Construct: sgRNA BRDN0001145260
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTATCGTGTTCAACCAGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPK1 (3705)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77150
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
92958347 |
- |
ITPK1 |
NNNATCGTGTTCAACCAGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
135867833 |
- |
MCM6 |
NNNATCGTGTTAAACCAGGA |
NGG |
1 |
0.7143 |
Tier II |
| 3 |
NC_000002.12 |
2 |
222925054 |
- |
ACSL3 |
NNNACCGTGTTCAGCCAGGA |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000012.12 |
12 |
122510337 |
- |
RSRC2 |
NNNACCGTGTTGAACCAGGA |
NGG |
2 |
0.4444 |
Tier II |
| 5 |
NC_000006.12 |
6 |
98179292 |
+ |
EIF4EBP2P3 |
NNNATCCTGTTTAACCAGGA |
NGG |
2 |
0.3702 |
Tier III |
| 6 |
NC_000010.11 |
10 |
120624685 |
- |
LINC02930 |
NNNATCTTGTTCAAGCAGGA |
NGG |
2 |
0.0286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
102584433 |
- |
Itpk1 |
NNNATTGTGTTCAACCAAGA |
NGG |
2 |
0.6429 |
Tier I |
| 2 |
NC_000077.6 |
11 |
107638839 |
+ |
Helz |
NNNATCGTATTCAACCAGCA |
NGG |
2 |
0.2882 |
Tier II |
| 3 |
NC_000082.6 |
16 |
59497027 |
- |
Crybg3 |
NNNATCTTGTTCTACCAGGA |
NGG |
2 |
0.1714 |
Tier II |
| 4 |
NC_000069.6 |
3 |
131968566 |
+ |
Gm29865 |
NNNATCGTGTTCATCCAGGA |
NAG |
2 |
0.1383 |
Tier III |
Other clones with same target sequence:
(none)
