0.0241 Tier I
3 NC_000005.10 5 138171361 - BRD8 NNNACTGAGACACCAAAGTG NGT 2 0.0022 Tier I
4 NC_000007.14 7 139302993 - UBN2 NNNACTTAGACACCAAACTG NGG 1 0.5714 Tier II
5 NC_000016.10 16 77851095 + VAT1L NNNACAGAAACACCAAACTG NGG 2 0.5571 Tier II
6 NC_000016.10 16 53966516 - FTO NNNACTGAGAAATCAAACTG NGG 2 0.525 Tier II
7 NC_000021.9 21 31195505 - TIAM1 NNNACTTAGAAACCAAACTG NGG 2 0.4286 Tier II
8 NC_000004.12 4 26937874 - STIM2 NNNACTGATAAACCAAACTG NGG 2 0.4 Tier II
9 NC_000001.11 1 3466281 - ARHGEF16 NNNCCCGAGACACCAAACTG NGG 2 0.3209 Tier II
10 NC_000011.10 11 22741996 - GAS2 NNNACTAAGACCCCAAACTG NGG 2 0.2632 Tier II
11 NC_000001.11 1 156948153 + ARHGEF11 NNNACAGAGACACCAAACAG NGG 2 0.2476 Tier II
12 NC_000016.10 16 73320146 - ZFHX3 NNNACTGAGACACAGAACTG NGG 2 0.2275 Tier II
13 NC_000014.9 14 69134133 + DCAF5 NNNACTGAGGCACAAAACTG NGG 2 0.1944 Tier II
14 NC_000017.11 17 51097680 + SPAG9 NNNACTGAGACCTCAAACTG NGG 2 0.1842 Tier II
15 NC_000011.10 11 44269621 - ALX4 NNNGCTGAGACCCCAAACTG NGG 2 0.1645 Tier II
16 NC_000012.12 12 104556031 - CHST11 NNNACTGAGACCCCAAAATG NGG 2 0.1417 Tier II
17 NC_000006.12 6 87214032 - ZNF292 NNNACTGATACCCCAAACTG NGG 2 0.1404 Tier II
18 NC_000001.11 1 171965044 - DNM3 NNNACTGAGACATCAACCTG NGG 2 0.1235 Tier II
19 NC_000010.11 10 112714140 + VTI1A NNNACTGAGAAACCAATCTG NGG 2 0.1 Tier II
20 NC_000008.11 8 140242205 + TRAPPC9 NNNACTTAGACACCAAAGTG NGG 2 0.0762 Tier II
21 NC_000017.11 17 7680122 + TP53 NNNACTGAGACCCCAAACAG NGG 2 0.0752 Tier II
22 NC_000003.12 3 25586354 - RARB NNNACTGAGACAACAGACTG NGG 2 0.074 Tier II
23 NC_000008.11 8 88248098 + MMP16 NNNACTGAGACACAAAGCTG NGG 2 0.0618 Tier II
24 NC_000011.10 11 107383287 + CWF19L2 NNNACTGAGACCCCAACCTG NGG 2 0.0464 Tier II
25 NC_000016.10 16 84719892 + USP10 NNNACTGAGACAGCAAACAG NGG 2 0.039 Tier II
26 NC_000016.10 16 61815721 - CDH8 NNNACTGAGACACCAATCAG NGG 2 0.0381 Tier II
27 NC_000008.11 8 134554129 - ZFAT NNNACTGAGACAGCAGACTG NGG 2 0.0262 Tier II
28 NC_000004.12 4 865275 - GAK NNNGCTGAGACACCAAACTG NGC 2 0.0139 Tier II
29 NC_000001.11 1 54371528 - SSBP3 NNNACTGAAACACCAAACTG NGT 2 0.0104 Tier II
30 NC_000001.11 1 180688794 - XPR1 NNNACTGAGACAGCAAACTG NGA 2 0.0095 Tier II
31 NC_000016.10 16 89743341 - FANCA NNNACTCAGACACCACACTG NGG 2 0.0 Tier II
32 NC_000004.12 4 24881709 + CCDC149 NNNACTGAGTCACCACACTG NGG 2 0.0 Tier II
33 NC_000005.10 5 138585010 + LOC105379193 NNNTCTCAGACACCAAACTG NGG 2 0.4375 Tier III
34 NC_000008.11 8 97345100 - LOC101927066 NNNACTTTGACACCAAACTG NGG 2 0.2449 Tier III
35 NC_000005.10 5 169573471 + LOC105377714 NNNACTGAAACACCAAACAG NGG 2 0.1837 Tier III
36 NC_000022.11 22 23928218 - KLHL5P1 NNNACTGCGACACCAAACTG NAG 2 0.1111 Tier III
37 NC_000023.11 X 139362303 + SNURFL NNNACTGAGACACCAAGATG NGG 2 0.095 Tier III
38 NC_000011.10 11 131076196 + LOC105369579 NNNCCTGAGACCCCAAACTG NGG 2 0.0929 Tier III
39 NC_000011.10 11 131076196 + LOC107984411 NNNCCTGAGACCCCAAACTG NGG 2 0.0929 Tier III
