Construct: sgRNA BRDN0001145290
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAACTCACATGAGTCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ZAP70 (7535)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77957
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
97733336 |
- |
ZAP70 |
NNNACTCACATGAGTCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
8977805 |
- |
MBOAT2 |
NNNACTCACATGGGTCAACT |
NGG |
2 |
0.4565 |
Tier II |
| 3 |
NC_000008.11 |
8 |
19540872 |
- |
CSGALNACT1 |
NNNACTCACATGAGTCATCC |
NGG |
2 |
0.2143 |
Tier II |
| 4 |
NC_000013.11 |
13 |
97391990 |
- |
MBNL2 |
NNNAATCACATGATTCAACG |
NGG |
2 |
0.1524 |
Tier II |
| 5 |
NC_000003.12 |
3 |
61942425 |
- |
PTPRG |
NNNACTCACATGAGTGAATG |
NGG |
2 |
0.071 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
54617223 |
- |
Acsbg1 |
NNNACACACATGAGTCAACA |
NGG |
2 |
0.8125 |
Tier II |
| 2 |
NC_000070.6 |
4 |
148562690 |
- |
Exosc10 |
NNNACACACATGAATCAACG |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000080.6 |
14 |
40840487 |
- |
Sh2d4b |
NNNACTCACATGGGTCAACT |
NGG |
2 |
0.4565 |
Tier II |
| 4 |
NC_000067.6 |
1 |
136073186 |
- |
Cacna1s |
NNNCCTGACATGAGTCAACG |
NGG |
2 |
0.1661 |
Tier II |
| 5 |
NC_000075.6 |
9 |
122181479 |
+ |
Ano10 |
NNNACACACATGAGTCTACG |
NGG |
2 |
0.1156 |
Tier II |
| 6 |
NC_000082.6 |
16 |
38608245 |
- |
Arhgap31 |
NNNACACACATGAGTCTACG |
NGG |
2 |
0.1156 |
Tier II |
| 7 |
NC_000085.6 |
19 |
48592477 |
+ |
Sorcs3 |
NNNACTCACATGAGGAAACG |
NGG |
2 |
0.05 |
Tier II |
| 8 |
NC_000082.6 |
16 |
85364400 |
- |
Gm41483 |
NNNACACACATGAGTCAATG |
NGG |
2 |
0.4 |
Tier III |
Other clones with same target sequence:
(none)
