Construct: sgRNA BRDN0001145294
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATCCTCGCTCGGGACTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALPK1 (80216)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76083
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
112411861 |
+ |
ALPK1 |
NNNCCTCGCTCGGGACTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
8448651 |
- |
TRMT44 |
NNNCCCTGCTCGGGACTGTG |
NGG |
2 |
0.7386 |
Tier II |
| 3 |
NC_000017.11 |
17 |
8867582 |
- |
PIK3R6 |
NNNCCTCGCTCAGGACCGTG |
NGG |
2 |
0.6588 |
Tier II |
| 4 |
NC_000009.12 |
9 |
133840883 |
- |
VAV2 |
NNNCCTCCCTCTGGACTGTG |
NGG |
2 |
0.2367 |
Tier II |
| 5 |
NC_000016.10 |
16 |
13424097 |
- |
SHISA9 |
NNNCCTCTCTTGGGACTGTG |
NGG |
2 |
0.1923 |
Tier II |
| 6 |
NC_000002.12 |
2 |
30232737 |
- |
LBH |
NNNCCTCGCTCGGGGCTGTG |
NCG |
2 |
0.0696 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
46834302 |
+ |
Gabbr2 |
NNNCCTCGCTCGACACTGTG |
NGG |
2 |
0.3956 |
Tier II |
| 2 |
NC_000074.6 |
8 |
122163196 |
+ |
Zfp469 |
NNNCCTCCCTCTGGACTGTG |
NGG |
2 |
0.2367 |
Tier II |
| 3 |
NC_000080.6 |
14 |
117921342 |
+ |
Gpc6 |
NNNCCTGGCTCGGCACTGTG |
NGG |
2 |
0.2017 |
Tier II |
| 4 |
NC_000078.6 |
12 |
110565890 |
+ |
Ppp2r5c |
NNNCCTCGCTCGGGGCTGCG |
NGG |
2 |
0.1793 |
Tier II |
| 5 |
NC_000073.6 |
7 |
63901553 |
+ |
Klf13 |
NNNCCTCGCTCGGGGCTGGG |
NGG |
2 |
0.1625 |
Tier II |
| 6 |
NC_000069.6 |
3 |
67952635 |
- |
Iqcj |
NNNCCTCCCTGGGGACTGTG |
NGG |
2 |
0.1538 |
Tier II |
| 7 |
NC_000069.6 |
3 |
67952635 |
- |
Iqschfp |
NNNCCTCCCTGGGGACTGTG |
NGG |
2 |
0.1538 |
Tier II |
| 8 |
NC_000077.6 |
11 |
119344764 |
+ |
Sgsh |
NNNCCTGGCTTGGGACTGTG |
NGG |
2 |
0.1448 |
Tier II |
| 9 |
NC_000077.6 |
11 |
119344764 |
+ |
Card14 |
NNNCCTGGCTTGGGACTGTG |
NGG |
2 |
0.1448 |
Tier II |
| 10 |
NC_000081.6 |
15 |
87624636 |
- |
Tafa5 |
NNNCCTCGCCCGGGACGGTG |
NGG |
2 |
0.0627 |
Tier II |
| 11 |
NC_000075.6 |
9 |
20713857 |
+ |
Olfm2 |
NNNCCTCGCTCGGGACAGTG |
NGA |
2 |
0.037 |
Tier II |
Other clones with same target sequence:
(none)
