Construct: sgRNA BRDN0001145334
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGGGTGCCATAATGACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PACSIN1 (29993)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77155
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
34528687 |
+ |
PACSIN1 |
NNNGGGTGCCATAATGACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
99044599 |
+ |
COL15A1 |
NNNGGGTGCCATCCTGACAG |
NGG |
2 |
0.0451 |
Tier I |
| 3 |
NC_000004.12 |
4 |
76335520 |
- |
CCDC158 |
NNNAGGTGCCACAATGACAG |
NGG |
2 |
0.8526 |
Tier II |
| 4 |
NC_000001.11 |
1 |
52381779 |
+ |
ORC1 |
NNNGGGAGCCAAAATGACAG |
NGG |
2 |
0.7 |
Tier II |
| 5 |
NC_000006.12 |
6 |
118827524 |
+ |
MCM9 |
NNNGGTTGCTATAATGACAG |
NGG |
2 |
0.6275 |
Tier II |
| 6 |
NC_000002.12 |
2 |
177216428 |
+ |
HNRNPA3 |
NNNGGGTTACATAATGACAG |
NGG |
2 |
0.5357 |
Tier II |
| 7 |
NC_000013.11 |
13 |
21394160 |
- |
ZDHHC20 |
NNNGGGGGCCAAAATGACAG |
NGG |
2 |
0.4706 |
Tier II |
| 8 |
NC_000016.10 |
16 |
296929 |
+ |
AXIN1 |
NNNGGGTGCCACAGTGACAG |
NGG |
2 |
0.4421 |
Tier II |
| 9 |
NC_000013.11 |
13 |
93280722 |
- |
GPC6 |
NNNGGGTGCCAAAATGACTG |
NGG |
2 |
0.4308 |
Tier II |
| 10 |
NC_000011.10 |
11 |
16337607 |
+ |
SOX6 |
NNNGGGTGCCATGATGAAAG |
NGG |
2 |
0.3512 |
Tier II |
| 11 |
NC_000022.11 |
22 |
20741171 |
+ |
PI4KA |
NNNGGGTGTCATAATGGCAG |
NGG |
2 |
0.1544 |
Tier II |
| 12 |
NC_000008.11 |
8 |
140275463 |
- |
TRAPPC9 |
NNNTGGTGCCATCATGACAG |
NGG |
2 |
0.0766 |
Tier II |
| 13 |
NC_000001.11 |
1 |
66311840 |
+ |
PDE4B |
NNNGGGTGCCCTAATGCCAG |
NGG |
2 |
0.0706 |
Tier II |
| 14 |
NC_000009.12 |
9 |
125523852 |
- |
MAPKAP1 |
NNNGGGGGCCATAAGGACAG |
NGG |
2 |
0.0294 |
Tier II |
| 15 |
NC_000022.11 |
22 |
42562882 |
+ |
SERHL2 |
NNNGGGTGCCCTAAGGACAG |
NGG |
2 |
0.02 |
Tier II |
| 16 |
NC_000007.14 |
7 |
22458206 |
+ |
STEAP1B |
NNNGGGTGCCAAAATCACAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000001.11 |
1 |
53331786 |
- |
LRP8-DT |
NNNGGGTGCCATATTGACAG |
NAG |
2 |
0.1383 |
Tier III |
| 18 |
NC_000003.12 |
3 |
192734460 |
- |
LOC107986056 |
NNNGGGTGCCATAATGAGAT |
NGG |
2 |
0.0933 |
Tier III |
| 19 |
NC_000008.11 |
8 |
140275463 |
- |
LOC105375779 |
NNNTGGTGCCATCATGACAG |
NGG |
2 |
0.0766 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
88854111 |
+ |
Akap1 |
NNNGCCTGCCATAATGACAG |
NGG |
2 |
0.5357 |
Tier II |
| 2 |
NC_000076.6 |
10 |
118888627 |
- |
Dyrk2 |
NNNGGGTGCCACATTGACAG |
NGG |
2 |
0.5053 |
Tier II |
| 3 |
NC_000071.6 |
5 |
72138226 |
- |
Gabrb1 |
NNNCAGTGCCATAATGACAG |
NGG |
2 |
0.4588 |
Tier II |
| 4 |
NC_000067.6 |
1 |
179367380 |
+ |
Smyd3 |
NNNGGATGCCATAATGACAC |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000069.6 |
3 |
114960423 |
+ |
Olfm3 |
NNNTGGTGACATAATGACAG |
NGG |
2 |
0.3117 |
Tier II |
| 6 |
NC_000068.7 |
2 |
139691102 |
+ |
Ism1 |
NNNGTGTGACATAATGACAG |
NGG |
2 |
0.2571 |
Tier II |
| 7 |
NC_000068.7 |
2 |
35768988 |
+ |
Ttll11 |
NNNGGGTGCCAAAATGACAG |
NAG |
2 |
0.2074 |
Tier II |
| 8 |
NC_000076.6 |
10 |
128573112 |
- |
Erbb3 |
NNNGGATGCCATAATGAGAG |
NGG |
2 |
0.1333 |
Tier II |
| 9 |
NC_000083.6 |
17 |
56824662 |
+ |
Rfx2 |
NNNGTGTGCCATCATGACAG |
NGG |
2 |
0.0632 |
Tier II |
| 10 |
NC_000084.6 |
18 |
62649251 |
+ |
Spink10 |
NNNGGGAGCCATAATGACAG |
NGA |
2 |
0.0608 |
Tier II |
| 11 |
NC_000084.6 |
18 |
62649251 |
+ |
Spink13 |
NNNGGGAGCCATAATGACAG |
NGA |
2 |
0.0608 |
Tier II |
| 12 |
NC_000071.6 |
5 |
43267599 |
+ |
Cpeb2 |
NNNGTGTGCCATAATGGCAG |
NGG |
2 |
0.0529 |
Tier II |
| 13 |
NC_000071.6 |
5 |
114653274 |
+ |
Trpv4 |
NNNGGGTGTCATAATGACAG |
NGT |
2 |
0.0141 |
Tier II |
| 14 |
NC_000077.6 |
11 |
88854111 |
+ |
4930556N13Rik |
NNNGCCTGCCATAATGACAG |
NGG |
2 |
0.5357 |
Tier III |
| 15 |
NC_000076.6 |
10 |
118888627 |
- |
Gm26976 |
NNNGGGTGCCACATTGACAG |
NGG |
2 |
0.5053 |
Tier III |
| 16 |
NC_000072.6 |
6 |
97628534 |
- |
Gm32247 |
NNNGGGTGCCAAAAGGACAG |
NGG |
2 |
0.04 |
Tier III |
| 17 |
NC_000068.7 |
2 |
132151595 |
- |
Gm14051 |
NNNGGGTGCCAGAAGGACAG |
NGG |
2 |
0.025 |
Tier III |
| 18 |
NC_000067.6 |
1 |
155115896 |
+ |
Gm41981 |
NNNGGGTGACATAATGACAG |
NGT |
2 |
0.0138 |
Tier III |
Other clones with same target sequence:
(none)
