Construct: sgRNA BRDN0001145476

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TACGAGAGATCCAAGCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MOK (5891)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75937

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 102265876 - MOK NNNGAGAGATCCAAGCACTG NGG 0 1.0 Tier I
2 NC_000007.14 7 141764835 + TAS2R3 NNNCAGAGATCCAACCACTG NGG 2 0.1444 Tier I
3 NC_000014.9 14 59331279 - DAAM1 NNNGAGAGATCCAGGCACAG NGG 2 0.1333 Tier I
4 NC_000002.12 2 162300177 - IFIH1 NNNGAGAGAACCAAGCACTG NGG 1 0.8571 Tier II
5 NC_000007.14 7 148722361 - CUL1 NNNGAGAGGTCCAAGAACTG NGG 2 0.6667 Tier II
6 NC_000008.11 8 62423022 + NKAIN3 NNNGAGAGATCCAAGAACTC NGG 2 0.4286 Tier II
7 NC_000001.11 1 176995065 + ASTN1 NNNGAGAGACCTAAGCACTG NGG 2 0.2872 Tier II
8 NC_000006.12 6 161561699 - PRKN NNNGAGAGATCCAAGAACTG NAG 2 0.2593 Tier II
9 NC_000007.14 7 36601816 + AOAH NNNGAGAGAACCAAGCACAG NGG 2 0.2449 Tier II
10 NC_000006.12 6 5218198 - LYRM4 NNNGAGAGAGCCAGGCACTG NGG 2 0.2333 Tier II
11 NC_000001.11 1 39107814 - MACF1 NNNGAGAGATCCAAGTAATG NGG 2 0.1657 Tier II
12 NC_000008.11 8 140355639 + TRAPPC9 NNNGAGAGATCACAGCACTG NGG 2 0.1504 Tier II
13 NC_000018.10 18 26514280 + KCTD1 NNNGAGAGAGCCAAGCACAG NGG 2 0.1429 Tier II
14 NC_000005.10 5 175487600 - SFXN1 NNNAAGAGATCCAAGGACTG NGG 2 0.1385 Tier II
15 NC_000004.12 4 47731444 + CORIN NNNGAGAGATCCAAGGCCTG NGG 2 0.0271 Tier II
16 NC_000010.11 10 25271113 - GPR158 NNNGAGAGATCCAAGCACAG NGA 2 0.0198 Tier II
17 NC_000020.11 20 34041076 + RALY NNNCAGAGATCCAAGCACTG NGT 2 0.0085 Tier II
18 NC_000020.11 20 21337609 + XRN2 NNNGAGAGATCCAAGTACTG NGC 2 0.0068 Tier II
19 NC_000012.12 12 32747405 + YARS2 NNNGAGAGATCCAATCACTG NTG 2 0.0056 Tier II
20 NC_000012.12 12 10505772 + LOC105369657 NNNGAGAAGTCCAAGCACTG NGG 2 0.6667 Tier III
21 NC_000022.11 22 43400714 + LINC01639 NNNGAGAGAACAAAGCACTG NGG 2 0.6122 Tier III
22 NC_000004.12 4 182974082 + LOC107986327 NNNCAGAGACCCAAGCACTG NGG 2 0.2824 Tier III
23 NC_000006.12 6 5218198 - LYRM4-AS1 NNNGAGAGAGCCAGGCACTG NGG 2 0.2333 Tier III
24 NC_000003.12 3 198153952 + FAM157A NNNGAGAGATGCCAGCACTG NGG 2 0.0526 Tier III
25 NC_000003.12 3 198153952 + LOC124905354 NNNGAGAGATGCCAGCACTG NGG 2 0.0526 Tier III
26 NC_000007.14 7 34778 - FAM157D NNNGAGAGATGCCAGCACTG NGG 2 0.0526 Tier III
27 NC_000009.12 9 138217614 + FAM157B NNNGAGAGATGCCAGCACTG NGG 2 0.0526 Tier III
28 NC_000016.10 16 90102985 + FAM157C NNNGAGAGATGCCAGCACTG NGG 2 0.0526 Tier III
29 NC_000016.10 16 51312512 + LOC107984886 NNNGAGAGATCCAAGCACTG NAC 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 110828094 - Mok NNNGAGAGATCCAAGCACTG NGG 0 1.0 Tier I
2 NC_000080.6 14 68124415 - Nefm NNNCAGAGATCCAGGCACTG NGG 2 0.2471 Tier I
3 NC_000083.6 17 69253638 + Epb41l3 NNNGAGAATTCCAAGCACTG NGG 2 0.6 Tier II
4 NC_000073.6 7 122046604 - Ears2 NNNGAGAGAGCCAAGCACTA NGG 2 0.4688 Tier II
5 NC_000068.7 2 136364532 - Pak7 NNNGAGACATCAAAGCACTG NGG 2 0.4396 Tier II
6 NC_000071.6 5 111203367 - Ttc28 NNNGAGAGAGACAAGCACTG NGG 2 0.375 Tier II
7 NC_000073.6 7 6790196 + Usp29 NNNGAGAGATCCATGCACTT NGG 2 0.3733 Tier II
8 NC_000068.7 2 96586485 - Lrrc4c NNNAAGAGATCCAAGCACTG NAG 2 0.2333 Tier II
9 NC_000075.6 9 40405934 + Gramd1b NNNGAGAGATCCAGGCACTC NGG 2 0.2 Tier II
10 NC_000071.6 5 148057080 + Mtus2 NNNGAGAGATCCAAGGACTG NGG 1 0.1538 Tier II
11 NC_000077.6 11 106125603 - Map3k3 NNNGGGAGATCCCAGCACTG NGG 2 0.1516 Tier II
12 NC_000082.6 16 44322493 + Sidt1 NNNGAGAGATCCCAGCACTT NGG 2 0.1474 Tier II
13 NC_000070.6 4 46776185 - Gabbr2 NNNGAGAGATCCAAACAGTG NGG 2 0.1255 Tier II
14 NC_000074.6 8 121742346 + Jph3 NNNCAGAGATCCCAGCACTG NGG 2 0.1115 Tier II
15 NC_000071.6 5 77059963 + Thegl NNNGAGTGATCCAAGCACGG NGG 2 0.1094 Tier II
16 NC_000070.6 4 110350143 - Elavl4 NNNGAGAGATCCCAGTACTG NGG 2 0.0648 Tier II
17 NC_000080.6 14 56517263 + Rnf17 NNNTAGAGATCCAAGCTCTG NGG 2 0.0485 Tier II
18 NC_000081.6 15 11347124 + Adamts12 NNNGAGAGATGCAAGCCCTG NGG 2 0.0441 Tier II
19 NC_000080.6 14 68124415 - A230070E04Rik NNNCAGAGATCCAGGCACTG NGG 2 0.2471 Tier III
20 NC_000068.7 2 145705850 + BC039771 NNNGAGAGATCCAAGCATGG NGG 2 0.1607 Tier III
21 NC_000067.6 1 80456999 - 1700016L21Rik NNNGAGAGCTCCCAGCACTG NGG 2 0.1203 Tier III
22 NC_000067.6 1 80456999 - Gm38673 NNNGAGAGCTCCCAGCACTG NGG 2 0.1203 Tier III
23 NC_000075.6 9 61590935 + Gm39350 NNNCAGAGATCCCAGCACTG NGG 2 0.1115 Tier III
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Other clones with same target sequence:

(none)