Construct: sgRNA BRDN0001145489

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCACTGGATGAAAATATGAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PBK (55872)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75573

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 27820570 - PBK NNNCTGGATGAAAATATGAC NGG 0 1.0 Tier I
2 NC_000001.11 1 45824487 - MAST2 NNNGTGGATGAAAATATGTC NGG 2 0.2692 Tier I
3 NC_000008.11 8 144205831 - MROH1 NNNCTGGAAGAAAATATGAC NGG 1 0.9286 Tier II
4 NC_000013.11 13 19960765 - ZMYM2 NNNCTGAAGGAAAATATGAC NGG 2 0.619 Tier II
5 NC_000002.12 2 47045223 + TTC7A NNNTTGGGTGAAAATATGAC NGG 2 0.5867 Tier II
6 NC_000008.11 8 129891025 - CYRIB NNNCTGGAAGAAATTATGAC NGG 2 0.4952 Tier II
7 NC_000011.10 11 106686214 - GUCY1A2 NNNCTGCATGAAAATATTAC NGG 2 0.4583 Tier II
8 NC_000012.12 12 42120230 - GXYLT1 NNNATGGATGAAATTATGAC NGG 2 0.4491 Tier II
9 NC_000010.11 10 127437872 + DOCK1 NNNTAGGATGAAAATATGAC NGG 2 0.4 Tier II
10 NC_000008.11 8 133084657 + SLA NNNCTGTAGGAAAATATGAC NGG 2 0.3537 Tier II
11 NC_000008.11 8 133084657 + TG NNNCTGTAGGAAAATATGAC NGG 2 0.3537 Tier II
12 NC_000016.10 16 78975418 + WWOX NNNCTGGATGAAAATAACAC NGG 2 0.254 Tier II
13 NC_000020.11 20 2394363 + TGM6 NNNCTGGATGAACAAATGAC NGG 2 0.1219 Tier II
14 NC_000021.9 21 26567259 + CYYR1 NNNCTGGATGAAAAGATGAT NGG 2 0.015 Tier II
15 NC_000010.11 10 88286618 + RNLS NNNCTGGATGACAATATGAC NGT 2 0.0042 Tier II
16 NC_000002.12 2 26109619 - RAB10 NNNCTGGATGAAAATCTGAA NGG 2 0.0 Tier II
17 NC_000008.11 8 37508345 - LINC01605 NNNCTGGATTAAAATATGAC NGG 1 0.8125 Tier III
18 NC_000014.9 14 97957751 - LINC01550 NNNATGGATGAAAATATGAA NGG 2 0.4211 Tier III
19 NC_000010.11 10 78448372 + LOC107984245 NNNCTGGATGAAAAAATGAA NGG 2 0.2895 Tier III
20 NC_000004.12 4 94140745 + SMARCAD1-DT NNNCTGGATGGAAACATGAC NGG 2 0.1773 Tier III
21 NC_000002.12 2 176833884 - LOC105373757 NNNCTGGAGGAAAATATGAC NAG 2 0.1605 Tier III
22 NC_000005.10 5 72374221 + RPL10AP8 NNNCTGAATGAAAATATGAC NTG 2 0.039 Tier III
23 NC_000003.12 3 6643114 - GRM7-AS3 NNNCTGGATGAAAATATGAC NTG 1 0.039 Tier III
24 NC_000003.12 3 6643114 - LOC105376944 NNNCTGGATGAAAATATGAC NTG 1 0.039 Tier III
25 NC_000021.9 21 26567259 + CYYR1-AS1 NNNCTGGATGAAAAGATGAT NGG 2 0.015 Tier III
26 NC_000010.11 10 88286618 + LOC101929727 NNNCTGGATGACAATATGAC NGT 2 0.0042 Tier III
