Construct: sgRNA BRDN0001145499

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCACTTCAAACAATGCTGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NRBP1 (29959)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77018

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 27439837 - NRBP1 NNNCTTCAAACAATGCTGGG NGG 0 1.0 Tier I
2 NC_000004.12 4 48255171 + TEC NNNCTTCAAACAATGCTGAG NGT 2 0.0115 Tier I
3 NC_000016.10 16 77884705 + VAT1L NNNCTTCAAACAAGGCCGGG NGG 2 0.0 Tier I
4 NC_000001.11 1 24139781 - IL22RA1 NNNCCTCAAACAATGCTGGT NGG 2 0.7 Tier II
5 NC_000012.12 12 14411241 - ATF7IP NNNCTTCAAACAATGCTGGT NGG 1 0.7 Tier II
6 NC_000010.11 10 51382599 + PRKG1 NNNCCTCAAACAATGCTGTG NGG 2 0.6667 Tier II
7 NC_000016.10 16 28023566 - GSG1L NNNCTTCACACAATGCTGGT NGG 2 0.4 Tier II
8 NC_000003.12 3 89426805 - EPHA3 NNNCTTCATACAGTGCTGGG NGG 2 0.3913 Tier II
9 NC_000017.11 17 37134669 + ACACA NNNCTTTAAACAATGCTCGG NGG 2 0.3869 Tier II
10 NC_000005.10 5 137666054 - KLHL3 NNNGTTCAAACAATGCTGTG NGG 2 0.3333 Tier II
11 NC_000009.12 9 70758585 + TRPM3 NNNCTTCAAACTATACTGGG NGG 2 0.3137 Tier II
12 NC_000005.10 5 169844581 - DOCK2 NNNCCTCAAACATTGCTGGG NGG 2 0.3 Tier II
13 NC_000017.11 17 30954603 + ADAP2 NNNCCTCAAACATTGCTGGG NGG 2 0.3 Tier II
14 NC_000003.12 3 51621306 + RAD54L2 NNNATTCAAAGAATGCTGGG NGG 2 0.2105 Tier II
15 NC_000012.12 12 32686748 - DNM1L NNNCTTCAAACATTGCTGGT NGG 2 0.21 Tier II
16 NC_000018.10 18 36343066 + FHOD3 NNNCTTCAAACATTGCTGGT NGG 2 0.21 Tier II
17 NC_000009.12 9 35688353 + TPM2 NNNCTTAAAACAATCCTGGG NGG 2 0.2045 Tier II
18 NC_000011.10 11 19699309 - NAV2 NNNCTTCAAACAATGCTTGG NAG 2 0.1728 Tier II
19 NC_000019.10 19 19191894 + BORCS8-MEF2B NNNCTTGAAACAATCCTGGG NGG 2 0.1283 Tier II
20 NC_000019.10 19 19191894 + BORCS8 NNNCTTGAAACAATCCTGGG NGG 2 0.1283 Tier II
21 NC_000010.11 10 95760329 - ENTPD1 NNNCTTCAAGCACTGCTGGG NGG 2 0.117 Tier II
22 NC_000013.11 13 112909364 - MCF2L NNNCTTCAACCCATGCTGGG NGG 2 0.0877 Tier II
23 NC_000001.11 1 41716632 + HIVEP3 NNNCTTCAAACAAAGCTGGG NGA 2 0.043 Tier II
24 NC_000009.12 9 131660596 + RAPGEF1 NNNCTTCAAACAATGCTGCG NTG 2 0.0175 Tier II
25 NC_000005.10 5 176816986 - UNC5A NNNCTTCAAACAGGGCTGGG NGG 2 0.0 Tier II
26 NC_000006.12 6 8071668 + EEF1E1-BLOC1S5 NNNTTTCAAACAATGCTGAG NGG 2 0.5714 Tier III
27 NC_000017.11 17 14878502 + LINC02096 NNNCTTCATACAATGCTGGT NGG 2 0.42 Tier III
28 NC_000017.11 17 44970914 - LOC112268183 NNNCTTTAAACAATGTTGGG NGG 2 0.25 Tier III
29 NC_000004.12 4 165505943 - LOC105377520 NNNCTTCTAACAATGCAGGG NGG 2 0.2286 Tier III
