Construct: sgRNA BRDN0001145376

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTGTGATAAAAATGACCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NRBP1 (29959)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77016

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 27434471 - NRBP1 NNNGTGATAAAAATGACCTG NGG 0 1.0 Tier I
2 NC_000005.10 5 139914181 + NRG2 NNNATAATAAAAATGACCTG NGG 2 0.9 Tier II
3 NC_000007.14 7 98857374 - TMEM130 NNNGTGACAAAAGTGACCTG NGG 2 0.6522 Tier II
4 NC_000012.12 12 70583224 + PTPRB NNNATGATAAAAATGACCTT NGG 2 0.63 Tier II
5 NC_000007.14 7 26736306 + SKAP2 NNNGTGATGAAAATGACCTT NGG 2 0.4667 Tier II
6 NC_000001.11 1 162647795 - DDR2 NNNGTGATGAAAGTGACCTG NGG 2 0.4348 Tier II
7 NC_000003.12 3 114986207 - ZBTB20 NNNGTGATAAGAGTGACCTG NGG 2 0.4239 Tier II
8 NC_000005.10 5 96470956 + CAST NNNCTGATAAAAATGACCTT NGG 2 0.3706 Tier II
9 NC_000006.12 6 118507560 - CEP85L NNNGTGATATCAATGACCTG NGG 2 0.3529 Tier II
10 NC_000008.11 8 47284340 - SPIDR NNNGTGATAAAAATGAACTT NGG 2 0.3267 Tier II
11 NC_000014.9 14 24192629 - TM9SF1 NNNGTGATAAAAATGAATTG NGG 2 0.3 Tier II
12 NC_000002.12 2 62071333 - COMMD1 NNNGTGATAAAAGTGACCTC NGG 2 0.2795 Tier II
13 NC_000003.12 3 60260837 + FHIT NNNGTGATAAAAGTGACCTC NGG 2 0.2795 Tier II
14 NC_000012.12 12 513513 - B4GALNT3 NNNGTGATAAAAGTGACCTC NGG 2 0.2795 Tier II
15 NC_000016.10 16 12141763 - SNX29 NNNGTGATAAAAGTGACCTC NGG 2 0.2795 Tier II
16 NC_000006.12 6 110334284 - METTL24 NNNGTGATAAAAATGACTTC NGG 2 0.2755 Tier II
17 NC_000009.12 9 72929922 - ALDH1A1 NNNGTAATAAAAATGACCTG NAG 2 0.2593 Tier II
18 NC_000007.14 7 50643971 + GRB10 NNNGTGAAAATAATGACCTG NGG 2 0.2462 Tier II
19 NC_000009.12 9 20773449 - FOCAD NNNATGATAAAAATCACCTG NGG 2 0.2455 Tier II
20 NC_000014.9 14 37401050 + MIPOL1 NNNGTGATAAAAATGACCAT NGG 2 0.2 Tier II
21 NC_000017.11 17 51846707 + CA10 NNNTTGATAAAAATGACATG NGG 2 0.1958 Tier II
22 NC_000008.11 8 139661579 + KCNK9 NNNGTGATAAGAATCACCTG NGG 2 0.1773 Tier II
23 NC_000010.11 10 10531346 - CELF2 NNNGTGATTAAAATCACCTG NGG 2 0.1636 Tier II
24 NC_000004.12 4 93752997 + GRID2 NNNGTGATAAGAATGGCCTG NGG 2 0.125 Tier II
25 NC_000013.11 13 75457806 - TBC1D4 NNNTTGATAAAAATCACCTG NGG 2 0.0992 Tier II
26 NC_000001.11 1 111858672 - KCND3 NNNGTGCTAAAACTGACCTG NGG 2 0.0921 Tier II
27 NC_000006.12 6 143158097 - AIG1 NNNGTGATAAAAATGAGCTG NGG 1 0.0588 Tier II
28 NC_000007.14 7 108125864 + LAMB4 NNNATGATAAAAATGACCTG NTG 2 0.0351 Tier II
29 NC_000010.11 10 27143091 + YME1L1 NNNGTGATAAAAATGGCCTG NTG 2 0.0075 Tier II
30 NC_000022.11 22 33169564 + LARGE1 NNNGTCATAAAAATGTCCTG NGG 2 0.0 Tier II
31 NC_000004.12 4 24197275 - PPARGC1A NNNGTGATAAAAATGACCTG NAA 2 0.0 Tier II
32 NC_000001.11 1 43187587 - CFAP57 NNNGTGATAAAAATGTCCTA NGG 2 0.0 Tier II
33 NC_000017.11 17 32466301 - PSMD11 NNNGTGATAAAAATGTTCTG NGG 2 0.0 Tier II
34 NC_000001.11 1 65156540 - AK4 NNNGTGTTAAAAAGGACCTG NGG 2 0.0 Tier II
35 NC_000007.14 7 25976504 - LOC105375199 NNNGTGAGAAAAATAACCTG NGG 2 0.6902 Tier III
36 NC_000007.14 7 47021475 + LOC105375268 NNNATGATAAAAAAGACCTG NGG 2 0.5571 Tier III
37 NC_000005.10 5 96470956 + LOC101929710 NNNCTGATAAAAATGACCTT NGG 2 0.3706 Tier III
