Construct: sgRNA BRDN0001145494

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGGTCTTAGGCAAACCCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGFR1 (2260)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76067

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 38417974 - FGFR1 NNNGTCTTAGGCAAACCCCT NGG 0 1.0 Tier I
2 NC_000023.11 X 123189016 - GRIA3 NNNGTCTTAAGCAAACCTCT NGG 2 0.6 Tier II
3 NC_000007.14 7 127066973 - GRM8 NNNGTTTTAGGCAAACCACT NGG 2 0.5 Tier II
4 NC_000017.11 17 15336830 - TEKT3 NNNGTCTTAGGCATACCCAT NGG 2 0.2286 Tier II
5 NC_000005.10 5 107669274 + EFNA5 NNNCTCTTAGGCTAACCCCT NGG 2 0.1588 Tier II
6 NC_000020.11 20 43116382 - PTPRT NNNGTCTAAGGCAAAGCCCT NGG 2 0.1231 Tier II
7 NC_000007.14 7 71783974 - CALN1 NNNGTCTTAGGCAAACCCAG NGG 2 0.0756 Tier II
8 NC_000004.12 4 165427192 + CPE NNNGTCTTAGGCAAGCCCCT NGT 2 0.0105 Tier II
9 NC_000009.12 9 118009266 + LOC105376246 NNNGTATTAGGCAAACTCCT NGG 2 0.4333 Tier III
10 NC_000021.9 21 39529416 + JCADP1 NNNGTCTCAGGCTAACCCCT NGG 2 0.3 Tier III
11 NC_000023.11 X 22664016 - PTCHD1-AS NNNGGCTTAGGCCAACCCCT NGG 2 0.1347 Tier III
12 NC_000003.12 3 6763378 - GRM7-AS3 NNNGTCTTAGGCAACCTCCT NGG 2 0.1273 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 25569657 + Fgfr1 NNNGTCTTAGGCAAACCACT NGG 1 0.5385 Tier I
2 NC_000080.6 14 77714457 + Enox1 NNNGTCTTAGGCAAACCCCA NGG 1 0.5625 Tier II
3 NC_000070.6 4 14605175 + Slc26a7 NNNGTATTTGGCAAACCCCT NGG 2 0.5571 Tier II
4 NC_000078.6 12 105218468 - Tcl1 NNNGTCTTAGCCAAACCCCT NGG 1 0.4286 Tier II
5 NC_000069.6 3 152526461 + Ak5 NNNGTCATAGGCAAACTCCT NGG 2 0.4083 Tier II
6 NC_000069.6 3 26770675 - Spata16 NNNGTCTTAGGAAAACACCT NGG 2 0.3333 Tier II
7 NC_000068.7 2 4508084 + Frmd4a NNNGTCTTAGGCAAGCCCTT NGG 2 0.3 Tier II
8 NC_000073.6 7 35510168 - Tdrd12 NNNTTCTTAGGTAAACCCCT NGG 2 0.1958 Tier II
9 NC_000075.6 9 71518651 + Myzap NNNGTCTTAGGCAAACCCCG NGG 1 0.1765 Tier II
10 NC_000071.6 5 149682747 + B3glct NNNGTCTTAGGCAACCCCCA NGG 2 0.1534 Tier II
11 NC_000075.6 9 89604078 - Minar1 NNNGTCTTAGGCAACCCCCA NGG 2 0.1534 Tier II
12 NC_000073.6 7 87693585 + Grm5 NNNGTCTTAGGCAACTCCCT NGG 2 0.0839 Tier II
13 NC_000071.6 5 128864906 - Rimbp2 NNNGTCTTAGGCAAACCCTG NGG 2 0.0814 Tier II
14 NC_000072.6 6 94215677 + Magi1 NNNTTCTTAGGCACACCCCT NGG 2 0.0779 Tier II
15 NC_000068.7 2 7068368 - Celf2 NNNGTCTTAGGCAAAGCCCT NAG 2 0.0399 Tier II
16 NC_000068.7 2 80423062 + Frzb NNNGTCTTAAGCAAACCCCT NTG 2 0.0364 Tier II
17 NC_000074.6 8 120618541 - 1190005I06Rik NNNGTCTTAGGCGAACCCCT NTG 2 0.0254 Tier II
18 NC_000074.6 8 120618541 - Gm27021 NNNGTCTTAGGCGAACCCCT NTG 2 0.0254 Tier II
19 NC_000075.6 9 62365756 + Anp32a NNNGTCTTAGGCAGACCCCT NTG 2 0.0182 Tier II
20 NC_000076.6 10 14532892 - Adgrg6 NNNGTCTTAGGCAAACCCCG NGA 2 0.0123 Tier II
21 NC_000067.6 1 170435177 - Nos1ap NNNGTCTTAGGCAACCCCCT NTG 2 0.0106 Tier II
22 NC_000073.6 7 27993034 + Zfp850 NNNGTCTTAGGCAACCCCCT NTG 2 0.0106 Tier II
23 NC_000078.6 12 70871762 - Frmd6 NNNGTCTTAGGCAACCCCCT NTG 2 0.0106 Tier II
24 NC_000068.7 2 4508084 + Gm39751 NNNGTCTTAGGCAAGCCCTT NGG 2 0.3 Tier III
25 NC_000072.6 6 94215677 + 4930511A08Rik NNNTTCTTAGGCACACCCCT NGG 2 0.0779 Tier III
26 NC_000072.6 6 94215677 + Gm52867 NNNTTCTTAGGCACACCCCT NGG 2 0.0779 Tier III
27 NC_000077.6 11 54215793 + Gm12223 NNNGTCTTTGGCAAACCCCT NGT 2 0.0097 Tier III
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Other clones with same target sequence:

(none)