Construct: sgRNA BRDN0001145468
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTCAGCATTTCGGACTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRGUK (136332)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78084
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000007.14 |
7 |
134127642 |
- |
LRGUK |
NNNCAGCATTTCGGACTCTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000004.12 |
4 |
13484141 |
- |
RAB28 |
NNNCACCATGTCGGACTCTG |
NGG |
2 |
0.3409 |
Tier I |
3 |
NC_000004.12 |
4 |
5689214 |
- |
EVC2 |
NNNCACCATTTGGGACTCTG |
NGG |
2 |
0.303 |
Tier I |
4 |
NC_000014.9 |
14 |
79195652 |
- |
NRXN3 |
NNNCAGAATTTCAGACTCTG |
NGG |
2 |
0.6923 |
Tier II |
5 |
NC_000008.11 |
8 |
23161305 |
- |
TNFRSF10D |
NNNCAGAATCTCGGACTCTG |
NGG |
2 |
0.4 |
Tier II |
6 |
NC_000014.9 |
14 |
100299637 |
- |
SLC25A29 |
NNNCAGCATTTAGGACTCTG |
NAG |
2 |
0.1852 |
Tier II |
7 |
NC_000020.11 |
20 |
9542221 |
- |
PAK5 |
NNNCAGCTTTTCTGACTCTG |
NGG |
2 |
0.1286 |
Tier II |
8 |
NC_000002.12 |
2 |
177251407 |
- |
NFE2L2 |
NNNCAGCATTTCTTACTCTG |
NGG |
2 |
0.08 |
Tier II |
9 |
NC_000004.12 |
4 |
6403284 |
- |
PPP2R2C |
NNNCAGCATTTCTGTCTCTG |
NGG |
2 |
0.06 |
Tier II |
10 |
NC_000001.11 |
1 |
178369478 |
- |
RASAL2 |
NNNCAGCATTTCGGAGGCTG |
NGG |
2 |
0.0181 |
Tier II |
11 |
NC_000003.12 |
3 |
121231917 |
+ |
STXBP5L |
NNNCAGCATTTTGGACTCTG |
NGT |
2 |
0.0087 |
Tier II |
12 |
NC_000014.9 |
14 |
79195652 |
- |
LOC124903352 |
NNNCAGAATTTCAGACTCTG |
NGG |
2 |
0.6923 |
Tier III |
13 |
NC_000007.14 |
7 |
119494090 |
- |
LOC124901816 |
NNNCAGCATTTTGGAATCTG |
NGG |
2 |
0.5385 |
Tier III |
14 |
NC_000022.11 |
22 |
26715726 |
- |
MIATNB |
NNNCAGCATTTTGGACTCTA |
NGG |
2 |
0.5048 |
Tier III |
15 |
NC_000006.12 |
6 |
118453193 |
+ |
LOC644303 |
NNNCAGCATTTAGGACTTTG |
NGG |
2 |
0.4592 |
Tier III |
16 |
NC_000007.14 |
7 |
9979598 |
+ |
LOC105375146 |
NNNCAGCTTTCCGGACTCTG |
NGG |
2 |
0.2857 |
Tier III |
17 |
NC_000005.10 |
5 |
165240747 |
- |
LINC01938 |
NNNCAGCTTTTCTGACTCTG |
NGG |
2 |
0.1286 |
Tier III |
18 |
NC_000007.14 |
7 |
23026714 |
- |
LOC105375185 |
NNNCAGCATTTCTGACTCTG |
NAG |
2 |
0.0778 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000076.6 |
10 |
68541752 |
- |
Cabcoco1 |
NNNCAGCACTTCTGACTCTG |
NGG |
2 |
0.2769 |
Tier I |
2 |
NC_000080.6 |
14 |
54696907 |
+ |
Gm29776 |
NNNCAGCATTTTGGACTCCG |
NGG |
2 |
0.1485 |
Tier I |
3 |
NC_000084.6 |
18 |
31814178 |
- |
Wdr33 |
NNNCAGCACTTAGGACTCTG |
NGG |
2 |
0.6593 |
Tier II |
4 |
NC_000077.6 |
11 |
103381843 |
+ |
Plekhm1 |
NNNCAGCACTTCGGGCTCTG |
NGG |
2 |
0.6 |
Tier II |
5 |
NC_000067.6 |
1 |
127419249 |
- |
Mgat5 |
NNNTAGCATTACGGACTCTG |
NGG |
2 |
0.6 |
Tier II |
6 |
NC_000068.7 |
2 |
90500634 |
- |
Ptprj |
NNNCAGCATATTGGACTCTG |
NGG |
2 |
0.4615 |
Tier II |
7 |
NC_000078.6 |
12 |
73152393 |
- |
Mnat1 |
NNNCAGCATTTGGGAATCTG |
NGG |
2 |
0.4444 |
Tier II |
8 |
NC_000086.7 |
X |
140415517 |
+ |
Frmpd3 |
