Construct: sgRNA BRDN0001145233
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCTTTGGAAGCCCTAACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RBKS (64080)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77041
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
27843088 |
- |
RBKS |
NNNTTTGGAAGCCCTAACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
99342974 |
- |
FAXC |
NNNTTTGCAAGACCTAACAA |
NGG |
2 |
0.4396 |
Tier I |
| 3 |
NC_000020.11 |
20 |
31975174 |
- |
XKR7 |
NNNTTTGGATGCCCTAACAA |
NGG |
1 |
0.8824 |
Tier II |
| 4 |
NC_000002.12 |
2 |
159153963 |
+ |
TANC1 |
NNNTTTGGAAGACCTAACAG |
NGG |
2 |
0.5462 |
Tier II |
| 5 |
NC_000010.11 |
10 |
7332808 |
+ |
SFMBT2 |
NNNCTTGGAAGCCTTAACAA |
NGG |
2 |
0.4745 |
Tier II |
| 6 |
NC_000016.10 |
16 |
4520455 |
+ |
CDIP1 |
NNNATTGGAAGCCTTAACAA |
NGG |
2 |
0.3492 |
Tier II |
| 7 |
NC_000002.12 |
2 |
214004617 |
+ |
SPAG16 |
NNNTTTGGCAGCCCTAAAAA |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000004.12 |
4 |
113298189 |
- |
ANK2 |
NNNTTTTGAAGTCCTAACAA |
NGG |
2 |
0.3077 |
Tier II |
| 9 |
NC_000008.11 |
8 |
120585106 |
+ |
SNTB1 |
NNNTATGGCAGCCCTAACAA |
NGG |
2 |
0.2857 |
Tier II |
| 10 |
NC_000011.10 |
11 |
40722972 |
+ |
LRRC4C |
NNNTTTGGAAGCCTTAACAA |
NAG |
2 |
0.1901 |
Tier II |
| 11 |
NC_000003.12 |
3 |
40169483 |
- |
MYRIP |
NNNTCTGGAAGCCCTATCAA |
NGG |
2 |
0.1333 |
Tier II |
| 12 |
NC_000012.12 |
12 |
53338993 |
+ |
SP7 |
NNNATTGGAAGCCCTAACCA |
NGG |
2 |
0.0985 |
Tier II |
| 13 |
NC_000020.11 |
20 |
1657269 |
+ |
SIRPG |
NNNTGTGGAAGCCCTAAGAA |
NGG |
2 |
0.0853 |
Tier II |
| 14 |
NC_000011.10 |
11 |
88058870 |
- |
RAB38 |
NNNTTTGGAAGCCCAAAGAA |
NGG |
2 |
0.0772 |
Tier II |
| 15 |
NC_000014.9 |
14 |
74899678 |
- |
DLST |
NNNTTTGGCAGCCCTAAGAA |
NGG |
2 |
0.0762 |
Tier II |
| 16 |
NC_000010.11 |
10 |
51538665 |
+ |
PRKG1 |
NNNTTTGGAAACCCTAACAA |
NGA |
2 |
0.0694 |
Tier II |
| 17 |
NC_000017.11 |
17 |
9784854 |
+ |
DHRS7C |
NNNTTTTGAAGCCCTAACAA |
NTG |
2 |
0.0223 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
21936693 |
+ |
Faxc |
NNNTTTGCAAGACCTAACAA |
NGG |
2 |
0.4396 |
Tier I |
| 2 |
NC_000076.6 |
10 |
92815792 |
+ |
Cfap54 |
NNNTCTGGAAGCCCTAACAA |
NGG |
1 |
1.0 |
Tier II |
| 3 |
NC_000076.6 |
10 |
91048417 |
- |
Apaf1 |
NNNTTTGCAAGCCTTAACAA |
NGG |
2 |
0.4513 |
Tier II |
| 4 |
NC_000077.6 |
11 |
104322136 |
+ |
Mapt |
NNNTTTGCAAGCCCTAAAAA |
NGG |
2 |
0.3314 |
Tier II |
| 5 |
NC_000068.7 |
2 |
146194355 |
- |
Cfap61 |
NNNTTTGTAAGGCCTAACAA |
NGG |
2 |
0.2778 |
Tier II |
| 6 |
NC_000082.6 |
16 |
72753598 |
- |
Robo1 |
NNNTTTGGAAACCCTAACAC |
NGG |
2 |
0.2273 |
Tier II |
| 7 |
NC_000072.6 |
6 |
139833649 |
- |
Pik3c2g |
NNNGTTGGAAGCCCTAACAC |
NGG |
2 |
0.142 |
Tier II |
| 8 |
NC_000067.6 |
1 |
24522454 |
+ |
Col19a1 |
NNNTTTGGCAGCCCTAGCAA |
NGG |
2 |
0.1008 |
Tier II |
| 9 |
NC_000071.6 |
5 |
22249027 |
+ |
Reln |
NNNTTTGGAAGCACTAACAA |
NAG |
2 |
0.0997 |
Tier II |
| 10 |
NC_000081.6 |
15 |
79767407 |
+ |
Dnal4 |
NNNTTTGGAGGCCCGAACAA |
NGG |
2 |
0.0278 |
Tier II |
| 11 |
NC_000073.6 |
7 |
50639921 |
- |
Nell1 |
NNNTGTGGAAGCCCTCACAA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000073.6 |
7 |
131471716 |
+ |
Gm39099 |
NNNTTTGGAAGCCCTAAATA |
NGG |
2 |
0.2899 |
Tier III |
| 13 |
NC_000073.6 |
7 |
71956692 |
- |
Gm36633 |
NNNTTTGGGAGCCCTATCAA |
NGG |
2 |
0.0889 |
Tier III |
Other clones with same target sequence:
(none)
