Construct: sgRNA BRDN0001145474
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGACCCCTGTCATGACAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- BRD4 (23476)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000019.10 | 19 | 15265485 | - | BRD4 | NNNGACCCCTGTCATGACAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000019.10 | 19 | 15265491 | + | BRD4 | NNNCACCACTGTCATGACAG | NGG | 2 | 0.3441 | Tier I |
3 | NC_000018.10 | 18 | 32674807 | + | KLHL14 | NNNGCCCCCTGTAATGACAG | NGG | 2 | 0.1923 | Tier I |
4 | NC_000019.10 | 19 | 42092464 | + | POU2F2 | NNNGACCCCTGTCATAACAA | NGG | 2 | 0.9375 | Tier II |
5 | NC_000004.12 | 4 | 4553058 | + | LOC124900165 | NNNGACCCCTGTCATGACAT | NGG | 1 | 0.7 | Tier II |
6 | NC_000004.12 | 4 | 146449534 | + | SLC10A7 | NNNGACCCCTGCTATGACAG | NGG | 2 | 0.6632 | Tier II |
7 | NC_000017.11 | 17 | 9488519 | - | STX8 | NNNGACCCTTGTCATGACAT | NGG | 2 | 0.6125 | Tier II |
8 | NC_000004.12 | 4 | 85513934 | + | ARHGAP24 | NNNGACGACTGTCATGACAG | NGG | 2 | 0.3059 | Tier II |
9 | NC_000002.12 | 2 | 23650728 | - | KLHL29 | NNNAACCCCTGTCATGACAG | NAG | 2 | 0.2333 | Tier II |
10 | NC_000003.12 | 3 | 37903839 | - | CTDSPL | NNNGAACCCTGTCCTGACAG | NGG | 2 | 0.199 | Tier II |
11 | NC_000003.12 | 3 | 171264916 | + | TNIK | NNNGACCCCTGCCATGAGAG | NGG | 2 | 0.1263 | Tier II |
12 | NC_000004.12 | 4 | 85513928 | - | ARHGAP24 | NNNGACCCCTGTCATGACAG | NCG | 1 | 0.1071 | Tier II |
13 | NC_000004.12 | 4 | 142298331 | - | INPP4B | NNNGACCCCTTTCATGACAG | NAG | 2 | 0.0997 | Tier II |
14 | NC_000004.12 | 4 | 6552628 | + | PPP2R2C | NNNGACCCCTGTGATGACAT | NGG | 2 | 0.0955 | Tier II |
15 | NC_000011.10 | 11 | 12521692 | - | PARVA | NNNGACCCCTGTGATGACAT | NGG | 2 | 0.0955 | Tier II |
16 | NC_000022.11 | 22 | 38733242 | + | GTPBP1 | NNNGACCCCTGGCATGGCAG | NGG | 2 | 0.0882 | Tier II |
17 | NC_000016.10 | 16 | 81529646 | - | CMIP | NNNGACCCCTGTGATGACAC | NGG | 2 | 0.0584 | Tier II |
18 | NC_000022.11 | 22 | 41572185 | + | CSDC2 | NNNGACCCTTGTCAGGACAG | NGG | 2 | 0.0438 | Tier II |
19 | NC_000001.11 | 1 | 245223454 | + | KIF26B | NNNGACTCCTGTCAGGACAG | NGG | 2 | 0.0406 | Tier II |
20 | NC_000013.11 | 13 | 113318900 | + | LAMP1 | NNNGACCCCTGTCCTGCCAG | NGG | 2 | 0.0378 | Tier II |
21 | NC_000009.12 | 9 | 28098807 | - | LINGO2 | NNNGACCCCTGTCAAGACAG | NTG | 2 | 0.0226 | Tier II |
22 | NC_000019.10 | 19 | 8825941 | - | ZNF558 | NNNGACCCCTGTAATCACAG | NGG | 2 | 0.0 | Tier II |
23 | NC_000011.10 | 11 | 74865053 | + | XRRA1 | NNNGACCCCTGTCACTACAG | NGG | 2 | 0.0 | Tier II |
