Construct: sgRNA BRDN0001145363
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAAGGACCTGTACGTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKG1 (5592)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77007
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
50991586 |
- |
PRKG1 |
NNNAAGGACCTGTACGTCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
133043338 |
- |
USP26 |
NNNAAGGACCTGTGTGTCTG |
NGG |
2 |
0.0311 |
Tier II |
| 3 |
NC_000020.11 |
20 |
13927667 |
+ |
SEL1L2 |
NNNAAGGACCGGTAGGTCTG |
NGG |
2 |
0.02 |
Tier II |
| 4 |
NC_000011.10 |
11 |
77438141 |
+ |
PAK1 |
NNNAAGGACCTGTCCTTCTG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
98072653 |
+ |
Notch2 |
NNNAAGCACCTGTACATCTG |
NGG |
2 |
0.6875 |
Tier I |
| 2 |
NC_000085.6 |
19 |
31764616 |
+ |
Prkg1 |
NNNAAGGACCTGTAGGTCTG |
NGG |
1 |
0.05 |
Tier I |
| 3 |
NC_000073.6 |
7 |
143560820 |
+ |
Cars |
NNNAGGTACCTGTACGTCTG |
NGG |
2 |
0.4114 |
Tier II |
| 4 |
NC_000080.6 |
14 |
56692192 |
- |
Mphosph8 |
NNNAAGGACCTGTACGACTC |
NGG |
2 |
0.2286 |
Tier II |
| 5 |
NC_000074.6 |
8 |
117397999 |
+ |
Cmip |
NNNAAGGACCCGTACGTCAG |
NGG |
2 |
0.1905 |
Tier II |
| 6 |
NC_000068.7 |
2 |
121596363 |
+ |
Frmd5 |
NNNCAGGACCTGTGCGTCTG |
NGG |
2 |
0.1647 |
Tier II |
| 7 |
NC_000069.6 |
3 |
86692168 |
- |
Lrba |
NNNAAGGACCTGTATGTGTG |
NGG |
2 |
0.0089 |
Tier II |
Other clones with same target sequence:
(none)
