Construct: sgRNA BRDN0001145385
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGCTACGCACAGCTCAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CARD11 (84433)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77678
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
2944262 |
- |
CARD11 |
NNNCTACGCACAGCTCAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
427445 |
+ |
RAB11FIP3 |
NNNCTACGCACAGTACAGTG |
NGG |
2 |
0.4246 |
Tier II |
| 3 |
NC_000001.11 |
1 |
23401107 |
+ |
TCEA3 |
NNNCTGGGCACAGCTCAGTG |
NGG |
2 |
0.3361 |
Tier II |
| 4 |
NC_000004.12 |
4 |
76203652 |
- |
SCARB2 |
NNNCTACACACAGCTCAGAG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000023.11 |
X |
16856246 |
- |
RBBP7 |
NNNCTACGCCCAGCTCATTG |
NGG |
2 |
0.2222 |
Tier II |
| 6 |
NC_000017.11 |
17 |
21001325 |
- |
USP22 |
NNNCTACTCACTGCTCAGTG |
NGG |
2 |
0.2083 |
Tier II |
| 7 |
NC_000012.12 |
12 |
94173882 |
- |
PLXNC1 |
NNNCTACGCGCAGCTCAGAG |
NGG |
2 |
0.1587 |
Tier II |
| 8 |
NC_000001.11 |
1 |
6447578 |
- |
ESPN |
NNNCTAGGCCCAGCTCAGTG |
NGG |
2 |
0.1569 |
Tier II |
| 9 |
NC_000002.12 |
2 |
27870543 |
- |
RBKS |
NNNCTTCGCACAGCTCTGTG |
NGG |
2 |
0.0952 |
Tier II |
| 10 |
NC_000004.12 |
4 |
574244 |
- |
TMEM271 |
NNNCTACGCACAAGTCAGTG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000007.14 |
7 |
2944262 |
- |
CARD11-AS1 |
NNNCTACGCACAGCTCAGTG |
NGG |
0 |
1.0 |
Tier III |
| 12 |
NC_000012.12 |
12 |
94173882 |
- |
LOC124902988 |
NNNCTACGCGCAGCTCAGAG |
NGG |
2 |
0.1587 |
Tier III |
| 13 |
NC_000001.11 |
1 |
16701082 |
+ |
ESPNP |
NNNCTAGGCCCAGCTCAGTG |
NGG |
2 |
0.1569 |
Tier III |
| 14 |
NC_000001.11 |
1 |
16701082 |
+ |
LOC124903858 |
NNNCTAGGCCCAGCTCAGTG |
NGG |
2 |
0.1569 |
Tier III |
| 15 |
NT_187369.1 |
1 |
26913 |
+ |
LOC101060324 |
NNNCTAGGCCCAGCTCAGTG |
NGG |
2 |
0.1569 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
140906675 |
- |
Card11 |
NNNCTACGCCCAGCTCAGTG |
NGG |
1 |
0.3333 |
Tier I |
| 2 |
NC_000075.6 |
9 |
114619244 |
- |
Cnot10 |
NNNCTGCGCCCAGCTCAGTG |
NGG |
2 |
0.2381 |
Tier I |
| 3 |
NC_000072.6 |
6 |
85219005 |
- |
Sfxn5 |
NNNCTACCCTCAGCTCAGTG |
NGG |
2 |
0.543 |
Tier II |
| 4 |
NC_000071.6 |
5 |
48601056 |
+ |
Kcnip4 |
NNNCTCCACACAGCTCAGTG |
NGG |
2 |
0.4545 |
Tier II |
| 5 |
NC_000070.6 |
4 |
103736588 |
- |
Dab1 |
NNNCTGCTCACAGCTCAGTG |
NGG |
2 |
0.4464 |
Tier II |
| 6 |
NC_000073.6 |
7 |
25411466 |
- |
Cxcl17 |
NNNCTATGCACAGCTCAGTC |
NGG |
2 |
0.3482 |
Tier II |
| 7 |
NC_000070.6 |
4 |
100309973 |
+ |
Ror1 |
NNNCTCCTCACAGCTCAGTG |
NGG |
2 |
0.2841 |
Tier II |
| 8 |
NC_000067.6 |
1 |
34235601 |
- |
Dst |
NNNCTACACACAGCTCAGCG |
NGG |
2 |
0.2759 |
Tier II |
| 9 |
NC_000072.6 |
6 |
17086912 |
- |
Tes |
NNNCTACACACAGCTCAGTG |
NAG |
2 |
0.2593 |
Tier II |
| 10 |
NC_000067.6 |
1 |
39469151 |
+ |
Tbc1d8 |
NNNCTACGCACAGCCCATTG |
NGG |
2 |
0.1818 |
Tier II |
| 11 |
NC_000070.6 |
4 |
136950485 |
+ |
Epha8 |
NNNCTACGCACAGCCCAGAG |
NGG |
2 |
0.0779 |
Tier II |
| 12 |
NC_000072.6 |
6 |
104811220 |
+ |
Cntn6 |
NNNCTACGCACAGTGCAGTG |
NGG |
2 |
0.0367 |
Tier II |
| 13 |
NC_000071.6 |
5 |
116144744 |
- |
Ccdc60 |
NNNCTACGGACAGCTCAGTG |
NTG |
2 |
0.0241 |
Tier II |
| 14 |
NC_000073.6 |
7 |
25411466 |
- |
4732471J01Rik |
NNNCTATGCACAGCTCAGTC |
NGG |
2 |
0.3482 |
Tier III |
| 15 |
NC_000072.6 |
6 |
17086912 |
- |
Gm4876 |
NNNCTACACACAGCTCAGTG |
NAG |
2 |
0.2593 |
Tier III |
Other clones with same target sequence:
(none)