30 NC_000001.11 1 60706612 - LOC101926964 NNNCTCCAAACACTGCTGGG NGG 2 0.1914 Tier III
31 NC_000005.10 5 101824341 - LOC105379102 NNNCTTGAAACAATCCTGGG NGG 2 0.1283 Tier III
32 NC_000010.11 10 95760329 - ENTPD1-AS1 NNNCTTCAAGCACTGCTGGG NGG 2 0.117 Tier III
33 NC_000006.12 6 53457764 - GCLC-AS1 NNNCTTCAAAGTATGCTGGG NGG 2 0.0833 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 31249620 - Nrbp1 NNNCTTCAAACAGTGCTGGG NGG 1 0.6522 Tier I
2 NC_000073.6 7 17757618 - Ceacam5 NNNCCTCCAACAATGCTGGG NGG 2 0.4286 Tier I
3 NC_000073.6 7 17908867 - Gm5155 NNNCCTCCAACAATGCTGGG NGG 2 0.4286 Tier I
4 NC_000073.6 7 111739020 - Galnt18 NNNCTCCAAACGATGCTGGG NGG 2 0.6566 Tier II
5 NC_000083.6 17 32818393 + Zfp799 NNNCTTTAAAAAATGCTGGG NGG 2 0.6094 Tier II
6 NC_000069.6 3 82061930 + Gucy1b1 NNNATTCAAACAGTGCTGGG NGG 2 0.5492 Tier II
7 NC_000068.7 2 4035157 - Frmd4a NNNCTTCAAACAATGCTTGT NGG 2 0.4667 Tier II
8 NC_000077.6 11 100564066 - Ttc25 NNNCTTAAAACAAAGCTGGG NGG 2 0.4643 Tier II
9 NC_000073.6 7 49141294 + Nav2 NNNCTTCATACAATGCTGGT NGG 2 0.42 Tier II
10 NC_000074.6 8 83205750 - Tbc1d9 NNNCTTCACACAAAGCTGGG NGG 2 0.3537 Tier II
11 NC_000077.6 11 33967573 + Kcnmb1 NNNATTCAAACAATGCTGGG NAG 2 0.2183 Tier II
12 NC_000077.6 11 33967573 + Kcnip1 NNNATTCAAACAATGCTGGG NAG 2 0.2183 Tier II
13 NC_000068.7 2 153010512 + Pdrg1 NNNATTCAAAGAATGCTGGG NGG 2 0.2105 Tier II
14 NC_000068.7 2 27096482 + Adamtsl2 NNNCTTCAAACAATCCCGGG NGG 2 0.1925 Tier II
15 NC_000077.6 11 11506429 + Spata48 NNNCTTCAAAGAATGCTTGG NGG 2 0.1667 Tier II
16 NC_000070.6 4 84528471 - Bnc2 NNNCTTCAAACACTGCTGAG NGG 2 0.1504 Tier II
17 NC_000082.6 16 18791414 + Cdc45 NNNCTTCAAACACTGCTGGT NGG 2 0.1474 Tier II
18 NC_000077.6 11 16855332 + Egfr NNNCTTCAAACAATTCTGGG NGG 1 0.1429 Tier II
19 NC_000075.6 9 77222415 - Mlip NNNGTTCAAACAATGCTGGG NAG 2 0.1296 Tier II
20 NC_000074.6 8 11633033 + Ankrd10 NNNCTTCAAACAAAGGTGGG NGG 2 0.0952 Tier II
21 NC_000075.6 9 120162534 + Mobp NNNTTTCAAACAATGCTGGG NGA 2 0.0556 Tier II
22 NC_000069.6 3 105927639 - I830077J02Rik NNNCTTCACACAATGCTGGG NTG 2 0.0223 Tier II
23 NC_000074.6 8 11226676 + Col4a1 NNNCTTCAAACAATGATGGG NGC 2 0.0222 Tier II
24 NC_000075.6 9 106024826 + Col6a4 NNNCTTCAAACAAGGCTGGG NGC 2 0.0 Tier II
25 NC_000073.6 7 49141294 + 9130015G15Rik NNNCTTCATACAATGCTGGT NGG 2 0.42 Tier III
26 NC_000073.6 7 57403858 - Gm9962 NNNCTTCAAACACTGCAGGG NGG 2 0.1123 Tier III
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Other clones with same target sequence:

(none)