27 NC_000018.10 18 41491286 - KC6 NNNCTGGATGAAAACTTGAC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 65815297 + Pbk NNNTTGGATGAAAATATGAC NGG 1 0.8 Tier I
2 NC_000076.6 10 41581402 + Ccdc162 NNNCTGGCTGAAAATATGAC NGA 2 0.0298 Tier I
3 NC_000069.6 3 65206499 + Kcnab1 NNNCTTGATAAAAATATGAC NGG 2 0.6222 Tier II
4 NC_000074.6 8 63313265 + Spock3 NNNTTGGGTGAAAATATGAC NGG 2 0.5867 Tier II
5 NC_000086.7 X 165199765 - Glra2 NNNATGGATGAAAATATTAC NGG 2 0.5614 Tier II
6 NC_000083.6 17 24904066 + Mapk8ip3 NNNCTGGGTGAAAAAATGAC NGG 2 0.4246 Tier II
7 NC_000086.7 X 42823698 + Tenm1 NNNCTGGATGAAGATATGAA NGG 2 0.3261 Tier II
8 NC_000079.6 13 53147592 - Ror2 NNNCTGGAAGAAAATATGAT NGG 2 0.2786 Tier II
9 NC_000077.6 11 81421070 - Asic2 NNNATGGATGAATATATGAC NGG 2 0.2526 Tier II
10 NC_000082.6 16 72432213 + Robo1 NNNCTGGATCAAAGTATGAC NGG 2 0.1867 Tier II
11 NC_000078.6 12 70869470 + Frmd6 NNNCTGTATGAAAATATGAT NGG 2 0.1714 Tier II
12 NC_000080.6 14 60671657 + Spata13 NNNCTGGATGGAAATATGCC NGG 2 0.1345 Tier II
13 NC_000077.6 11 109958574 - Abca8b NNNCTGGCTGAAAATATGAT NGG 2 0.1286 Tier II
14 NC_000073.6 7 27962073 - Zfp780b NNNGTGGATGAAAATGTGAC NGG 2 0.0962 Tier II
15 NC_000084.6 18 63080805 + Piezo2 NNNCTGGATGAAACTATGCC NGG 2 0.0443 Tier II
16 NC_000077.6 11 110495462 - Map2k6 NNNCTGGATGAATATATGAC NGA 2 0.0208 Tier II
17 NC_000072.6 6 104702787 - Cntn6 NNNCTGAATGAAAATATGAC NGT 2 0.0161 Tier II
18 NC_000086.7 X 75410290 - Cmc4 NNNCTGGATTAAAATATGAC NGT 2 0.0131 Tier II
19 NC_000080.6 14 25483229 - Zmiz1 NNNCTGGATGAAAAGATGCC NGG 2 0.0103 Tier II
20 NC_000083.6 17 62859493 + Efna5 NNNCTGGATGAAAATATGAC NAT 2 0.0 Tier II
21 NC_000067.6 1 103297482 + Gm18728 NNNCTGGATGAAAATATGAC NGG 0 1.0 Tier III
22 NC_000069.6 3 39128942 - Gm43308 NNNCTGGATAAGAATATGAC NGG 2 0.6741 Tier III
23 NC_000069.6 3 65206499 + A330015K06Rik NNNCTTGATAAAAATATGAC NGG 2 0.6222 Tier III
24 NC_000075.6 9 69827165 + B230323A14Rik NNNCTGGAAGAAAATATGCC NGG 2 0.1921 Tier III
25 NC_000073.6 7 71095800 - Gm36011 NNNCTGGATGAAAATAGAAC NGG 2 0.0814 Tier III
26 NC_000073.6 7 71095800 - Gm39033 NNNCTGGATGAAAATAGAAC NGG 2 0.0814 Tier III
27 NC_000074.6 8 15534014 + Gm46033 NNNCTGGATGAATATATGAC NGA 2 0.0208 Tier III
28 NC_000070.6 4 39895572 - Gm12387 NNNTTGGATGAAAATATGAC NGT 2 0.0129 Tier III
29 NC_000086.7 X 17111772 + Gm4203 NNNCTGGATGAAAATATGAC NTT 2 0.0 Tier III
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Other clones with same target sequence:

(none)