24 | NC_000008.11 | 8 | 17534402 | + | SLC7A2 | NNNGACCCCTGTCATCACAA | NGG | 2 | 0.0 | Tier II |
25 | NC_000004.12 | 4 | 4553058 | + | STX18-AS1 | NNNGACCCCTGTCATGACAT | NGG | 1 | 0.7 | Tier III |
26 | NC_000011.10 | 11 | 116839362 | - | APOA1-AS | NNNGACCCCTGTGATGACAA | NGG | 2 | 0.1278 | Tier III |
27 | NC_000009.12 | 9 | 84448272 | - | LOC102724036 | NNNGACCCCTGTGATGACAC | NGG | 2 | 0.0584 | Tier III |
28 | NC_000009.12 | 9 | 84448272 | - | LOC124902192 | NNNGACCCCTGTGATGACAC | NGG | 2 | 0.0584 | Tier III |
29 | NC_000001.11 | 1 | 245223454 | + | KIF26B-AS1 | NNNGACTCCTGTCAGGACAG | NGG | 2 | 0.0406 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000077.6 | 11 | 99985181 | - | Krtap16-1 | NNNGACACCTGTCAAGACAG | NGG | 2 | 0.4342 | Tier I |
2 | NC_000067.6 | 1 | 162315810 | + | Dnm3 | NNNGACCCATGACATGACAG | NGG | 2 | 0.6857 | Tier II |
3 | NC_000085.6 | 19 | 40661897 | + | Entpd1 | NNNGGCCTCTGTCATGACAG | NGG | 2 | 0.63 | Tier II |
4 | NC_000069.6 | 3 | 66103779 | - | Veph1 | NNNGACTCCTGTCAAGACAG | NGG | 2 | 0.4704 | Tier II |
5 | NC_000085.6 | 19 | 40661891 | - | Entpd1 | NNNGCCCTCTGTCATGACAG | NGG | 2 | 0.4375 | Tier II |
6 | NC_000070.6 | 4 | 152229461 | + | Acot7 | NNNCACCACTGTCATGACAG | NGG | 2 | 0.3441 | Tier II |
7 | NC_000078.6 | 12 | 83318295 | + | Dpf3 | NNNCACCACTGTCATGACAG | NGG | 2 | 0.3441 | Tier II |
8 | NC_000078.6 | 12 | 83318289 | - | Dpf3 | NNNGCCCACTGTCATGACAG | NGG | 2 | 0.325 | Tier II |
9 | NC_000083.6 | 17 | 74270602 | - | Memo1 | NNNGTCACCTGTCATGACAG | NGG | 2 | 0.2727 | Tier II |
10 | NC_000074.6 | 8 | 24014118 | - | Zmat4 | NNNGACCCCTGTAAAGACAG | NGG | 2 | 0.2227 | Tier II |
11 | NC_000080.6 | 14 | 27919739 | - | Erc2 | NNNGACCCCTGTAATGACTG | NGG | 2 | 0.2071 | Tier II |
12 | NC_000073.6 | 7 | 63722939 | - | Otud7a | NNNGACCACTGTCATGGCAG | NGG | 2 | 0.1147 | Tier II |
13 | NC_000076.6 | 10 | 44451370 | - | Prdm1 | NNNGACACCTGTCATGAGAG | NGG | 2 | 0.1 | Tier II |
14 | NC_000073.6 | 7 | 48975719 | + | Nav2 | NNNGACCCCTGTCCTGACAG | NAG | 2 | 0.0556 | Tier II |
15 | NC_000070.6 | 4 | 142222687 | + | Kazn | NNNGACCCCTGTCATGACTG | NGA | 2 | 0.0374 | Tier II |
16 | NC_000068.7 | 2 | 20551572 | + | Etl4 | NNNGAGCCCTGTCATGACAG | NTG | 2 | 0.0195 | Tier II |
17 | NC_000072.6 | 6 | 111433927 | - | Grm7 | NNNGAACCCTGTCATCACAG | NGG | 2 | 0.0 | Tier II |
18 | NC_000083.6 | 17 | 74270602 | - | Gm52299 | NNNGTCACCTGTCATGACAG | NGG | 2 | 0.2727 | Tier III |
19 | NC_000070.6 | 4 | 48821945 | - | Gm52718 | NNNGACCCCTGTAATGACAG | NGA | 2 | 0.0267 | Tier III |