Construct: sgRNA BRDN0001145539

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCTAAAGAATGTGCAGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK7 (140609)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77739

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 198279047 - NEK7 NNNTAAAGAATGTGCAGCTG NGG 0 1.0 Tier I
2 NC_000006.12 6 42194720 + GUCA1B NNNTAAAGAGTGTGCCGCTG NGG 2 0.0 Tier I
3 NC_000001.11 1 173758027 + KLHL20 NNNTATAGATTGTGCAGCTG NGG 2 0.6303 Tier II
4 NC_000007.14 7 138085745 - AKR1D1 NNNTAAAGAAAGTACAGCTG NGG 2 0.5625 Tier II
5 NC_000018.10 18 79208335 + ATP9B NNNTAAAGAATATGCAGATG NGG 2 0.5026 Tier II
6 NC_000010.11 10 75343717 - ZNF503 NNNTAAAGAATCTGCAGCTA NGG 2 0.4963 Tier II
7 NC_000005.10 5 119510011 - HSD17B4 NNNTAAACAATGTGCAGCTT NGG 2 0.4308 Tier II
8 NC_000006.12 6 17505332 - CAP2 NNNTAAATTATGTGCAGCTG NGG 2 0.375 Tier II
9 NC_000022.11 22 33257731 + LARGE1 NNNTCAAGAATGAGCAGCTG NGG 2 0.3462 Tier II
10 NC_000008.11 8 138274713 - FAM135B NNNAAGAGAATGTGCAGCTG NGG 2 0.3401 Tier II
11 NC_000013.11 13 71573727 - DACH1 NNNAAAAGAATGTGCAGCTT NGG 2 0.3333 Tier II
12 NC_000007.14 7 72180062 - CALN1 NNNAAAAGAATGAGCAGCTG NGG 2 0.3297 Tier II
13 NC_000001.11 1 214659406 - CENPF NNNTAAAGAATTAGCAGCTG NGG 2 0.2663 Tier II
14 NC_000019.10 19 6844588 + VAV1 NNNTAAAAAATGTGCATCTG NGG 2 0.25 Tier II
15 NC_000001.11 1 223641012 + CAPN8 NNNTAAAGCATTTGCAGCTG NGG 2 0.2198 Tier II
16 NC_000020.11 20 59867360 - SYCP2 NNNTAAAGAATGAGCAGCTG NAG 2 0.1795 Tier II
17 NC_000005.10 5 107406615 - EFNA5 NNNTAGAGAATGTGAAGCTG NGG 2 0.1587 Tier II
18 NC_000012.12 12 172356 - IQSEC3 NNNTCAAGAATGGGCAGCTG NGG 2 0.1304 Tier II
19 NC_000011.10 11 21384251 - NELL1 NNNTAAAGAATGTGAAGCAG NGG 2 0.0635 Tier II
20 NC_000008.11 8 56209649 - PLAG1 NNNTAAAGAATGTTAAGCTG NGG 2 0.0593 Tier II
21 NC_000005.10 5 132616441 + RAD50 NNNTAAAGAATATGGAGCTG NGG 2 0.0467 Tier II
22 NC_000007.14 7 29976920 + SCRN1 NNNTACAGAATGTGTAGCTG NGG 2 0.0303 Tier II
23 NC_000013.11 13 23807418 + MIPEP NNNTAGAGAATGTGCAGCTG NTG 2 0.0278 Tier II
24 NC_000008.11 8 70182201 - NCOA2 NNNTAAAGAAAGTGCAGCTG NGC 2 0.0167 Tier II
25 NC_000020.11 20 34407567 - ITCH NNNTAAAGAATCTGCAGCTG NGC 2 0.0118 Tier II
26 NC_000023.11 X 6770581 - PUDP NNNTAGAGAATGTGCAGCTG NGT 2 0.0115 Tier II
27 NC_000023.11 X 38050555 + SYTL5 NNNTAAAGAATGTGAAGCTG NTG 2 0.0087 Tier II
28 NC_000016.10 16 49426872 - LOC105371244 NNNTAAGAAATGTGCAGCTG NGG 2 0.7059 Tier III
29 NC_000010.11 10 75343717 - ZNF503-AS1 NNNTAAAGAATCTGCAGCTA NGG 2 0.4963 Tier III
30 NC_000006.12 6 52598753 + LOC124901330 NNNAAAAGAATGTGCAGCTG NGG 1 0.4762 Tier III
31 NC_000006.12 6 17505332 - LOC101928491 NNNTAAATTATGTGCAGCTG NGG 2 0.375 Tier III
32 NC_000005.10 5 16426217 + LINC02150 NNNTAAAGAATGGGCAGCTG NGG 1 0.2609 Tier III
