Construct: sgRNA BRDN0001145261
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCATCCTACGAAGACTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKB (3707)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76874
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
226735616 |
- |
ITPKB |
NNNATCCTACGAAGACTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
81300421 |
+ |
IL16 |
NNNATCCAACGAAGACTCAG |
NTG |
2 |
0.0312 |
Tier I |
| 3 |
NC_000011.10 |
11 |
130873483 |
+ |
SNX19 |
NNNATACTACGTAGACTCAG |
NGG |
2 |
0.3095 |
Tier II |
| 4 |
NC_000008.11 |
8 |
103898143 |
- |
RIMS2 |
NNNATCCTACAAAGATTCAG |
NGG |
2 |
0.3077 |
Tier II |
| 5 |
NC_000007.14 |
7 |
102476282 |
+ |
POLR2J |
NNNATCCTAGGAAGACACAG |
NGG |
2 |
0.2074 |
Tier II |
| 6 |
NC_000007.14 |
7 |
102569936 |
+ |
POLR2J3 |
NNNATCCTAGGAAGACACAG |
NGG |
2 |
0.2074 |
Tier II |
| 7 |
NC_000007.14 |
7 |
102669016 |
+ |
POLR2J2 |
NNNATCCTAGGAAGACACAG |
NGG |
2 |
0.2074 |
Tier II |
| 8 |
NC_000012.12 |
12 |
44163679 |
- |
TMEM117 |
NNNATCCTAGGAAGACTCAG |
NGA |
2 |
0.027 |
Tier II |
| 9 |
NC_000014.9 |
14 |
98226825 |
+ |
LOC105370656 |
NNNATCTTACAAAGACTCAG |
NGG |
2 |
0.8125 |
Tier III |
| 10 |
NC_000007.14 |
7 |
44016535 |
+ |
POLR2J4 |
NNNATCCTAGGAAGACACAG |
NGG |
2 |
0.2074 |
Tier III |
| 11 |
NC_000007.14 |
7 |
102569936 |
+ |
POLR2J3-UPK3BL2 |
NNNATCCTAGGAAGACACAG |
NGG |
2 |
0.2074 |
Tier III |
| 12 |
NC_000007.14 |
7 |
102669016 |
+ |
POLR2J2-UPK3BL1 |
NNNATCCTAGGAAGACACAG |
NGG |
2 |
0.2074 |
Tier III |
| 13 |
NC_000006.12 |
6 |
113050721 |
+ |
LOC107986636 |
NNNATCCTACAAAGACTCAG |
NGC |
2 |
0.0222 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
103389138 |
+ |
Slc6a15 |
NNNGTCCAACGAAGACTCAG |
NGG |
2 |
0.5 |
Tier I |
| 2 |
NC_000070.6 |
4 |
66097548 |
+ |
Astn2 |
NNNATCCTATGAAGAATCAG |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000070.6 |
4 |
117410875 |
- |
Rnf220 |
NNNATCCTACAAGGACTCAG |
NGG |
2 |
0.6522 |
Tier II |
| 4 |
NC_000084.6 |
18 |
38309419 |
- |
Rnf14 |
NNNATCCTATGAAGACTCTG |
NGG |
2 |
0.5068 |
Tier II |
| 5 |
NC_000080.6 |
14 |
28106643 |
- |
Erc2 |
NNNATCCCAGGAAGACTCAG |
NGG |
2 |
0.3889 |
Tier II |
| 6 |
NC_000078.6 |
12 |
99432050 |
+ |
Foxn3 |
NNNATCCTGCTAAGACTCAG |
NGG |
2 |
0.2564 |
Tier II |
| 7 |
NC_000080.6 |
14 |
119395586 |
+ |
Hs6st3 |
NNNATGCTACCAAGACTCAG |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000069.6 |
3 |
68602411 |
- |
Schip1 |
NNNATCCTGCGAATACTCAG |
NGG |
2 |
0.1778 |
Tier II |
| 9 |
NC_000069.6 |
3 |
68602411 |
- |
Iqschfp |
NNNATCCTGCGAATACTCAG |
NGG |
2 |
0.1778 |
Tier II |
| 10 |
NC_000077.6 |
11 |
120239806 |
- |
Bahcc1 |
NNNATCCTACGCAGACTCAG |
NGA |
2 |
0.0183 |
Tier II |
| 11 |
NC_000074.6 |
8 |
91813977 |
- |
Irx3os |
NNNAACCTACAAAGACTCAG |
NGG |
2 |
0.5 |
Tier III |
| 12 |
NC_000080.6 |
14 |
104856910 |
- |
D130009I18Rik |
NNNATCCTACGAAGAGTCAA |
NGG |
2 |
0.1442 |
Tier III |
Other clones with same target sequence:
(none)
