Construct: sgRNA BRDN0001145357
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGGTCCCAGCGAGCCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PINK1 (65018)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78036
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
20639953 |
+ |
PINK1 |
NNNGGTCCCAGCGAGCCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
45161773 |
- |
NKPD1 |
NNNGGCCCCAGAGAGCCGAG |
NGG |
2 |
0.6494 |
Tier II |
| 3 |
NC_000016.10 |
16 |
67110363 |
- |
PHAF1 |
NNNGAGCCCAGCGAGCCGAG |
NGG |
2 |
0.4952 |
Tier II |
| 4 |
NC_000002.12 |
2 |
108912115 |
- |
RANBP2 |
NNNGGTCTCAGCGAGCCGTG |
NGG |
2 |
0.4712 |
Tier II |
| 5 |
NC_000002.12 |
2 |
108912115 |
- |
EDAR |
NNNGGTCTCAGCGAGCCGTG |
NGG |
2 |
0.4712 |
Tier II |
| 6 |
NC_000001.11 |
1 |
27405668 |
- |
WASF2 |
NNNGGACCCAGTGAGCCGAG |
NGG |
2 |
0.4667 |
Tier II |
| 7 |
NC_000020.11 |
20 |
49907740 |
- |
SPATA2 |
NNNGGTCCCAGCAAGCTGAG |
NGG |
2 |
0.4308 |
Tier II |
| 8 |
NC_000004.12 |
4 |
86453100 |
- |
MAPK10 |
NNNGGACCCAGCGAGCCCAG |
NGG |
2 |
0.4127 |
Tier II |
| 9 |
NC_000015.10 |
15 |
74854318 |
- |
SCAMP2 |
NNNGGACCCAGCGAGCCCAG |
NGG |
2 |
0.4127 |
Tier II |
| 10 |
NC_000008.11 |
8 |
144234962 |
- |
MROH1 |
NNNGGTCACAGTGAGCCGAG |
NGG |
2 |
0.35 |
Tier II |
| 11 |
NC_000009.12 |
9 |
132735138 |
+ |
AK8 |
NNNGGTCCCAGAGAGCTGAG |
NGG |
2 |
0.3333 |
Tier II |
| 12 |
NC_000010.11 |
10 |
110920057 |
- |
SHOC2 |
NNNGGGCCCAGGGAGCCGAG |
NGG |
2 |
0.254 |
Tier II |
| 13 |
NC_000019.10 |
19 |
39172688 |
+ |
PAK4 |
NNNGGTCCCACCGAGCCCAG |
NGG |
2 |
0.2041 |
Tier II |
| 14 |
NC_000019.10 |
19 |
14439068 |
- |
PKN1 |
NNNGGTCCCAGCCAGCCCAG |
NGG |
2 |
0.2005 |
Tier II |
| 15 |
NC_000007.14 |
7 |
13970154 |
+ |
ETV1 |
NNNGGTCCCAGCTAGCCGGG |
NGG |
2 |
0.1125 |
Tier II |
| 16 |
NC_000011.10 |
11 |
60992005 |
- |
CD6 |
NNNGGTCCCAGCTAGTCGAG |
NGG |
2 |
0.0923 |
Tier II |
| 17 |
NC_000008.11 |
8 |
18875041 |
- |
PSD3 |
NNNGGTCCCAGCTACCCGAG |
NGG |
2 |
0.0818 |
Tier II |
| 18 |
NC_000015.10 |
15 |
43142795 |
- |
TMEM62 |
NNNGGTCCCAGCTACCCGAG |
NGG |
2 |
0.0818 |
Tier II |
| 19 |
NC_000019.10 |
19 |
6778628 |
+ |
VAV1 |
NNNGGTCCCAGCTACCCGAG |
NGG |
2 |
0.0818 |
Tier II |
| 20 |
NC_000002.12 |
2 |
105399124 |
- |
FHL2 |
NNNGGTGCCAGCGAGCCGAG |
NCG |
2 |
0.0504 |
Tier II |
| 21 |
NC_000010.11 |
10 |
71625239 |
+ |
CDH23 |
NNNGGTCCCACCGAGCGGAG |
NGG |
2 |
0.0252 |
Tier II |
| 22 |
NC_000002.12 |
2 |
206691362 |
+ |
DYTN |
NNNGGTCGCAGCGAGCCGAG |
NTG |
2 |
0.025 |
Tier II |
| 23 |
NC_000012.12 |
12 |
3321365 |
- |
LOC100128253 |
NNNGGTCCCCGAGAGCCGAG |
NGG |
2 |
0.2381 |
Tier III |
| 24 |
NC_000012.12 |
12 |
3321365 |
- |
LINC02827 |
NNNGGTCCCCGAGAGCCGAG |
NGG |
2 |
0.2381 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
114130594 |
+ |
Ung |
NNNGGTCCCAGGGAGCGGAG |
NGG |
2 |
0.0261 |
Tier I |
| 2 |
NC_000076.6 |
10 |
86827280 |
- |
Nt5dc3 |
NNNTGTCCCAGAGAGCCGAG |
NGG |
2 |
0.2597 |
Tier II |
| 3 |
NC_000070.6 |
4 |
70378448 |
+ |
Cdk5rap2 |
NNNGGTCCCACCGAGCAGAG |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000070.6 |
4 |
66034822 |
+ |
Astn2 |
NNNGGTCCCAGAGATCCGAG |
NGG |
2 |
0.102 |
Tier II |
| 5 |
NC_000071.6 |
5 |
114130594 |
+ |
Alkbh2 |
NNNGGTCCCAGGGAGCGGAG |
NGG |
2 |
0.0261 |
Tier II |
| 6 |
NC_000070.6 |
4 |
132048283 |
- |
Epb41 |
NNNGGTCCCAGCGAGCCAAG |
NGT |
2 |
0.0112 |
Tier II |
Other clones with same target sequence:
(none)
