Construct: sgRNA BRDN0001145556

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGCCAAAAATGTACTGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA4 (2043)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76665

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 221563815 - EPHA4 NNNGCCAAAAATGTACTGTG NGG 0 1.0 Tier I
2 NC_000011.10 11 67645289 - ACY3 NNNGCCAAAAATGGACTGGG NGG 2 0.0 Tier I
3 NC_000002.12 2 162196851 - FAP NNNGCAAAAAATATACTGTG NGG 2 0.8571 Tier II
4 NC_000023.11 X 12604082 - FRMPD4 NNNACCAAATATGTACTGTG NGG 2 0.7941 Tier II
5 NC_000005.10 5 150606481 - SYNPO NNNGCCAAAAACGAACTGTG NGG 2 0.5865 Tier II
6 NC_000016.10 16 83481802 + CDH13 NNNGACAGAAATGTACTGTG NGG 2 0.419 Tier II
7 NC_000015.10 15 35426986 + DPH6 NNNACCAAAAATGTACTGTC NGG 2 0.3857 Tier II
8 NC_000006.12 6 112138922 + LAMA4 NNNTCCAAAAATGTACTGTG NGG 1 0.3636 Tier II
9 NC_000010.11 10 51990097 - PRKG1 NNNGCCAAGAATGTACAGTG NGG 2 0.3556 Tier II
10 NC_000017.11 17 30456991 - CPD NNNGCCAAAAAAGTACTGTC NGG 2 0.3429 Tier II
11 NC_000012.12 12 101348472 - UTP20 NNNGACAACAATGTACTGTG NGG 2 0.3265 Tier II
12 NC_000008.11 8 17641589 + PDGFRL NNNGCCAAAAAGGTGCTGTG NGG 2 0.325 Tier II
13 NC_000017.11 17 66541926 - PRKCA NNNGCCAAAAATTTACTGTG NGG 1 0.3 Tier II
14 NC_000005.10 5 123162380 + PRDM6 NNNGCCAAAAATGTACTTTC NGG 2 0.2857 Tier II
15 NC_000023.11 X 74923235 - NEXMIF NNNGTCAAAAATTTACTGTG NGG 2 0.1909 Tier II
16 NC_000020.11 20 2920077 + PTPRA NNNGCCAAACATTTACTGTG NGG 2 0.1 Tier II
17 NC_000009.12 9 293384 - DOCK8 NNNGCTAAAAATGTACTGTG NGA 2 0.0645 Tier II
18 NC_000005.10 5 151779606 - G3BP1 NNNGCCAAATATGTACTGTG NGA 2 0.0613 Tier II
19 NC_000002.12 2 196538517 - HECW2 NNNCCCAAAAATGTACTGTG NGA 2 0.0368 Tier II
20 NC_000003.12 3 39896720 + MYRIP NNNGACAAAAATGTACTGTG NGT 2 0.0092 Tier II
21 NC_000017.11 17 2896171 - RAP1GAP2 NNNGCCAAACATGGACTGTG NGG 2 0.0 Tier II
22 NC_000015.10 15 97448267 + LINC02254 NNNGCCAGAAATGTACTATG NGG 2 0.5077 Tier III
23 NC_000015.10 15 35426986 + LOC124903464 NNNACCAAAAATGTACTGTC NGG 2 0.3857 Tier III
24 NC_000006.12 6 112138922 + LOC107986633 NNNTCCAAAAATGTACTGTG NGG 1 0.3636 Tier III
25 NC_000003.12 3 140601039 + LOC102724068 NNNGCCAAAAATGTACTGAG NGG 1 0.2857 Tier III
26 NC_000019.10 19 19824045 - ZNF56P NNNGCCAAAAATGTTCTGTG NGG 1 0.2 Tier III
27 NC_000019.10 19 20441925 + LOC105372317 NNNGCCAAAAATGTTCTGTG NGG 1 0.2 Tier III
28 NC_000019.10 19 20567313 - LOC105372316 NNNGCCAAAAATGTTCTGTG NGG 1 0.2 Tier III
