Construct: sgRNA BRDN0001145419

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTAGTTGGTGAGGATAGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A1 (1457)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76673

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 488766 - CSNK2A1 NNNGTTGGTGAGGATAGCCA NGG 0 1.0 Tier I
2 NC_000016.10 16 89198819 + SLC22A31 NNNGTTGGTGAGGATGCCCA NGG 2 0.0452 Tier I
3 NC_000011.10 11 11352384 - CSNK2A3 NNNGTTGGTGAGGATAGCCA NGT 1 0.0161 Tier I
4 NC_000020.11 20 8238192 + PLCB1 NNNGTTGGTGGGAATAGCCA NGG 2 0.6 Tier II
5 NC_000013.11 13 99206489 - UBAC2 NNNGCTGGTGAGCATAGCCA NGG 2 0.4211 Tier II
6 NC_000012.12 12 1416481 - ERC1 NNNGTGGGTGGGGATAGCCA NGG 2 0.3714 Tier II
7 NC_000010.11 10 75603897 + LRMDA NNNGTTGATGAGGACAGCCA NGG 2 0.2727 Tier II
8 NC_000007.14 7 123971025 - SPAM1 NNNGTTGGTGATGATAGTCA NGG 2 0.2473 Tier II
9 NC_000003.12 3 123955347 - CCDC14 NNNGGTGGTGAGTATAGCCA NGG 2 0.192 Tier II
10 NC_000006.12 6 52421864 + EFHC1 NNNGTTGGTGAGTATAGACA NGG 2 0.1615 Tier II
11 NC_000003.12 3 46901994 - PTH1R NNNGTGGGTGAGGACAGCCA NGG 2 0.1558 Tier II
12 NC_000002.12 2 3200850 + EIPR1 NNNCTTGGTGAGGACAGCCA NGG 2 0.1444 Tier II
13 NC_000019.10 19 44867719 + NECTIN2 NNNGCTGGTGAGGATAGGCA NGG 2 0.1333 Tier II
14 NC_000015.10 15 28948910 + APBA2 NNNGTTGGTGAGGATAGGCA NGG 1 0.1333 Tier II
15 NC_000020.11 20 32195343 - PLAGL2 NNNGTTGGTGTGGATAGCAA NGG 2 0.1319 Tier II
16 NC_000003.12 3 131851606 + CPNE4 NNNGTTGGTGATGATAGCGA NGG 2 0.0481 Tier II
17 NC_000011.10 11 11352384 - GALNT18 NNNGTTGGTGAGGATAGCCA NGT 1 0.0161 Tier II
18 NC_000001.11 1 151513894 + CGN NNNGTTGGTGAGGAGAGCCC NGG 2 0.0114 Tier II
19 NC_000012.12 12 7860426 + SLC2A14 NNNGTTGGTCAGGATAGCCA NGC 2 0.0089 Tier II
20 NC_000012.12 12 56082817 + ERBB3 NNNGTGGGTGAGGATTGCCA NGG 2 0.0 Tier II
21 NC_000016.10 16 20855663 - DCUN1D3 NNNGTTGGTGAGGATCGGCA NGG 2 0.0 Tier II
22 NC_000014.9 14 29118385 + LOC107984685 NNNGTTAGTGAGGATAGCCA NAG 2 0.2593 Tier III
23 NC_000007.14 7 141516199 - AGK-DT NNNGTTGGGGAGGACAGCCA NGG 2 0.1688 Tier III
24 NC_000015.10 15 39513911 + LOC105370784 NNNGTTGATGAGGATAGCCA NGA 2 0.0694 Tier III
25 NC_000003.12 3 88282718 + LOC105377202 NNNGTTGGTGAGGATAGGAA NGG 2 0.0571 Tier III
26 NC_000003.12 3 131851606 + LOC105374113 NNNGTTGGTGATGATAGCGA NGG 2 0.0481 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 152274106 + Csnk2a1 NNNGTTGGTGAGGATAGCCA NGG 0 1.0 Tier I
2 NC_000068.7 2 66090950 - Galnt3 NNNGTTGGTGAGAATAACCA NGG 2 0.8615 Tier I