NNNCAGCATTTGGGAATCTG |
NGG |
2 |
0.4444 |
Tier II |
9 |
NC_000077.6 |
11 |
44704053 |
- |
Ebf1 |
NNNCAGCATTTGGGACTCTG |
NGG |
1 |
0.4444 |
Tier II |
10 |
NC_000077.6 |
11 |
105264816 |
- |
Tlk2 |
NNNCAGCATTTAGGACACTG |
NGG |
2 |
0.381 |
Tier II |
11 |
NC_000069.6 |
3 |
9606118 |
+ |
Zfp704 |
NNNCAGCATCTCGGACCCTG |
NGG |
2 |
0.3765 |
Tier II |
12 |
NC_000067.6 |
1 |
182626353 |
+ |
Capn8 |
NNNCAGCATTTAGCACTCTG |
NGG |
2 |
0.3061 |
Tier II |
13 |
NC_000075.6 |
9 |
108793543 |
+ |
Ip6k2 |
NNNCAGCATTTCAGATTCTG |
NGG |
2 |
0.284 |
Tier II |
14 |
NC_000068.7 |
2 |
69865731 |
- |
Ssb |
NNNCTGCATTTAGGACTCTG |
NGG |
2 |
0.2597 |
Tier II |
15 |
NC_000081.6 |
15 |
89047380 |
+ |
Mov10l1 |
NNNCAGCATTTGGGACACTG |
NGG |
2 |
0.237 |
Tier II |
16 |
NC_000075.6 |
9 |
121433609 |
+ |
Trak1 |
NNNCAGGATTTGGGACTCTG |
NGG |
2 |
0.2092 |
Tier II |
17 |
NC_000081.6 |
15 |
81156309 |
- |
Mrtfa |
NNNCAGCATTTCGGAGTCTG |
NGG |
1 |
0.1538 |
Tier II |
18 |
NC_000069.6 |
3 |
121167148 |
- |
Rwdd3 |
NNNCAGCATTTGGGACTCAG |
NGG |
2 |
0.127 |
Tier II |
19 |
NC_000081.6 |
15 |
72878151 |
+ |
Trappc9 |
NNNCAGCTTTTCGGCCTCTG |
NGG |
2 |
0.1169 |
Tier II |
20 |
NC_000082.6 |
16 |
41629660 |
+ |
Lsamp |
NNNCAGCATTTCGGAGTTTG |
NGG |
2 |
0.0989 |
Tier II |
21 |
NC_000071.6 |
5 |
65938576 |
+ |
Chrna9 |
NNNCAGCATTTCGGAGACTG |
NGG |
2 |
0.0821 |
Tier II |
22 |
NC_000071.6 |
5 |
63924303 |
- |
Rell1 |
NNNCAGCATTTGGGAGTCTG |
NGG |
2 |
0.0684 |
Tier II |
23 |
NC_000074.6 |
8 |
84368297 |
+ |
Cacna1a |
NNNCAGCATTTGGGAGTCTG |
NGG |
2 |
0.0684 |
Tier II |
24 |
NC_000076.6 |
10 |
59773099 |
+ |
Micu1 |
NNNCAGCATTTGGGAGTCTG |
NGG |
2 |
0.0684 |
Tier II |
25 |
NC_000078.6 |
12 |
83035930 |
+ |
Rgs6 |
NNNCAGCATTTGGGAGTCTG |
NGG |
2 |
0.0684 |
Tier II |
26 |
NC_000082.6 |
16 |
38757321 |
+ |
B4galt4 |
NNNCAGCATTTGGGAGTCTG |
NGG |
2 |
0.0684 |
Tier II |
27 |
NC_000084.6 |
18 |
35493223 |
- |
Sil1 |
NNNCAGCATTTGGGAGTCTG |
NGG |
2 |
0.0684 |
Tier II |
28 |
NC_000084.6 |
18 |
36388051 |
+ |
Cystm1 |
NNNCAGCATTTGGGAGTCTG |
NGG |
2 |
0.0684 |
Tier II |
29 |
NC_000082.6 |
16 |
44329482 |
+ |
Sidt1 |
NNNCAGCATTTGGGACTGTG |
NGG |
2 |
0.0593 |
Tier II |
30 |
NC_000070.6 |
4 |
116015861 |
+ |
Faah |
NNNCAGCATTTCGGACGCAG |
NGG |
2 |
0.0336 |
Tier II |
31 |
NC_000068.7 |
2 |
105005300 |
- |
Eif3m |
NNNCAGCATTTCGGAGGCTG |
NGG |
2 |
0.0181 |
Tier II |
32 |
NC_000076.6 |
10 |
86896308 |
- |
Stab2 |
NNNCAGCATTTCGGAGGCTG |
NGG |
2 |
0.0181 |
Tier II |
33 |
NC_000074.6 |
8 |
125665416 |
+ |
Gm30871 |
NNNCAGCATTTAGGACTCTG |
NGG |
1 |
0.7143 |
Tier III |
34 |
NC_000067.6 |
1 |
127419249 |
- |
Gm51746 |
NNNTAGCATTACGGACTCTG |
NGG |
2 |
0.6 |
Tier III |
35 |
NC_000070.6 |
4 |
48926964 |
- |
Gm12436 |
NNNCAGCATTTTGGACTCTG |
NGG |
1 |
0.5385 |
Tier III |
36 |
NC_000084.6 |
18 |
5535140 |
- |
Gm10125 |
NNNCAGCTTTTCTGACTCTG |
NGG |
2 |
0.1286 |
Tier III |
Other clones with same target sequence:
(none)