Construct: sgRNA BRDN0001145393
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGAGCGCACCAACCAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR2B (93)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75484
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
38477363 |
+ |
ACVR2B |
NNNGAGCGCACCAACCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
44579585 |
- |
TMED4 |
NNNGAGAGCACCAACCAGAG |
NGT |
2 |
0.0121 |
Tier I |
| 3 |
NC_000007.14 |
7 |
4791952 |
- |
AP5Z1 |
NNNAAGCGCACCACCCAGAG |
NGG |
2 |
0.1929 |
Tier II |
| 4 |
NC_000001.11 |
1 |
20722738 |
- |
SH2D5 |
NNNGAGCGCAGCAGCCAGAG |
NGG |
2 |
0.1167 |
Tier II |
| 5 |
NC_000017.11 |
17 |
78879907 |
+ |
TIMP2 |
NNNGAGCGCACCCGCCAGAG |
NGG |
2 |
0.0982 |
Tier II |
| 6 |
NC_000004.12 |
4 |
80196710 |
+ |
PRDM8 |
NNNGAACGCACCAAGCAGAG |
NGG |
2 |
0.05 |
Tier II |
| 7 |
NC_000004.12 |
4 |
52712474 |
- |
DANCR |
NNNGGGCGCACAAACCAGAG |
NGG |
2 |
0.5143 |
Tier III |
| 8 |
NC_000003.12 |
3 |
13716767 |
+ |
LINC00620 |
NNNGAGTCCACCAACCAGAG |
NGG |
2 |
0.5 |
Tier III |
| 9 |
NC_000022.11 |
22 |
11627727 |
- |
LOC100996699 |
NNNGTGCGCACCAACCAGAG |
NGG |
1 |
0.3636 |
Tier III |
| 10 |
NC_000024.10 |
Y |
10195458 |
- |
LOC101059962 |
NNNGCGCGCACCCACCAGAG |
NGG |
2 |
0.1053 |
Tier III |
| 11 |
NC_000007.14 |
7 |
91378056 |
+ |
LOC101927473 |
NNNGAGCACACCAACCAGAG |
NGA |
2 |
0.0694 |
Tier III |
| 12 |
NC_000007.14 |
7 |
91378056 |
+ |
LINC02932 |
NNNGAGCACACCAACCAGAG |
NGA |
2 |
0.0694 |
Tier III |
| 13 |
NC_000004.12 |
4 |
80196710 |
+ |
PRDM8-AS1 |
NNNGAACGCACCAAGCAGAG |
NGG |
2 |
0.05 |
Tier III |
| 14 |
NC_000021.9 |
21 |
38089912 |
+ |
DSCR4 |
NNNGGGCGCACCAACCAGAG |
NGA |
2 |
0.05 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
119427531 |
+ |
Acvr2b |
NNNGAGCGCACCAACCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
6271757 |
- |
Tmed4 |
NNNGAGAGCACCAACCAGAG |
NGT |
2 |
0.0121 |
Tier I |
| 3 |
NC_000086.7 |
X |
140598688 |
- |
Tsc22d3 |
NNNGAGCGCTCCAACCAGAA |
NGG |
2 |
0.8272 |
Tier II |
| 4 |
NC_000068.7 |
2 |
74857872 |
- |
Mtx2 |
NNNGAGTGCACCAACCAAAG |
NGG |
2 |
0.5625 |
Tier II |
| 5 |
NC_000084.6 |
18 |
56568942 |
- |
Phax |
NNNGAAGGCACCAACCAGAG |
NGG |
2 |
0.4706 |
Tier II |
| 6 |
NC_000084.6 |
18 |
56568942 |
- |
Aldh7a1 |
NNNGAAGGCACCAACCAGAG |
NGG |
2 |
0.4706 |
Tier II |
| 7 |
NC_000085.6 |
19 |
48421325 |
- |
Sorcs3 |
NNNGAGGGCTCCAACCAGAG |
NGG |
2 |
0.4152 |
Tier II |
| 8 |
NC_000075.6 |
9 |
66524699 |
+ |
Usp3 |
NNNGAGCTCACTAACCAGAG |
NGG |
2 |
0.3365 |
Tier II |
| 9 |
NC_000085.6 |
19 |
57813030 |
+ |
Atrnl1 |
NNNTAGCCCACCAACCAGAG |
NGG |
2 |
0.2238 |
Tier II |
| 10 |
NC_000078.6 |
12 |
117246049 |
+ |
Ptprn2 |
NNNGAGCGAACCAATCAGAG |
NGG |
2 |
0.0571 |
Tier II |
| 11 |
NC_000071.6 |
5 |
30126521 |
- |
Hadha |
NNNGAGGGCACCAAGCAGAG |
NGG |
2 |
0.0235 |
Tier II |
| 12 |
NC_000071.6 |
5 |
103010410 |
- |
Mapk10 |
NNNGAGCACACCAACCAGAG |
NGT |
2 |
0.0161 |
Tier II |
| 13 |
NC_000081.6 |
15 |
96319652 |
+ |
Arid2 |
NNNGTGCGCACCAACCAGAG |
NGT |
2 |
0.0059 |
Tier II |
| 14 |
NC_000068.7 |
2 |
180580747 |
+ |
Gm30144 |
NNNGATCGCACAAACCAGAG |
NGG |
2 |
0.4762 |
Tier III |
| 15 |
NC_000079.6 |
13 |
21233403 |
- |
Gm32525 |
NNNGAGAGGACCAACCAGAG |
NGG |
2 |
0.4643 |
Tier III |
| 16 |
NC_000085.6 |
19 |
57343909 |
- |
B230217O12Rik |
NNNGAGGGCACCAACCAGAA |
NGG |
2 |
0.4412 |
Tier III |
| 17 |
NC_000068.7 |
2 |
138615478 |
- |
Gm14061 |
NNNGAGCCCACCAACCAAAG |
NGG |
2 |
0.426 |
Tier III |
| 18 |
NC_000078.6 |
12 |
117246049 |
+ |
Gm5441 |
NNNGAGCGAACCAATCAGAG |
NGG |
2 |
0.0571 |
Tier III |
Other clones with same target sequence:
(none)