3 NC_000073.6 7 127786036 - Setd1a NNNGGTGGGGAGGATAGCCA NGG 2 0.3962 Tier I
4 NC_000077.6 11 120110972 - Slc38a10 NNNGTTGCTGAGGATAGCCA NGA 2 0.0427 Tier I
5 NC_000076.6 10 64345451 + Ctnna3 NNNGTTGCTGAAGATAGCCA NGG 2 0.5744 Tier II
6 NC_000079.6 13 94263178 - Scamp1 NNNGTTGGTGAAGTTAGCCA NGG 2 0.4978 Tier II
7 NC_000079.6 13 109506819 + Pde4d NNNGTTGGTGAGGAAAGCCG NGG 2 0.4427 Tier II
8 NC_000080.6 14 32677706 + 3425401B19Rik NNNGTTGGTGGGGATAGCCT NGG 2 0.39 Tier II
9 NC_000078.6 12 116428866 + Ncapg2 NNNGTTGGTGAGGATAGACT NGG 2 0.3231 Tier II
10 NC_000077.6 11 69757323 - Polr2a NNNGTGGGTGAGGATAGACA NGG 2 0.3077 Tier II
11 NC_000076.6 10 76004064 + Gm5134 NNNCTTGGTGAGGTTAGCCA NGG 2 0.2824 Tier II
12 NC_000077.6 11 106166239 - Strada NNNCTTGGTCAGGATAGCCA NGG 2 0.2118 Tier II
13 NC_000079.6 13 54626337 + Faf2 NNNCTTGGTCAGGATAGCCA NGG 2 0.2118 Tier II
14 NC_000068.7 2 27478564 - Brd3 NNNGTTGGTGGGGACAGCCA NGG 2 0.1773 Tier II
15 NC_000068.7 2 26059122 + Nacc2 NNNGGTGGTGAGGACAGCCA NGG 2 0.1745 Tier II
16 NC_000075.6 9 45104341 + Jaml NNNGTTGGGGAGGATACCCA NGG 2 0.1457 Tier II
17 NC_000072.6 6 146728352 - Stk38l NNNGTTGGTAAGGATAGGCA NGG 2 0.1244 Tier II
18 NC_000075.6 9 36861400 + Fez1 NNNGTTGGTGATGATAGCCA NAG 2 0.0997 Tier II
19 NC_000068.7 2 15910495 - Malrd1 NNNGTTGGTGATGATAGCCC NGG 2 0.0874 Tier II
20 NC_000073.6 7 142474684 - Lsp1 NNNGTTGGTGATGCTAGCCA NGG 2 0.0824 Tier II
21 NC_000073.6 7 100920621 - Arhgef17 NNNGTTGGTGAGGAAAGGCA NGG 2 0.0772 Tier II
22 NC_000073.6 7 16578637 - Arhgap35 NNNGTTGGTAAGGATAGCCA NGT 2 0.0151 Tier II
23 NC_000068.7 2 31101534 + Fnbp1 NNNGTTGGTGAGGAGAGCCC NGG 2 0.0114 Tier II
24 NC_000067.6 1 156524967 + Csnk2a3 NNNGTTGGTGAGGATAGCCA NGG 0 1.0 Tier III
25 NC_000070.6 4 75325238 + Gm11255 NNNGTTGGTGAGGATAGCCA NGG 0 1.0 Tier III
26 NC_000084.6 18 81448057 + Gm30288 NNNGTTGATGAGGATAGCCT NGG 2 0.6 Tier III
27 NC_000086.7 X 16610992 + Csnk2a1-ps NNNGTTGGTGAGGAAAGCCA NGG 1 0.5789 Tier III
28 NC_000074.6 8 83952164 + Gm10644 NNNGGTGGTGAGGTTAGCCA NGG 2 0.3413 Tier III
29 NC_000078.6 12 113806964 - Igh NNNGTAGGTGAGGACAGCCA NGG 2 0.2364 Tier III
30 NC_000083.6 17 40854628 - Gm20574 NNNGTTGGTGTGGATAGCCC NGG 2 0.0699 Tier III
31 NC_000080.6 14 14198032 + Csnk2a1-ps1 NNNGTTGGTGAGGAGAGCCA NGG 1 0.05 Tier III
32 NC_000074.6 8 24385413 - Gm30633 NNNGTTGGTGAGGATGGGCA NGG 2 0.0256 Tier III
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Other clones with same target sequence:

(none)