Construct: sgRNA BRDN0001145354
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCATAAGAAATGCAGCGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME7 (29922)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76495
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
169298590 |
- |
NME7 |
NNNATAAGAAATGCAGCGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
105042527 |
+ |
KMT2E |
NNNATAAGAAATGCAGCTCA |
NGG |
1 |
0.6667 |
Tier II |
| 3 |
NC_000017.11 |
17 |
2070726 |
+ |
SMG6 |
NNNATGAGAAATGCAGCTCA |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000023.11 |
X |
38378343 |
- |
OTC |
NNNATAAGAAATACAGAGCA |
NGG |
2 |
0.4308 |
Tier II |
| 5 |
NC_000005.10 |
5 |
137374737 |
- |
SPOCK1 |
NNNTTAAGAAATGCAGTGCA |
NGG |
2 |
0.297 |
Tier II |
| 6 |
NC_000014.9 |
14 |
33491211 |
- |
NPAS3 |
NNNATAAGAAATGCAGCAAA |
NGG |
2 |
0.2967 |
Tier II |
| 7 |
NC_000023.11 |
X |
108403569 |
- |
COL4A6 |
NNNATAAGAAATGCAGTGAA |
NGG |
2 |
0.2 |
Tier II |
| 8 |
NC_000010.11 |
10 |
48590841 |
+ |
ARHGAP22 |
NNNAGAAGAAATTCAGCGCA |
NGG |
2 |
0.192 |
Tier II |
| 9 |
NC_000008.11 |
8 |
99812565 |
- |
VPS13B |
NNNATAAGAAATGCAGAGCA |
NAG |
2 |
0.121 |
Tier II |
| 10 |
NC_000007.14 |
7 |
70305210 |
- |
AUTS2 |
NNNATAAGAAATGCAGTGCA |
NAG |
2 |
0.121 |
Tier II |
| 11 |
NC_000004.12 |
4 |
151487174 |
- |
FHIP1A |
NNNATAAGAAATACAGGGCA |
NGG |
2 |
0.0543 |
Tier II |
| 12 |
NC_000002.12 |
2 |
38063909 |
- |
RMDN2 |
NNNATAAGAAATGCAGCACA |
NTG |
2 |
0.027 |
Tier II |
| 13 |
NC_000021.9 |
21 |
33445525 |
+ |
TMEM50B |
NNNATAAGAAATGCCGGGCA |
NGG |
2 |
0.016 |
Tier II |
| 14 |
NC_000006.12 |
6 |
97118736 |
- |
KLHL32 |
NNNATAAGAAATGCAGCACA |
NGT |
2 |
0.0112 |
Tier II |
| 15 |
NC_000011.10 |
11 |
70921606 |
+ |
SHANK2 |
NNNATAAGAAATGGAGGGCA |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000022.11 |
22 |
20964227 |
- |
LINC01637 |
NNNATAGGAAATGCAGCCCA |
NGG |
2 |
0.3361 |
Tier III |
| 17 |
NC_000003.12 |
3 |
155760928 |
- |
RPL7AP24 |
NNNATAAGAAATGCAGATCA |
NGG |
2 |
0.3111 |
Tier III |
| 18 |
NC_000013.11 |
13 |
91208831 |
+ |
LINC00379 |
NNNATAAGAAAGGCAGAGCA |
NGG |
2 |
0.2333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
66202503 |
+ |
Dapk2 |
NNNAGAGGAAATGCAGCGCA |
NGG |
2 |
0.4518 |
Tier II |
| 2 |
NC_000076.6 |
10 |
70157808 |
+ |
Ccdc6 |
NNNATAAGAAATGCAGTGCT |
NGG |
2 |
0.28 |
Tier II |
| 3 |
NC_000075.6 |
9 |
13623528 |
+ |
Maml2 |
NNNCTAAGAAATGCAGCTCA |
NGG |
2 |
0.2353 |
Tier II |
| 4 |
NC_000068.7 |
2 |
19203236 |
+ |
Armc3 |
NNNCTAAGAAATGCAGCCCA |
NGG |
2 |
0.1681 |
Tier II |
| 5 |
NC_000081.6 |
15 |
10347962 |
+ |
Prlr |
NNNATAAGAAATGAAGCCCA |
NGG |
2 |
0.1667 |
Tier II |
| 6 |
NC_000081.6 |
15 |
103463313 |
+ |
Nckap1l |
NNNATAAGAAATGCAACGGA |
NGG |
2 |
0.125 |
Tier II |
| 7 |
NC_000083.6 |
17 |
69078108 |
- |
Epb41l3 |
NNNATAAGAAATGCAGGGTA |
NGG |
2 |
0.0271 |
Tier II |
| 8 |
NC_000076.6 |
10 |
95495975 |
+ |
Mrpl42 |
NNNATAAGAAATGCTGGGCA |
NGG |
2 |
0.0118 |
Tier II |
| 9 |
NC_000067.6 |
1 |
186917378 |
+ |
Gm39732 |
NNNACAAGAAATGCAGCTCA |
NGG |
2 |
0.6667 |
Tier III |
| 10 |
NC_000068.7 |
2 |
117884764 |
- |
4930412B13Rik |
NNNATAAGAAATGCAGCCGA |
NGG |
2 |
0.0595 |
Tier III |
| 11 |
NC_000068.7 |
2 |
117884764 |
- |
Gm13986 |
NNNATAAGAAATGCAGCCGA |
NGG |
2 |
0.0595 |
Tier III |
Other clones with same target sequence:
(none)
