Construct: sgRNA BRDN0001145474

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAGACCCCTGTCATGACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD4 (23476)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77347

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 15265485 - BRD4 NNNGACCCCTGTCATGACAG NGG 0 1.0 Tier I
2 NC_000019.10 19 15265491 + BRD4 NNNCACCACTGTCATGACAG NGG 2 0.3441 Tier I
3 NC_000018.10 18 32674807 + KLHL14 NNNGCCCCCTGTAATGACAG NGG 2 0.1923 Tier I
4 NC_000019.10 19 42092464 + POU2F2 NNNGACCCCTGTCATAACAA NGG 2 0.9375 Tier II
5 NC_000004.12 4 4553058 + LOC124900165 NNNGACCCCTGTCATGACAT NGG 1 0.7 Tier II
6 NC_000004.12 4 146449534 + SLC10A7 NNNGACCCCTGCTATGACAG NGG 2 0.6632 Tier II
7 NC_000017.11 17 9488519 - STX8 NNNGACCCTTGTCATGACAT NGG 2 0.6125 Tier II
8 NC_000004.12 4 85513934 + ARHGAP24 NNNGACGACTGTCATGACAG NGG 2 0.3059 Tier II
9 NC_000002.12 2 23650728 - KLHL29 NNNAACCCCTGTCATGACAG NAG 2 0.2333 Tier II
10 NC_000003.12 3 37903839 - CTDSPL NNNGAACCCTGTCCTGACAG NGG 2 0.199 Tier II
11 NC_000003.12 3 171264916 + TNIK NNNGACCCCTGCCATGAGAG NGG 2 0.1263 Tier II
12 NC_000004.12 4 85513928 - ARHGAP24 NNNGACCCCTGTCATGACAG NCG 1 0.1071 Tier II
13 NC_000004.12 4 142298331 - INPP4B NNNGACCCCTTTCATGACAG NAG 2 0.0997 Tier II
14 NC_000004.12 4 6552628 + PPP2R2C NNNGACCCCTGTGATGACAT NGG 2 0.0955 Tier II
15 NC_000011.10 11 12521692 - PARVA NNNGACCCCTGTGATGACAT NGG 2 0.0955 Tier II
16 NC_000022.11 22 38733242 + GTPBP1 NNNGACCCCTGGCATGGCAG NGG 2 0.0882 Tier II
17 NC_000016.10 16 81529646 - CMIP NNNGACCCCTGTGATGACAC NGG 2 0.0584 Tier II
18 NC_000022.11 22 41572185 + CSDC2 NNNGACCCTTGTCAGGACAG NGG 2 0.0438 Tier II
19 NC_000001.11 1 245223454 + KIF26B NNNGACTCCTGTCAGGACAG NGG 2 0.0406 Tier II
20 NC_000013.11 13 113318900 + LAMP1 NNNGACCCCTGTCCTGCCAG NGG 2 0.0378 Tier II
21 NC_000009.12 9 28098807 - LINGO2 NNNGACCCCTGTCAAGACAG NTG 2 0.0226 Tier II
22 NC_000019.10 19 8825941 - ZNF558 NNNGACCCCTGTAATCACAG NGG 2 0.0 Tier II
23 NC_000011.10 11 74865053 + XRRA1 NNNGACCCCTGTCACTACAG NGG 2 0.0 Tier II
24 NC_000008.11 8 17534402 + SLC7A2 NNNGACCCCTGTCATCACAA NGG 2 0.0 Tier II
25 NC_000004.12 4 4553058 + STX18-AS1 NNNGACCCCTGTCATGACAT NGG 1 0.7 Tier III
26 NC_000011.10 11 116839362 - APOA1-AS NNNGACCCCTGTGATGACAA NGG 2 0.1278 Tier III
27 NC_000009.12 9 84448272 - LOC102724036 NNNGACCCCTGTGATGACAC NGG 2 0.0584 Tier III
28 NC_000009.12 9 84448272 - LOC124902192 NNNGACCCCTGTGATGACAC NGG 2 0.0584 Tier III
29 NC_000001.11 1 245223454 + KIF26B-AS1 NNNGACTCCTGTCAGGACAG NGG 2 0.0406 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 99985181 - Krtap16-1 NNNGACACCTGTCAAGACAG NGG 2 0.4342 Tier I
2 NC_000067.6 1 162315810 + Dnm3 NNNGACCCATGACATGACAG NGG 2 0.6857 Tier II
3 NC_000085.6 19 40661897 + Entpd1 NNNGGCCTCTGTCATGACAG NGG 2 0.63 Tier II
4 NC_000069.6 3 66103779 - Veph1 NNNGACTCCTGTCAAGACAG NGG 2 0.4704 Tier II
5 NC_000085.6 19 40661891 - Entpd1 NNNGCCCTCTGTCATGACAG NGG 2 0.4375 Tier II
6 NC_000070.6 4 152229461 + Acot7 NNNCACCACTGTCATGACAG NGG 2 0.3441 Tier II
7 NC_000078.6 12 83318295 + Dpf3 NNNCACCACTGTCATGACAG NGG 2 0.3441 Tier II
8 NC_000078.6 12 83318289 - Dpf3 NNNGCCCACTGTCATGACAG NGG 2 0.325 Tier II
9 NC_000083.6 17 74270602 - Memo1 NNNGTCACCTGTCATGACAG NGG 2 0.2727 Tier II
10 NC_000074.6 8 24014118 - Zmat4 NNNGACCCCTGTAAAGACAG NGG 2 0.2227 Tier II
11 NC_000080.6 14 27919739 - Erc2 NNNGACCCCTGTAATGACTG NGG 2 0.2071 Tier II
12 NC_000073.6 7 63722939 - Otud7a NNNGACCACTGTCATGGCAG NGG 2 0.1147 Tier II
13 NC_000076.6 10 44451370 - Prdm1 NNNGACACCTGTCATGAGAG NGG 2 0.1 Tier II
14 NC_000073.6 7 48975719 + Nav2 NNNGACCCCTGTCCTGACAG NAG 2 0.0556 Tier II
15 NC_000070.6 4 142222687 + Kazn NNNGACCCCTGTCATGACTG NGA 2 0.0374 Tier II
16 NC_000068.7 2 20551572 + Etl4 NNNGAGCCCTGTCATGACAG NTG 2 0.0195 Tier II
17 NC_000072.6 6 111433927 - Grm7 NNNGAACCCTGTCATCACAG NGG 2 0.0 Tier II
18 NC_000083.6 17 74270602 - Gm52299 NNNGTCACCTGTCATGACAG NGG 2 0.2727 Tier III
19 NC_000070.6 4 48821945 - Gm52718 NNNGACCCCTGTAATGACAG NGA 2 0.0267 Tier III
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Other clones with same target sequence:

(none)