Construct: sgRNA BRDN0001145227
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGCCAGAAGGATACCAGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAK (4117)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76711
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
10802038 |
- |
MAK |
NNNGCCAGAAGGATACCAGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
127582037 |
+ |
GCC1 |
NNNGCCAGAAGGCCACCAGC |
NGG |
2 |
0.0602 |
Tier I |
| 3 |
NC_000008.11 |
8 |
142680631 |
+ |
PSCA |
NNNGCCAGAGGGATACCTGC |
NGG |
2 |
0.2778 |
Tier II |
| 4 |
NC_000014.9 |
14 |
64072801 |
- |
SYNE2 |
NNNGCCAGAAGTGTACCAGC |
NGG |
2 |
0.2508 |
Tier II |
| 5 |
NC_000002.12 |
2 |
162481009 |
- |
KCNH7 |
NNNGCCAGAAGGATGTCAGC |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000019.10 |
19 |
16944817 |
+ |
CPAMD8 |
NNNGCCAGCAGGATCCCAGC |
NGG |
2 |
0.1558 |
Tier II |
| 7 |
NC_000005.10 |
5 |
35134604 |
- |
PRLR |
NNNGCCAGAAGGCAACCAGC |
NGG |
2 |
0.1303 |
Tier II |
| 8 |
NC_000001.11 |
1 |
6300053 |
+ |
ACOT7 |
NNNGCCAGCAGGATTCCAGC |
NGG |
2 |
0.1143 |
Tier II |
| 9 |
NC_000016.10 |
16 |
79006834 |
- |
WWOX |
NNNGCCAGAAGGATGCCAGC |
NTG |
2 |
0.0253 |
Tier II |
| 10 |
NC_000007.14 |
7 |
127582037 |
+ |
LOC105375490 |
NNNGCCAGAAGGCCACCAGC |
NGG |
2 |
0.0602 |
Tier III |
| 11 |
NC_000006.12 |
6 |
14452514 |
- |
LOC101928331 |
NNNGCCAGAAGGATAACAGC |
NTG |
2 |
0.039 |
Tier III |
| 12 |
NC_000006.12 |
6 |
14452514 |
- |
LOC105374942 |
NNNGCCAGAAGGATAACAGC |
NTG |
2 |
0.039 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
41048199 |
- |
Mak |
NNNGCCGGAAGGATACCAGC |
NTG |
2 |
0.0275 |
Tier I |
| 2 |
NC_000077.6 |
11 |
119547356 |
- |
Nptx1 |
NNNGCCAGAAGGAGACCATC |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000070.6 |
4 |
106991456 |
+ |
Ssbp3 |
NNNGCTAGAAGGATGCCAGC |
NGG |
2 |
0.6036 |
Tier II |
| 4 |
NC_000067.6 |
1 |
126692531 |
- |
Nckap5 |
NNNGCCAGAAGGATAACACC |
NGG |
2 |
0.4483 |
Tier II |
| 5 |
NC_000071.6 |
5 |
36383842 |
- |
Sorcs2 |
NNNGTCAGAAGGATGCCAGC |
NGG |
2 |
0.4136 |
Tier II |
| 6 |
NC_000068.7 |
2 |
168753716 |
- |
Sall4 |
NNNGGCAGGAGGATACCAGC |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000078.6 |
12 |
103115775 |
- |
Unc79 |
NNNGCCAGCAGGAAACCAGC |
NGG |
2 |
0.3537 |
Tier II |
| 8 |
NC_000075.6 |
9 |
118989718 |
- |
Ctdspl |
NNNGCTAGAAGGATCCCAGC |
NGG |
2 |
0.2532 |
Tier II |
| 9 |
NC_000067.6 |
1 |
168417901 |
+ |
Pbx1 |
NNNTCCAGAAGGATGCCAGC |
NGG |
2 |
0.2364 |
Tier II |
| 10 |
NC_000072.6 |
6 |
72324917 |
- |
Usp39 |
NNNGCCAGAAGTATACCAGA |
NGG |
2 |
0.1923 |
Tier II |
| 11 |
NC_000075.6 |
9 |
63231849 |
+ |
Map2k5 |
NNNGCCAGAAGTATACTAGC |
NGG |
2 |
0.1795 |
Tier II |
| 12 |
NC_000068.7 |
2 |
26931313 |
+ |
Surf4 |
NNNGCCAGCAGGATCCCAGC |
NGG |
2 |
0.1558 |
Tier II |
| 13 |
NC_000081.6 |
15 |
48195284 |
- |
Csmd3 |
NNNGCCAGAAGGATTCTAGC |
NGG |
2 |
0.0933 |
Tier II |
| 14 |
NC_000080.6 |
14 |
33260271 |
+ |
Arhgap22 |
NNNGCCAGAGGGATACCAGC |
NTG |
2 |
0.0216 |
Tier II |
| 15 |
NC_000073.6 |
7 |
40898965 |
+ |
Vstm2b |
NNNGCCAGAAGGAGACCAGG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000070.6 |
4 |
155852567 |
- |
Dvl1 |
NNNGCCAGAAGGATACCAGC |
NCT |
2 |
0.0 |
Tier II |
| 17 |
NC_000071.6 |
5 |
36368661 |
+ |
Sorcs2 |
NNNGCCAGGAGGAGACCAGC |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000069.6 |
3 |
58025745 |
+ |
Gm40055 |
NNNGCCAGAAGAATTCCAGC |
NGG |
2 |
0.1867 |
Tier III |
| 19 |
NC_000068.7 |
2 |
26931313 |
+ |
Gm36591 |
NNNGCCAGCAGGATCCCAGC |
NGG |
2 |
0.1558 |
Tier III |
| 20 |
NC_000068.7 |
2 |
115030286 |
+ |
Gm13974 |
NNNGCCAGCAGGATACCAGC |
NGC |
2 |
0.0127 |
Tier III |
Other clones with same target sequence:
(none)
