Construct: sgRNA BRDN0001145514
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGCAACGAGAATTGGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK3 (6788)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75977
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
98579684 |
- |
STK3 |
NNNGCAACGAGAATTGGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
246380922 |
+ |
SMYD3 |
NNNGCAAAGAGAATTGGAAG |
NGG |
1 |
0.65 |
Tier II |
| 3 |
NC_000007.14 |
7 |
101507231 |
- |
COL26A1 |
NNNGCAAGGAGAATTAGAAG |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000009.12 |
9 |
70750739 |
+ |
TRPM3 |
NNNGAAACGAGAATTGGAAG |
NGG |
1 |
0.5714 |
Tier II |
| 5 |
NC_000008.11 |
8 |
35659056 |
+ |
UNC5D |
NNNGCAATGAGAATAGGAAG |
NGG |
2 |
0.5066 |
Tier II |
| 6 |
NC_000009.12 |
9 |
69721313 |
+ |
PTAR1 |
NNNACAACTAGAATTGGAAG |
NGG |
2 |
0.48 |
Tier II |
| 7 |
NC_000013.11 |
13 |
74141091 |
+ |
KLF12 |
NNNGAAACGAGGATTGGAAG |
NGG |
2 |
0.4127 |
Tier II |
| 8 |
NC_000021.9 |
21 |
42001235 |
- |
ZBTB21 |
NNNGATACGAGAATTGGAAG |
NGG |
2 |
0.4082 |
Tier II |
| 9 |
NC_000003.12 |
3 |
171268807 |
+ |
TNIK |
NNNGGAAAGAGAATTGGAAG |
NGG |
2 |
0.39 |
Tier II |
| 10 |
NC_000003.12 |
3 |
41602395 |
+ |
ULK4 |
NNNGGAAGGAGAATTGGAAG |
NGG |
2 |
0.3857 |
Tier II |
| 11 |
NC_000001.11 |
1 |
202908136 |
+ |
KLHL12 |
NNNGCAAAGAGAATAGGAAG |
NGG |
2 |
0.3763 |
Tier II |
| 12 |
NC_000017.11 |
17 |
58348784 |
- |
SUPT4H1 |
NNNTCAACCAGAATTGGAAG |
NGG |
2 |
0.1958 |
Tier II |
| 13 |
NC_000012.12 |
12 |
107345399 |
+ |
ABTB3 |
NNNGCAAAGAGAATTGGAAG |
NAG |
2 |
0.1685 |
Tier II |
| 14 |
NC_000005.10 |
5 |
135379474 |
+ |
MACROH2A1 |
NNNGCAACCAGAACTGGAAG |
NGG |
2 |
0.1538 |
Tier II |
| 15 |
NC_000016.10 |
16 |
12993854 |
+ |
SHISA9 |
NNNGCAAGGAGAATGGGAAG |
NGG |
2 |
0.0321 |
Tier II |
| 16 |
NC_000001.11 |
1 |
171034983 |
+ |
MROH9 |
NNNGCAACGACAATTGGAAG |
NGC |
2 |
0.0095 |
Tier II |
| 17 |
NC_000001.11 |
1 |
157929928 |
- |
LOC105371458 |
NNNGTAAGGAGAATTGGAAG |
NGG |
2 |
0.4091 |
Tier III |
| 18 |
NC_000008.11 |
8 |
72123865 |
- |
LOC124901962 |
NNNGCAACGGTAATTGGAAG |
NGG |
2 |
0.2137 |
Tier III |
| 19 |
NC_000017.11 |
17 |
58348784 |
- |
TSPOAP1-AS1 |
NNNTCAACCAGAATTGGAAG |
NGG |
2 |
0.1958 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
102044925 |
+ |
Rnf121 |
NNNGGAAGGAGAATTGGAAG |
NGG |
2 |
0.3857 |
Tier II |
| 2 |
NC_000072.6 |
6 |
119166878 |
- |
Cacna1c |
NNNGCATTGAGAATTGGAAG |
NGG |
2 |
0.3828 |
Tier II |
| 3 |
NC_000073.6 |
7 |
56756341 |
+ |
Gabrg3 |
NNNGAAACCAGAATTGGAAG |
NGG |
2 |
0.3077 |
Tier II |
| 4 |
NC_000075.6 |
9 |
7865505 |
+ |
Birc3 |
NNNGCAACGAGGATTGGGAG |
NGG |
2 |
0.2889 |
Tier II |
| 5 |
NC_000072.6 |
6 |
83673054 |
- |
Cd207 |
NNNGCAACCAGAATTGGAAG |
NAG |
2 |
0.1396 |
Tier II |
| 6 |
NC_000082.6 |
16 |
50044837 |
+ |
Gm4827 |
NNNCCAACAAGAATTGGAAG |
NGG |
2 |
0.3403 |
Tier III |
| 7 |
NC_000069.6 |
3 |
88208022 |
+ |
Gm3764 |
NNNGAAACGAGAATTGGGAG |
NGG |
2 |
0.2286 |
Tier III |
Other clones with same target sequence:
(none)