29 NC_000019.10 19 20154115 - LOC105372310 NNNGCCAAAAATATTCTGTG NGG 2 0.1846 Tier III
30 NC_000008.11 8 33862179 + LOC105379364 NNNGCCAAACATGTACAGTG NGG 2 0.1778 Tier III
31 NC_000006.12 6 37527237 + LINC02520 NNNGCCAAAAGTGTACTGGG NGG 2 0.1625 Tier III
32 NC_000004.12 4 22339389 + LOC100505912 NNNGCCAAAAATGTTCTATG NGG 2 0.1385 Tier III
33 NC_000019.10 19 20432899 - LOC105372317 NNNGACAAAAATGTTCTGTG NGG 2 0.1143 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 77506632 - Epha4 NNNGCCAAAAATGTACTGTG NGG 0 1.0 Tier I
2 NC_000068.7 2 85923712 - Olfr1026 NNNGCCGTAAATGTACTGTG NGG 2 0.3025 Tier I
3 NC_000072.6 6 127019906 + Fgf6 NNNGCCAGAAATGTACTGTG NGG 1 0.7333 Tier II
4 NC_000074.6 8 16175360 + Csmd1 NNNCCTAAAAATGTACTGTG NGG 2 0.4916 Tier II
5 NC_000067.6 1 161999896 - Dnm3 NNNGGCAAAAAAGTACTGTG NGG 2 0.48 Tier II
6 NC_000079.6 13 62026112 - Gm3325 NNNGCCAAAAATGAACTTTG NGG 2 0.4127 Tier II
7 NC_000069.6 3 60620755 - Mbnl1 NNNGCACAAAATGTACTGTG NGG 2 0.4062 Tier II
8 NC_000072.6 6 100607647 - Shq1 NNNGCTTAAAATGTACTGTG NGG 2 0.4062 Tier II
9 NC_000080.6 14 18795535 + Ube2e2 NNNGCCAAAAATGAACTGTC NGG 2 0.2653 Tier II
10 NC_000067.6 1 4871917 + Tcea1 NNNGCCAAAAATGTACACTG NGG 2 0.254 Tier II
11 NC_000067.6 1 21431502 + Kcnq5 NNNGCCAAGAATGTACTGAG NGG 2 0.1905 Tier II
12 NC_000084.6 18 42665412 + Ppp2r2b NNNGCCAAAAATGTACTTTG NAG 2 0.1728 Tier II
13 NC_000085.6 19 4576311 - Pcx NNNGACAAAAATGTACTGTG NAG 2 0.1481 Tier II
14 NC_000079.6 13 60737486 - Dapk1 NNNGCCTAAAATGTCCTGTG NGG 2 0.1193 Tier II
15 NC_000072.6 6 116093851 - Tmcc1 NNNGCCAAAAATGCATTGTG NGG 2 0.0879 Tier II
16 NC_000070.6 4 102832849 + Sgip1 NNNGACAAAAATGTACTGTG NGA 2 0.0397 Tier II
17 NC_000067.6 1 99872917 - Cntnap5b NNNGCCAAAAATGTATTGTG NGA 2 0.0214 Tier II
18 NC_000078.6 12 56999300 - Slc25a21 NNNGCCAAAAATGTCCTGTG NGT 2 0.0044 Tier II
19 NC_000079.6 13 62141870 - Zfp808 NNNGCCAAAAATGGACTTTG NGG 2 0.0 Tier II
20 NC_000079.6 13 62538665 + Zfp934 NNNGCCAAAAATGGACTTTG NGG 2 0.0 Tier II
21 NC_000068.7 2 12144038 - Itga8 NNNGCCAAAAATGTACTGTG NCA 2 0.0 Tier II
22 NC_000076.6 10 54351702 + Gm36335 NNNTCCAAATATGTACTGTG NGG 2 0.3209 Tier III
23 NC_000084.6 18 68902845 - 4930546C10Rik NNNGCCAAAAGTGTCCTGTG NGG 2 0.1773 Tier III
24 NC_000070.6 4 112561290 - Gm12814 NNNGCCAAAAATTTACTGTG NGA 2 0.0208 Tier III
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Other clones with same target sequence:

(none)