Construct: sgRNA BRDN0001145341
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAATGACGTGGCATCACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TYK2 (7297)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77248
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
10366548 |
- |
TYK2 |
NNNATGACGTGGCATCACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
136303257 |
- |
CCDC187 |
NNNATGACGTGGCAGCCCTG |
NGG |
2 |
0.0088 |
Tier I |
| 3 |
NC_000008.11 |
8 |
1091583 |
- |
DLGAP2 |
NNNATGATATGGCATCACTG |
NGG |
2 |
0.5625 |
Tier II |
| 4 |
NC_000009.12 |
9 |
128247553 |
- |
DNM1 |
NNNATGACTTGGCATCACTG |
NGG |
1 |
0.5333 |
Tier II |
| 5 |
NC_000016.10 |
16 |
73665747 |
+ |
ZFHX3 |
NNNATGATTTGGCATCACTG |
NGG |
2 |
0.4667 |
Tier II |
| 6 |
NC_000008.11 |
8 |
38285874 |
- |
NSD3 |
NNNGTGACGTGGAATCACTG |
NGG |
2 |
0.2404 |
Tier II |
| 7 |
NC_000023.11 |
X |
67713753 |
+ |
AR |
NNNATGACTTGGGATCACTG |
NGG |
2 |
0.0727 |
Tier II |
| 8 |
NC_000018.10 |
18 |
23979420 |
- |
LINC02958 |
NNNATGACATGGCATCACTT |
NGG |
2 |
0.45 |
Tier III |
| 9 |
NC_000007.14 |
7 |
143493784 |
+ |
EPHA1-AS1 |
NNNATGCTGTGGCATCACTG |
NGG |
2 |
0.3828 |
Tier III |
| 10 |
NC_000007.14 |
7 |
143493784 |
+ |
LOC105375547 |
NNNATGCTGTGGCATCACTG |
NGG |
2 |
0.3828 |
Tier III |
| 11 |
NC_000010.11 |
10 |
101108025 |
+ |
TLX1NB |
NNNATGCCGTGGCACCACTG |
NGG |
2 |
0.1193 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
90797685 |
- |
Cxcl15 |
NNNATGATGTGGCTTCACTG |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000085.6 |
19 |
22345578 |
+ |
Trpm3 |
NNNATCACATGGCATCACTG |
NGG |
2 |
0.4383 |
Tier II |
| 3 |
NC_000083.6 |
17 |
28413218 |
+ |
Fkbp5 |
NNNATGACCTGGCATCACTT |
NGG |
2 |
0.3769 |
Tier II |
| 4 |
NC_000073.6 |
7 |
133768573 |
- |
Dhx32 |
NNNATCACGTGGCATCACAG |
NGG |
2 |
0.1948 |
Tier II |
| 5 |
NC_000068.7 |
2 |
73009912 |
+ |
Gm39850 |
NNNATAACGTGGCATCACTG |
NGA |
2 |
0.0694 |
Tier III |
Other clones with same target sequence:
(none)