40 NC_000001.11 1 1906302 - LOC124903822 NNNACTAAGACACCACACTG NGG 2 0.0 Tier III
41 NC_000006.12 6 29298731 + LOC105375005 NNNACTGAGACACCATATTG NGG 2 0.0 Tier III
42 NC_000003.12 3 168555424 - EGFEM1P NNNACTGAGTCACCATACTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 61028751 - Nek1 NNNACTGAGACAGCAAGCTG NGG 2 0.0241 Tier I
2 NC_000068.7 2 119134387 - Rad51 NNNACTGCGACACCAAACTG NTG 2 0.0167 Tier I
3 NC_000067.6 1 132511279 + Cntn2 NNNACTGAAAAACCAAACTG NGG 2 0.4821 Tier II
4 NC_000069.6 3 10426952 + Snx16 NNNACTGACACACTAAACTG NGG 2 0.3949 Tier II
5 NC_000072.6 6 32933517 + Chchd3 NNNAATGAGGCACCAAACTG NGG 2 0.3175 Tier II
6 NC_000073.6 7 126962843 - Sez6l2 NNNAATGAGGCACCAAACTG NGG 2 0.3175 Tier II
7 NC_000068.7 2 6352537 - Usp6nl NNNACTTACACACCAAACTG NGG 2 0.3077 Tier II
8 NC_000077.6 11 103824224 + Nsf NNNCCTGAGAAACCAAACTG NGG 2 0.2647 Tier II
9 NC_000071.6 5 23665822 - Srpk2 NNNACTGAGAAACCAAACAG NGG 2 0.2143 Tier II
10 NC_000084.6 18 67490418 + Spire1 NNNACTCAGATACCAAACTG NGG 2 0.2115 Tier II
11 NC_000079.6 13 20946190 - Aoah NNNACTGAGACGCCAAACAG NGG 2 0.2063 Tier II
12 NC_000067.6 1 170560998 + Nos1ap NNNAGTGAGCCACCAAACTG NGG 2 0.2 Tier II
13 NC_000082.6 16 4852924 + 4930562C15Rik NNNACTGAGAGGCCAAACTG NGG 2 0.1806 Tier II
14 NC_000074.6 8 61374528 - Sh3rf1 NNNACTGAGACATCAGACTG NGG 2 0.1346 Tier II
15 NC_000069.6 3 88319851 - Cct3 NNNACTGAGACACCAAAGTG NGG 1 0.1333 Tier II
16 NC_000082.6 16 75168255 + Robo2 NNNACTGAGAAACCAAGCTG NGG 2 0.1324 Tier II
17 NC_000068.7 2 140743963 + Macrod2 NNNACTGAGCCACAAAACTG NGG 2 0.1167 Tier II
18 NC_000082.6 16 95445280 + Erg NNNAATGAGACACCAGACTG NGG 2 0.1099 Tier II
19 NC_000071.6 5 31283587 + Ift172 NNNACTGAGACACAAAACAG NGG 2 0.1 Tier II
20 NC_000068.7 2 93731176 + Ext2 NNNCCTGAGACACCAAACTG NAG 2 0.0915 Tier II
21 NC_000073.6 7 143149320 + Kcnq1 NNNATTGAGACACCAATCTG NGG 2 0.0848 Tier II
22 NC_000075.6 9 31308145 - Prdm10 NNNACTGAGCCACCAACCTG NGG 2 0.0588 Tier II
23 NC_000084.6 18 73980605 + Mapk4 NNNACTGAGACACCCAGCTG NGG 2 0.0481 Tier II
24 NC_000071.6 5 92842747 - Shroom3 NNNACTGAGACAGCAAACAG NGG 2 0.039 Tier II
25 NC_000081.6 15 55450428 + Col14a1 NNNACTGAGACACCCATCTG NGG 2 0.0364 Tier II
26 NC_000067.6 1 131702942 + Rab7b NNNACTGAGACACCAATCTG NAG 2 0.0346 Tier II
27 NC_000076.6 10 80667436 + Mknk2 NNNGCTGAGACACCAAACTG NTG 2 0.0244 Tier II
28 NC_000076.6 10 70995893 - Bicc1 NNNACTGAGACACAAAACTG NTG 2 0.0136 Tier II
29 NC_000072.6 6 141025548 + Gm30524 NNNACTGAGTCACCAAATTG NGG 2 0.5672 Tier III
30 NC_000070.6 4 12184211 - Gm46846 NNNGCTGGGACACCAAACTG NGG 2 0.4583 Tier III
31 NC_000079.6 13 20946190 - Gm32242 NNNACTGAGACGCCAAACAG NGG 2 0.2063 Tier III
32 NC_000084.6 18 83086745 - Gm46636 NNNACTGAGGCACCAGACTG NGG 2 0.1068 Tier III
33 NC_000078.6 12 114278366 + Igh NNNACTGAGACACCAACCTG NAG 2 0.0458 Tier III
34 NC_000081.6 15 99255966 - 1700120C14Rik NNNACTGAGACACCAATCTG NGA 2 0.0093 Tier III
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Other clones with same target sequence:

(none)