Construct: sgRNA BRDN0001145278

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTGCATCACATCCAACTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK7 (122481)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77913

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 96456441 + AK7 NNNGCATCACATCCAACTGA NGG 0 1.0 Tier I
2 NC_000011.10 11 108162140 + NPAT NNNGCATGACATCCAACTGA NTG 2 0.025 Tier I
3 NC_000015.10 15 48878230 - EID1 NNNGCATCACATCCATCTGC NGG 2 0.0 Tier I
4 NC_000010.11 10 21043228 + NEBL NNNGCTTCACATCCAACAGA NGG 2 0.4762 Tier II
5 NC_000008.11 8 99564427 + VPS13B NNNGCAGCACATCCAACTGG NGG 2 0.4498 Tier II
6 NC_000018.10 18 62332222 + TNFRSF11A NNNGCATCACATCCCACAGA NGG 2 0.1818 Tier II
7 NC_000005.10 5 37713803 + WDR70 NNNGTATCACATCCCACTGA NGG 2 0.1736 Tier II
8 NC_000008.11 8 714967 - ERICH1 NNNGCAGCACATCCAACTGA NAG 2 0.1525 Tier II
9 NC_000011.10 11 108693411 - DDX10 NNNTCATCACATCAAACTGA NGG 2 0.1273 Tier II
10 NC_000002.12 2 29426099 - ALK NNNGCATCACATCCAGCTGT NGG 2 0.1154 Tier II
11 NC_000023.11 X 29951347 + IL1RAPL1 NNNTCATCACTTCCAACTGA NGG 2 0.1119 Tier II
12 NC_000012.12 12 25973465 - RASSF8 NNNCCATCACATCCAGCTGA NGG 2 0.1018 Tier II
13 NC_000012.12 12 2210378 - CACNA1C NNNTCATCACATCCCACTGA NGG 2 0.0992 Tier II
14 NC_000016.10 16 1722241 - MAPK8IP3 NNNGCATCACATGCAACTTA NGG 2 0.0909 Tier II
15 NC_000003.12 3 174275585 - NLGN1 NNNGCATCACATACTACTGA NGG 2 0.0769 Tier II
16 NC_000001.11 1 229619760 - TAF5L NNNGCATCACATCCAGCTGA NAG 2 0.0499 Tier II
17 NC_000010.11 10 16922297 + CUBN NNNGCCTCACATCCAACTGA NCG 2 0.0487 Tier II
18 NC_000016.10 16 85800485 - COX4I1 NNNGCATCACATTCAACTGA NTG 2 0.0273 Tier II
19 NC_000011.10 11 6264263 - CCKBR NNNGGATCACATCCAACTGA NTG 2 0.0234 Tier II
20 NC_000003.12 3 50355961 + TMEM115 NNNGCATCACATCCAAGGGA NGG 2 0.0196 Tier II
21 NC_000012.12 12 72589529 - TRHDE NNNGCATCACACCCATCTGA NGG 2 0.0 Tier II
22 NC_000015.10 15 48878230 - SHC4 NNNGCATCACATCCATCTGC NGG 2 0.0 Tier II
23 NC_000003.12 3 172225532 + FNDC3B NNNGCATCACATTCATCTGA NGG 2 0.0 Tier II
24 NC_000001.11 1 99464442 + LOC124904580 NNNGCACTACATCCAACTGA NGG 2 0.6016 Tier III
25 NC_000010.11 10 2013439 - LINC00700 NNNGCAGCAGATCCAACTGA NGG 2 0.2288 Tier III
26 NC_000005.10 5 129170909 - LOC102723654 NNNGCATCACATCAAACTCA NGG 2 0.1569 Tier III
27 NC_000006.12 6 1537511 + LOC102723944 NNNGCATCACATAAAACTGA NGG 2 0.1346 Tier III
28 NC_000003.12 3 50355961 + LOC127898564 NNNGCATCACATCCAAGGGA NGG 2 0.0196 Tier III
29 NC_000003.12 3 132386887 - G2E3P1 NNNGCAACACATCCAACTGA NGC 2 0.0194 Tier III
30 NC_000003.12 3 132386887 - LOC124909435 NNNGCAACACATCCAACTGA NGC 2 0.0194 Tier III
31 NC_000003.12 3 172225532 + RPS27AP8 NNNGCATCACATTCATCTGA NGG 2 0.0 Tier III
32 NC_000001.11 1 208323835 - LOC105372889 NNNGCATCTCATCCACCTGA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 140329756 - Sel1l2 NNNGTATGACATCCAACTGA NGG 2 0.4091 Tier II
2 NC_000067.6 1 89477623 - Agap1 NNNCCAGCACATCCAACTGA NGG 2 0.3114 Tier II
3 NC_000075.6 9 94780661 + Slc9a9 NNNGCCTCTCATCCAACTGA NGG 2 0.2727 Tier II
4 NC_000068.7 2 71996377 - Rapgef4 NNNGCATCAGATTCAACTGA NGG 2 0.2722 Tier II
5 NC_000082.6 16 96307491 + B3galt5 NNNGCATCCCCTCCAACTGA NGG 2 0.2286 Tier II
6 NC_000079.6 13 8726000 - Adarb2 NNNGCATCACATCCAACTGG NTG 2 0.0298 Tier II
7 NC_000068.7 2 160900349 + Lpin3 NNNGCATCACATTCAACTGA NTG 2 0.0273 Tier II
8 NC_000076.6 10 56100584 - Msl3l2 NNNGCATCACATCCAACTCA NTG 2 0.0175 Tier II
9 NC_000076.6 10 56100584 - Tbc1d32 NNNGCATCACATCCAACTCA NTG 2 0.0175 Tier II
10 NC_000069.6 3 157622124 + Ptger3 NNNGCATCACATGCATCTGA NGG 2 0.0 Tier II
11 NC_000083.6 17 45258095 - Gm41582 NNNACATCACATCCAATTGA NGG 2 0.42 Tier III
12 NC_000076.6 10 117892704 + Gm32605 NNNGCACCACATCCAACTGT NGG 2 0.4125 Tier III
13 NC_000070.6 4 125236906 + Gm32611 NNNGCATCACCTCCAACTGA NAG 2 0.1037 Tier III
14 NC_000082.6 16 50527924 + G730013B05Rik NNNACATCACATCCAACTGA NGA 2 0.0625 Tier III
15 NC_000076.6 10 56100584 - Gm29794 NNNGCATCACATCCAACTCA NTG 2 0.0175 Tier III
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Other clones with same target sequence:

(none)