Construct: sgRNA BRDN0001145542
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGGTATTTGATTACCTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MARK2 (2011)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77591
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
63898781 |
+ |
MARK2 |
NNNGGTATTTGATTACCTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
86974949 |
+ |
HS2ST1 |
NNNGGAGTTTGATTACCTAG |
NGG |
2 |
0.6118 |
Tier II |
| 3 |
NC_000015.10 |
15 |
39872488 |
- |
GPR176 |
NNNGGTATCTCATTACCTAG |
NGG |
2 |
0.3956 |
Tier II |
| 4 |
NC_000023.11 |
X |
71368454 |
+ |
TAF1 |
NNNGGTATTTGAATACCTAC |
NGG |
2 |
0.2967 |
Tier II |
| 5 |
NC_000007.14 |
7 |
129051173 |
+ |
TNPO3 |
NNNGGTATTTGATTACCAAC |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000017.11 |
17 |
64475314 |
- |
MILR1 |
NNNAGTATTTGCTTACCTAG |
NGG |
2 |
0.2368 |
Tier II |
| 7 |
NC_000008.11 |
8 |
3825578 |
+ |
CSMD1 |
NNNGGTGTTTGATTACCTAG |
NAG |
2 |
0.183 |
Tier II |
| 8 |
NC_000004.12 |
4 |
47686865 |
- |
CORIN |
NNNGGTATTTGATTCCCTAG |
NAG |
2 |
0.0707 |
Tier II |
| 9 |
NC_000002.12 |
2 |
115081945 |
- |
DPP10 |
NNNGGTATTTGATTTCCTAG |
NAG |
2 |
0.0519 |
Tier II |
| 10 |
NC_000003.12 |
3 |
33707421 |
- |
CLASP2 |
NNNGTTATTTGATTACCTAG |
NGA |
2 |
0.0208 |
Tier II |
| 11 |
NC_000019.10 |
19 |
30234267 |
+ |
ZNF536 |
NNNGGTCTTTGATTACCTAG |
NTG |
2 |
0.017 |
Tier II |
| 12 |
NC_000020.11 |
20 |
58329029 |
+ |
RAB22A |
NNNGGTATTTGTTTACCTAG |
NGT |
2 |
0.0054 |
Tier II |
| 13 |
NC_000003.12 |
3 |
79224849 |
- |
ROBO1 |
NNNGGTATTTGAGGACCTAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000008.11 |
8 |
3340667 |
+ |
CSMD1 |
NNNGGTATTTGATGACCTTG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000001.11 |
1 |
87371385 |
- |
LINC01364 |
NNNGGTATCTGAATACCTAG |
NGG |
2 |
0.6391 |
Tier III |
| 16 |
NC_000010.11 |
10 |
83314180 |
- |
MARK2P15 |
NNNGATGTTTGATTACCTAG |
NGG |
2 |
0.6118 |
Tier III |
| 17 |
NC_000001.11 |
1 |
86974949 |
+ |
LOC124904211 |
NNNGGAGTTTGATTACCTAG |
NGG |
2 |
0.6118 |
Tier III |
| 18 |
NC_000007.14 |
7 |
36110588 |
- |
MARK2P13 |
NNNGGTATTTGAGTACCTAG |
NGG |
1 |
0.2609 |
Tier III |
| 19 |
NC_000002.12 |
2 |
115081945 |
- |
LOC105373575 |
NNNGGTATTTGATTTCCTAG |
NAG |
2 |
0.0519 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
7286887 |
- |
Mark2 |
NNNGGTGTTTGATTACCTAG |
NGG |
1 |
0.7059 |
Tier I |
| 2 |
NC_000071.6 |
5 |
124483741 |
- |
Snrnp35 |
NNNTGTACTTGATTACCTAG |
NGG |
2 |
0.3636 |
Tier II |
| 3 |
NC_000074.6 |
8 |
38373229 |
+ |
Sgcz |
NNNGGTATTTTATTACCTAA |
NGG |
2 |
0.3606 |
Tier II |
| 4 |
NC_000080.6 |
14 |
27354089 |
- |
Arhgef3 |
NNNAGTATTTGTTTACCTAG |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000068.7 |
2 |
71792383 |
- |
Itga6 |
NNNGGTGTTTGCTTACCTAG |
NGG |
2 |
0.1858 |
Tier II |
| 6 |
NC_000069.6 |
3 |
157187879 |
- |
Negr1 |
NNNGGTATTTGATTACCCAC |
NGG |
2 |
0.1837 |
Tier II |
| 7 |
NC_000069.6 |
3 |
69820197 |
+ |
Sptssb |
NNNGTTATTTGTTTACCTAG |
NGG |
2 |
0.1 |
Tier II |
| 8 |
NC_000070.6 |
4 |
46715896 |
+ |
Gabbr2 |
NNNGGTATTTTATTTCCTAG |
NGG |
2 |
0.0769 |
Tier II |
| 9 |
NC_000079.6 |
13 |
41319999 |
- |
Nedd9 |
NNNGGTATTTGCTTCCCTAG |
NGG |
2 |
0.0718 |
Tier II |
| 10 |
NC_000069.6 |
3 |
156947059 |
- |
Negr1 |
NNNGGTATTTGATTATCTAG |
NGT |
2 |
0.005 |
Tier II |
| 11 |
NC_000077.6 |
11 |
33269831 |
- |
Ranbp17 |
NNNGGTATTTGATGACCTAG |
NCG |
2 |
0.0 |
Tier II |
| 12 |
NC_000069.6 |
3 |
18414544 |
- |
Gm30667 |
NNNGGTATTTGATTACCAAG |
NGG |
1 |
0.6667 |
Tier III |
| 13 |
NC_000072.6 |
6 |
10956730 |
- |
Gm35822 |
NNNGATATTTCATTACCTAG |
NGG |
2 |
0.3714 |
Tier III |
| 14 |
NC_000071.6 |
5 |
124483741 |
- |
Gm52789 |
NNNTGTACTTGATTACCTAG |
NGG |
2 |
0.3636 |
Tier III |
Other clones with same target sequence:
(none)