38 NC_000005.10 5 96470956 + LOC102724070 NNNCTGATAAAAATGACCTT NGG 2 0.3706 Tier III
39 NC_000007.14 7 69576226 - LOC105375346 NNNGAGAGAAAAATGACCTG NGG 2 0.3667 Tier III
40 NC_000007.14 7 140962747 - LOC105375536 NNNGTGATGAAAATGAACTG NGG 2 0.3111 Tier III
41 NC_000003.12 3 60260837 + LOC107986015 NNNGTGATAAAAGTGACCTC NGG 2 0.2795 Tier III
42 NC_000006.12 6 7680706 - LOC105374906 NNNGTGATAAAAGTGACCTC NGG 2 0.2795 Tier III
43 NC_000018.10 18 64321503 - LINC01924 NNNGTGATAAAAGTGACCTC NGG 2 0.2795 Tier III
44 NC_000008.11 8 74636862 + MIR2052HG NNNGTGATAAAAATGAACTG NAG 2 0.121 Tier III
45 NC_000017.11 17 36415582 - LOC100420852 NNNGTGATAAAACTGACCTC NGG 2 0.0902 Tier III
46 NC_000006.12 6 143158097 - LOC124901416 NNNGTGATAAAAATGAGCTG NGG 1 0.0588 Tier III
47 NC_000011.10 11 39957963 - LOC105376637 NNNGTGATAAAAATTACCTG NGA 2 0.0099 Tier III
48 NC_000001.11 1 43187587 - LOC105378685 NNNGTGATAAAAATGTCCTA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 76090543 + Nrbp2 NNNGTGATGAAGATGACCTG NGG 2 0.4815 Tier I
2 NC_000082.6 16 90843125 + Eva1c NNNGTGACCAAAATGACCTG NGG 2 0.5714 Tier II
3 NC_000080.6 14 10082818 + Fhit NNNGTCAGAAAAATGACCTG NGG 2 0.5 Tier II
4 NC_000068.7 2 105397978 - Rcn1 NNNGTGATAAAAAAGACCTT NGG 2 0.4333 Tier II
5 NC_000071.6 5 88984878 - Slc4a4 NNNGTGATAAAGATGAACTG NGG 2 0.337 Tier II
6 NC_000083.6 17 33812959 + Kank3 NNNGTCTTAAAAATGACCTG NGG 2 0.2983 Tier II
7 NC_000083.6 17 52916088 + Kcnh8 NNNGTCTTAAAAATGACCTG NGG 2 0.2983 Tier II
8 NC_000082.6 16 17856573 - Dgcr2 NNNGTGAGAACAATGACCTG NGG 2 0.2933 Tier II
9 NC_000070.6 4 17927418 + Mmp16 NNNGTGATAAGAATGACCTC NGG 2 0.2786 Tier II
10 NC_000082.6 16 13338740 + Mrtfb NNNGTGATAAAAATGAAATG NGG 2 0.2513 Tier II
11 NC_000070.6 4 111194329 + Agbl4 NNNGTGATAAAAATGACTGG NGG 2 0.1607 Tier II
12 NC_000080.6 14 10225359 + Fhit NNNGTGCTAAAAATGACCTG NAG 2 0.1134 Tier II
13 NC_000067.6 1 62204927 - Pard3b NNNGTGATAAGAATTACCTG NGG 2 0.0929 Tier II
14 NC_000074.6 8 38498180 - Sgcz NNNGTGATAAAAATTACATG NGG 2 0.0769 Tier II
15 NC_000073.6 7 120599917 - Gm32916 NNNGTGATAAAAATGACCTT NCG 2 0.075 Tier II
16 NC_000068.7 2 34211532 + Pbx3 NNNATGATAAAAATGAGCTG NGG 2 0.0529 Tier II
17 NC_000069.6 3 142128893 - Bmpr1b NNNGTGACAAAAATGACCTG NGC 2 0.0222 Tier II
18 NC_000070.6 4 78144676 + Ptprd NNNGAGATAAAAATGACCTG NGC 2 0.0111 Tier II
19 NC_000081.6 15 64200783 - Asap1 NNNGTGATAAAAACGACCTG NGC 2 0.0063 Tier II
20 NC_000070.6 4 104584201 - Dab1 NNNGTGATAAAAAGGACCTG NGC 2 0.0 Tier II
21 NC_000081.6 15 82944275 - Tcf20 NNNGTGATAAAAATACCCTG NGG 2 0.0 Tier II
22 NC_000074.6 8 108768230 - Zfhx3 NNNGTGATAAAAATCTCCTG NGG 2 0.0 Tier II
23 NC_000067.6 1 62093704 - Pard3b NNNGTGATAAAAATGCCCTG NAG 2 0.0 Tier II
24 NC_000072.6 6 145751351 - Rassf8 NNNGTGATACAAATGTCCTG NGG 2 0.0 Tier II
25 NC_000081.6 15 57329487 - Slc22a22 NNNGTGATATAAATGTCCTG NGG 2 0.0 Tier II
26 NC_000071.6 5 127595018 - Slc15a4 NNNGTGATGAAAAGGACCTG NGG 2 0.0 Tier II
27 NC_000082.6 16 90843125 + Gm36363 NNNGTGACCAAAATGACCTG NGG 2 0.5714 Tier III
28 NC_000069.6 3 139168663 + Gm4862 NNNGTGATAAAAATGGCCTG NGG 1 0.1923 Tier III
29 NC_000068.7 2 75359803 - Gm39857 NNNGTGATAAAAAGGACTTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)