Construct: sgRNA BRDN0001145345
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAAATGATTAAGTTGACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRAF (673)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76479
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
140850182 |
- |
BRAF |
NNNAATGATTAAGTTGACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
21301191 |
- |
SLCO1A2 |
NNNAATGTTTATGTTGACAC |
NGG |
2 |
0.1429 |
Tier I |
| 3 |
NC_000007.14 |
7 |
92704719 |
- |
CDK6 |
NNNAATGATTAAATTGACAC |
NGG |
1 |
0.9231 |
Tier II |
| 4 |
NC_000010.11 |
10 |
51807488 |
+ |
PRKG1 |
NNNAAAGATTAAGATGACAC |
NGG |
2 |
0.5365 |
Tier II |
| 5 |
NC_000002.12 |
2 |
173038188 |
- |
RAPGEF4 |
NNNAATTATTAAATTGACAC |
NGG |
2 |
0.5275 |
Tier II |
| 6 |
NC_000001.11 |
1 |
93712202 |
- |
BCAR3 |
NNNAATGATCAAGTTGATAC |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000005.10 |
5 |
156621642 |
+ |
SGCD |
NNNAGTGATTAAGTTGAGAC |
NGG |
2 |
0.096 |
Tier II |
| 8 |
NC_000006.12 |
6 |
39618746 |
- |
KIF6 |
NNNAATGCTTAAGTTGAGAC |
NGG |
2 |
0.0571 |
Tier II |
| 9 |
NC_000010.11 |
10 |
123785599 |
- |
CPXM2 |
NNNCATGATTAAGTTGTCAC |
NGG |
2 |
0.0471 |
Tier II |
| 10 |
NC_000016.10 |
16 |
69893151 |
- |
WWP2 |
NNNACTGATTAAGTTGACAC |
NTG |
2 |
0.0195 |
Tier II |
| 11 |
NC_000003.12 |
3 |
143727849 |
- |
SLC9A9 |
NNNAATGATTAAGTTGACAG |
NGA |
2 |
0.0041 |
Tier II |
| 12 |
NC_000018.10 |
18 |
6833749 |
- |
ARHGAP28 |
NNNAATGACTAAGTTCACAC |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000005.10 |
5 |
100405830 |
- |
LOC105379100 |
NNNAATGCTTAAGTTAACAC |
NGG |
2 |
0.4286 |
Tier III |
| 14 |
NC_000011.10 |
11 |
77643438 |
- |
LOC107984369 |
NNNAATGATTAAGGTCACAC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
39688315 |
- |
Braf |
NNNAATGATTAAGTTGACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
50603925 |
+ |
Nell1 |
NNNAATGAAAAAGTTGACAC |
NGG |
2 |
0.7959 |
Tier II |
| 3 |
NC_000074.6 |
8 |
17387773 |
- |
C030002A05Rik |
NNNGATGTTTAAGTTGACAC |
NGG |
2 |
0.2679 |
Tier II |
| 4 |
NC_000074.6 |
8 |
17387773 |
- |
Csmd1 |
NNNGATGTTTAAGTTGACAC |
NGG |
2 |
0.2679 |
Tier II |
| 5 |
NC_000085.6 |
19 |
57890475 |
- |
Atrnl1 |
NNNAAAGATTAAGTTGACAT |
NGG |
2 |
0.26 |
Tier II |
| 6 |
NC_000069.6 |
3 |
132669376 |
- |
Aimp1 |
NNNAGTGATTAATTTGACAC |
NGG |
2 |
0.216 |
Tier II |
| 7 |
NC_000078.6 |
12 |
30299495 |
- |
Sntg2 |
NNNAATGTTTAAGTTGACAT |
NGG |
2 |
0.1286 |
Tier II |
| 8 |
NC_000086.7 |
X |
109098323 |
+ |
Sh3bgrl |
NNNAATGATTAAGTTGTCTC |
NGG |
2 |
0.0718 |
Tier II |
| 9 |
NC_000079.6 |
13 |
18227481 |
- |
Pou6f2 |
NNNAATGATCAAGTTGACAG |
NGG |
2 |
0.0314 |
Tier II |
| 10 |
NC_000086.7 |
X |
137027239 |
+ |
Slc25a53 |
NNNAATGATTAAGTTGACAG |
NGT |
2 |
0.0009 |
Tier II |
| 11 |
NC_000074.6 |
8 |
99575288 |
- |
A330008L17Rik |
NNNAATAATTAAATTGACAC |
NGG |
2 |
0.9231 |
Tier III |
| 12 |
NC_000068.7 |
2 |
156382138 |
- |
Gm14170 |
NNNAATAGTTAAGTTGACAC |
NGG |
2 |
0.7333 |
Tier III |
Other clones with same target sequence:
(none)
