Construct: sgRNA BRDN0001145542

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAGGTATTTGATTACCTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARK2 (2011)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77591

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 63898781 + MARK2 NNNGGTATTTGATTACCTAG NGG 0 1.0 Tier I
2 NC_000001.11 1 86974949 + HS2ST1 NNNGGAGTTTGATTACCTAG NGG 2 0.6118 Tier II
3 NC_000015.10 15 39872488 - GPR176 NNNGGTATCTCATTACCTAG NGG 2 0.3956 Tier II
4 NC_000023.11 X 71368454 + TAF1 NNNGGTATTTGAATACCTAC NGG 2 0.2967 Tier II
5 NC_000007.14 7 129051173 + TNPO3 NNNGGTATTTGATTACCAAC NGG 2 0.2857 Tier II
6 NC_000017.11 17 64475314 - MILR1 NNNAGTATTTGCTTACCTAG NGG 2 0.2368 Tier II
7 NC_000008.11 8 3825578 + CSMD1 NNNGGTGTTTGATTACCTAG NAG 2 0.183 Tier II
8 NC_000004.12 4 47686865 - CORIN NNNGGTATTTGATTCCCTAG NAG 2 0.0707 Tier II
9 NC_000002.12 2 115081945 - DPP10 NNNGGTATTTGATTTCCTAG NAG 2 0.0519 Tier II
10 NC_000003.12 3 33707421 - CLASP2 NNNGTTATTTGATTACCTAG NGA 2 0.0208 Tier II
11 NC_000019.10 19 30234267 + ZNF536 NNNGGTCTTTGATTACCTAG NTG 2 0.017 Tier II
12 NC_000020.11 20 58329029 + RAB22A NNNGGTATTTGTTTACCTAG NGT 2 0.0054 Tier II
13 NC_000003.12 3 79224849 - ROBO1 NNNGGTATTTGAGGACCTAG NGG 2 0.0 Tier II
14 NC_000008.11 8 3340667 + CSMD1 NNNGGTATTTGATGACCTTG NGG 2 0.0 Tier II
15 NC_000001.11 1 87371385 - LINC01364 NNNGGTATCTGAATACCTAG NGG 2 0.6391 Tier III
16 NC_000010.11 10 83314180 - MARK2P15 NNNGATGTTTGATTACCTAG NGG 2 0.6118 Tier III
17 NC_000001.11 1 86974949 + LOC124904211 NNNGGAGTTTGATTACCTAG NGG 2 0.6118 Tier III
18 NC_000007.14 7 36110588 - MARK2P13 NNNGGTATTTGAGTACCTAG NGG 1 0.2609 Tier III
19 NC_000002.12 2 115081945 - LOC105373575 NNNGGTATTTGATTTCCTAG NAG 2 0.0519 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 7286887 - Mark2 NNNGGTGTTTGATTACCTAG NGG 1 0.7059 Tier I
2 NC_000071.6 5 124483741 - Snrnp35 NNNTGTACTTGATTACCTAG NGG 2 0.3636 Tier II
3 NC_000074.6 8 38373229 + Sgcz NNNGGTATTTTATTACCTAA NGG 2 0.3606 Tier II
4 NC_000080.6 14 27354089 - Arhgef3 NNNAGTATTTGTTTACCTAG NGG 2 0.3 Tier II
5 NC_000068.7 2 71792383 - Itga6 NNNGGTGTTTGCTTACCTAG NGG 2 0.1858 Tier II
6 NC_000069.6 3 157187879 - Negr1 NNNGGTATTTGATTACCCAC NGG 2 0.1837 Tier II
7 NC_000069.6 3 69820197 + Sptssb NNNGTTATTTGTTTACCTAG NGG 2 0.1 Tier II
8 NC_000070.6 4 46715896 + Gabbr2 NNNGGTATTTTATTTCCTAG NGG 2 0.0769 Tier II
9 NC_000079.6 13 41319999 - Nedd9 NNNGGTATTTGCTTCCCTAG NGG 2 0.0718 Tier II
10 NC_000069.6 3 156947059 - Negr1 NNNGGTATTTGATTATCTAG NGT 2 0.005 Tier II
11 NC_000077.6 11 33269831 - Ranbp17 NNNGGTATTTGATGACCTAG NCG 2 0.0 Tier II
12 NC_000069.6 3 18414544 - Gm30667 NNNGGTATTTGATTACCAAG NGG 1 0.6667 Tier III
13 NC_000072.6 6 10956730 - Gm35822 NNNGATATTTCATTACCTAG NGG 2 0.3714 Tier III
14 NC_000071.6 5 124483741 - Gm52789 NNNTGTACTTGATTACCTAG NGG 2 0.3636 Tier III
Download CSV

Other clones with same target sequence:

(none)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.