33 NC_000014.9 14 23941696 + DHRS4-AS1 NNNTAAAGAATAGGCAGCTG NGG 2 0.2435 Tier III
34 NC_000012.12 12 172356 - IQSEC3-AS1 NNNTCAAGAATGGGCAGCTG NGG 2 0.1304 Tier III
35 NC_000001.11 1 31571381 + LOC105378626 NNNTAACGAATGTGCAGCTG NAG 2 0.1134 Tier III
36 NC_000013.11 13 78186908 - OBI1-AS1 NNNTAAAGAATGGGCAGCAG NGG 2 0.0745 Tier III
37 NC_000001.11 1 88633039 + PKN2-AS1 NNNAAAAGAATGTGCAGCTG NTG 2 0.0186 Tier III
38 NC_000006.12 6 22696447 - LINC03005 NNNTAAAGAATATGCTGCTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 138515640 + Nek7 NNNTAAAGAATGTGCAGCTG NGG 0 1.0 Tier I
2 NC_000068.7 2 35284067 + Gsn NNNTAAAGACTGTGCAGCTG NGG 1 0.3333 Tier I
3 NC_000067.6 1 32414661 + Khdrbs2 NNNTAAAAATTGTGCAGCTG NGG 2 0.8824 Tier II
4 NC_000075.6 9 100692318 + Stag1 NNNTAAAGGTTGTGCAGCTG NGG 2 0.5882 Tier II
5 NC_000074.6 8 81402891 + Inpp4b NNNTAAAGAGTGTGCAACTG NGG 2 0.5185 Tier II
6 NC_000076.6 10 127555156 + Lrp1 NNNTAAGGGATGTGCAGCTG NGG 2 0.4706 Tier II
7 NC_000080.6 14 70193828 + Sorbs3 NNNCAGAGAATGTGCAGCTG NGG 2 0.4622 Tier II
8 NC_000078.6 12 74087277 - Syt16 NNNGAAAGAATGTGCAGATG NGG 2 0.3365 Tier II
9 NC_000076.6 10 3126681 - 9230019H11Rik NNNGAAAGAATCTGCAGCTG NGG 2 0.3309 Tier II
10 NC_000076.6 10 60434095 + Cdh23 NNNCACAGAATGTGCAGCTG NGG 2 0.2941 Tier II
11 NC_000080.6 14 101465010 - Tbc1d4 NNNTAAAGAATGTGCAACTG NAG 2 0.242 Tier II
12 NC_000070.6 4 9204593 - Clvs1 NNNTAAAGAAGGTGCAGATG NGG 2 0.2154 Tier II
13 NC_000068.7 2 181781937 - Myt1 NNNAAAAGAATGTGCAGCTC NGG 2 0.2041 Tier II
14 NC_000076.6 10 79871205 + Plppr3 NNNTAAAGAATTTGAAGCTG NGG 2 0.0855 Tier II
15 NC_000084.6 18 32958388 - Camk4 NNNTAAAGAATTTGAAGCTG NGG 2 0.0855 Tier II
16 NC_000080.6 14 117930566 + Gpc6 NNNTAAAGAATGTGAATCTG NGG 2 0.0556 Tier II
17 NC_000084.6 18 35308324 - Sil1 NNNAAAAGAATGTGCAGCTG NGC 2 0.0106 Tier II
18 NC_000074.6 8 90916152 - Chd9 NNNTAAAGAATGTTCAGCTG NGT 2 0.0043 Tier II
19 NC_000074.6 8 84396261 + Cacna1a NNNTAAAGAATGTGCTGCTG NAG 2 0.0 Tier II
20 NC_000085.6 19 22462863 - Trpm3 NNNTAAAGAATGTGCTGCTG NGC 2 0.0 Tier II
21 NC_000078.6 12 116742993 + Ptprn2 NNNTAAAGAATGTTCTGCTG NGG 2 0.0 Tier II
22 NC_000086.7 X 84108651 - Dmd NNNTAAAGAATGTTCTGCTG NGG 2 0.0 Tier II
23 NC_000072.6 6 98235662 - Gm32592 NNNTAAAACATGTGCAGCTG NGG 2 0.5714 Tier III
24 NC_000076.6 10 3126681 - B020014A21Rik NNNGAAAGAATCTGCAGCTG NGG 2 0.3309 Tier III
25 NC_000076.6 10 44605454 - Gm35154 NNNCAAAGACTGTGCAGCTG NGG 2 0.2157 Tier III
26 NC_000077.6 11 18488678 + Gm12022 NNNTAAAGAATGTGCAGCTT NAG 2 0.1815 Tier III
27 NC_000071.6 5 39088298 - Gm46937 NNNTAAAGAATGTGGAGCTT NGG 2 0.035 Tier III
28 NC_000078.6 12 116742993 + Gm31336 NNNTAAAGAATGTTCTGCTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)