Construct: sgRNA BRDN0001145364
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTATTCAGAAGGATCCGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCLK2 (166614)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75698
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
150102694 |
- |
DCLK2 |
NNNATTCAGAAGGATCCGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
59609581 |
- |
SLC16A7 |
NNNATTCAGAAGGATCCACA |
NGG |
1 |
0.6923 |
Tier II |
| 3 |
NC_000016.10 |
16 |
80491 |
- |
MPG |
NNNTTTTAGAAGGATCCGCA |
NGG |
2 |
0.517 |
Tier II |
| 4 |
NC_000002.12 |
2 |
109035101 |
+ |
RANBP2 |
NNNATCCAGAAGGATCAGCA |
NGG |
2 |
0.4242 |
Tier II |
| 5 |
NC_000010.11 |
10 |
131908333 |
- |
PPP2R2D |
NNNATTTAGAAGGATCAGCA |
NGG |
2 |
0.3792 |
Tier II |
| 6 |
NC_000002.12 |
2 |
73894844 |
+ |
ACTG2 |
NNNATTCAGAGGGGTCCGCA |
NGG |
2 |
0.3033 |
Tier II |
| 7 |
NC_000003.12 |
3 |
25160789 |
+ |
RARB |
NNNTTTCAGAAGGATCTGCA |
NGG |
2 |
0.297 |
Tier II |
| 8 |
NC_000004.12 |
4 |
168978219 |
- |
CBR4 |
NNNATTCAGAAGGAACAGCA |
NGG |
2 |
0.2702 |
Tier II |
| 9 |
NC_000012.12 |
12 |
26065566 |
- |
RASSF8 |
NNNATGGAGAAGGATCCGCA |
NGG |
2 |
0.2689 |
Tier II |
| 10 |
NC_000001.11 |
1 |
38854515 |
+ |
RRAGC |
NNNAATCAGAAGGATCCCCA |
NGG |
2 |
0.2381 |
Tier II |
| 11 |
NC_000018.10 |
18 |
57012487 |
+ |
WDR7 |
NNNATTCAGAAGGGTCTGCA |
NGG |
2 |
0.2178 |
Tier II |
| 12 |
NC_000003.12 |
3 |
161347970 |
- |
SPTSSB |
NNNATTCAGAAGGAGCCACA |
NGG |
2 |
0.0346 |
Tier II |
| 13 |
NC_000012.12 |
12 |
90947280 |
- |
LINC00615 |
NNNATTCAGAAGGATTCCCA |
NGG |
2 |
0.1465 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
86906004 |
+ |
Dclk2 |
NNNATTCAGAAGGATCCGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000085.6 |
19 |
26681971 |
- |
Smarca2 |
NNNAGTCAGAAGGATCCGCC |
NGG |
2 |
0.1455 |
Tier I |
| 3 |
NC_000068.7 |
2 |
25585085 |
+ |
Rabl6 |
NNNATTCAGAAAGATCAGCA |
NGG |
2 |
0.4356 |
Tier II |
| 4 |
NC_000078.6 |
12 |
89795404 |
+ |
Nrxn3 |
NNNATTCAGAGGGATCCTCA |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000074.6 |
8 |
13197000 |
- |
Grtp1 |
NNNATTCAGAAGGAACCTCA |
NGG |
2 |
0.386 |
Tier II |
| 6 |
NC_000078.6 |
12 |
83150290 |
- |
Rgs6 |
NNNATGCAGAAGGATCCCCA |
NGG |
2 |
0.2721 |
Tier II |
| 7 |
NC_000083.6 |
17 |
58308335 |
+ |
Cntnap5c |
NNNATTCAGAAGGAACTGCA |
NGG |
2 |
0.2702 |
Tier II |
| 8 |
NC_000073.6 |
7 |
68175175 |
- |
Igf1r |
NNNATGCAGAAGGATTCGCA |
NGG |
2 |
0.1758 |
Tier II |
| 9 |
NC_000074.6 |
8 |
95027341 |
- |
Adgrg3 |
NNNATTCAGAAGTATCCGCA |
NAG |
2 |
0.0778 |
Tier II |
| 10 |
NC_000077.6 |
11 |
55527844 |
+ |
Glra1 |
NNNATTCAGAAGGATGTGCA |
NGG |
2 |
0.0718 |
Tier II |
| 11 |
NC_000077.6 |
11 |
89074589 |
+ |
Gm525 |
NNNATTCAGAAGGATCCGAA |
NGT |
2 |
0.0069 |
Tier II |
| 12 |
NC_000078.6 |
12 |
83150290 |
- |
Gm51988 |
NNNATGCAGAAGGATCCCCA |
NGG |
2 |
0.2721 |
Tier III |
| 13 |
NC_000077.6 |
11 |
55527844 |
+ |
Gm12236 |
NNNATTCAGAAGGATGTGCA |
NGG |
2 |
0.0718 |
Tier III |
| 14 |
NC_000068.7 |
2 |
12082966 |
- |
Gm13310 |
NNNATTCAGAAGGAGCCACA |
NGG |
2 |
0.0346 |
Tier III |
| 15 |
NC_000081.6 |
15 |
88242325 |
+ |
4930445N06Rik |
NNNATTCAGAAGGCTCCGCA |
NGA |
2 |
0.0149 |
Tier III |
Other clones with same target sequence:
(none)
