Construct: sgRNA BRDN0001145380

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGCTTATATCCATCTACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K9 (4293)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77308

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 70734485 + MAP3K9 NNNCTTATATCCATCTACCA NGG 0 1.0 Tier I
2 NC_000003.12 3 174196542 + NLGN1 NNNTTTATAACCATCTACCA NGG 2 0.6857 Tier II
3 NC_000015.10 15 98847957 - IGF1R NNNGTTATATCCATCTAACA NGG 2 0.2692 Tier II
4 NC_000012.12 12 57316320 - R3HDM2 NNNTTTATATCCATCTCCCA NGG 2 0.1412 Tier II
5 NC_000012.12 12 69884389 + MYRFL NNNCTTATAGCCATCTCCCA NGG 2 0.0882 Tier II
6 NC_000003.12 3 105492583 + ALCAM NNNCTTATTTCCATCTAGCA NGG 2 0.08 Tier II
7 NC_000003.12 3 10340035 - ATP2B2 NNNCTTATATCTATCTACCA NTG 2 0.021 Tier II
8 NC_000023.11 X 33028528 + DMD NNNCTTATATACATCTACCA NGT 2 0.0121 Tier II
9 NC_000007.14 7 133405429 - EXOC4 NNNCTTATATCCATTTACCA NGA 2 0.0046 Tier II
10 NC_000014.9 14 27535714 + MIR3171HG NNNCTTATATCTATCTATCA NGG 2 0.3462 Tier III
11 NC_000015.10 15 98847957 - LOC124903561 NNNGTTATATCCATCTAACA NGG 2 0.2692 Tier III
12 NC_000003.12 3 152169532 + LOC101928166 NNNCTTATATACATATACCA NGG 2 0.1667 Tier III
13 NC_000003.12 3 152169532 + LINC02917 NNNCTTATATACATATACCA NGG 2 0.1667 Tier III
14 NC_000005.10 5 129292896 - LOC102723654 NNNCTCATATCCATCTACCA NGC 2 0.0202 Tier III
15 NC_000012.12 12 59060708 - LRIG3-DT NNNCTGATATCCATCTACCA NGT 2 0.0092 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 81725821 + Map3k9 NNNCTTGTATCCATCTACCA NGG 1 0.7059 Tier I
2 NC_000068.7 2 73493393 + Wipf1 NNNATTACATCCATCTACCA NGG 2 0.8421 Tier II
3 NC_000077.6 11 35186892 + Slit3 NNNCTTATAACCATCTATCA NGG 2 0.551 Tier II
4 NC_000068.7 2 43798881 - Arhgap15 NNNCTATTATCCATCTACCA NGG 2 0.3792 Tier II
5 NC_000067.6 1 78557571 - Mogat1 NNNCTTATAACCATCTACAA NGG 2 0.3673 Tier II
6 NC_000074.6 8 19187287 - Defb14 NNNCTTCTATACATCTACCA NGG 2 0.3281 Tier II
7 NC_000082.6 16 42179665 + Lsamp NNNCTTACATTCATCTACCA NGG 2 0.3077 Tier II
8 NC_000067.6 1 83259604 + Sphkap NNNCTTCTTTCCATCTACCA NGG 2 0.2625 Tier II
9 NC_000083.6 17 44835436 - Supt3 NNNCTTCTCTCCATCTACCA NGG 2 0.25 Tier II
10 NC_000069.6 3 41643349 + Sclt1 NNNCATCTATCCATCTACCA NGG 2 0.2188 Tier II
11 NC_000073.6 7 17882836 + Gm5155 NNNCTTATCTCCTTCTACCA NGG 2 0.1714 Tier II
12 NC_000070.6 4 76764719 - Ptprd NNNCCTATATCCATGTACCA NGG 2 0.05 Tier II
13 NC_000072.6 6 106072292 + Cntn4 NNNCTTATCTCCATCTACCA NGA 2 0.0397 Tier II
14 NC_000070.6 4 15896400 - Calb1 NNNCTTATATCCCTCTCCCA NGG 2 0.0372 Tier II
15 NC_000084.6 18 89521427 + Dok6 NNNCTTATATCCATGTAGCA NGG 2 0.0067 Tier II
16 NC_000069.6 3 52523693 + Gm30173 NNNCTCATATCCATCTACAA NGG 2 0.3896 Tier III
17 NC_000074.6 8 19187287 - Gm39139 NNNCTTCTATACATCTACCA NGG 2 0.3281 Tier III
18 NC_000085.6 19 25838422 - Gm34516 NNNCTTATTTCCACCTACCA NGG 2 0.1714 Tier III
19 NC_000072.6 6 86540863 + 1600020E01Rik NNNTTTATATCCATCTACCA NGT 2 0.0129 Tier III
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Other clones with same target sequence:

(none)