Construct: sgRNA BRDN0001145558
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCAGAGCTTGACACAGCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK2B (816)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77743
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
44284202 |
+ |
CAMK2B |
NNNAGAGCTTGACACAGCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
2944638 |
- |
CARD11 |
NNNAGAGCTTGGCACAACGT |
NGG |
2 |
0.6741 |
Tier II |
| 3 |
NC_000004.12 |
4 |
65637704 |
- |
EPHA5 |
NNNAGAACTTGACACAGTGT |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000003.12 |
3 |
123900114 |
+ |
CCDC14 |
NNNAGAGCTTGACACAATGT |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000006.12 |
6 |
136539177 |
- |
MAP7 |
NNNAGTGCTTGACACAGCAT |
NGG |
2 |
0.5102 |
Tier II |
| 6 |
NC_000003.12 |
3 |
124580741 |
+ |
KALRN |
NNNAGAGCCTGACACAGAGT |
NGG |
2 |
0.497 |
Tier II |
| 7 |
NC_000017.11 |
17 |
79602305 |
+ |
RBFOX3 |
NNNAAAGCTCGACACAGCGT |
NGG |
2 |
0.4622 |
Tier II |
| 8 |
NC_000014.9 |
14 |
74899819 |
- |
DLST |
NNNAGAGCTTGATACAGTGT |
NGG |
2 |
0.45 |
Tier II |
| 9 |
NC_000022.11 |
22 |
39931138 |
+ |
GRAP2 |
NNNAGGGCTTGACACAGAGT |
NGG |
2 |
0.3846 |
Tier II |
| 10 |
NC_000003.12 |
3 |
179820419 |
- |
PEX5L |
NNNAGTGCTTGACACAGAGT |
NGG |
2 |
0.3846 |
Tier II |
| 11 |
NC_000004.12 |
4 |
77589717 |
+ |
CXCL13 |
NNNAGTGCTTGACACAGAGT |
NGG |
2 |
0.3846 |
Tier II |
| 12 |
NC_000023.11 |
X |
111338537 |
+ |
DCX |
NNNAGAGGTTGACACAGAGT |
NGG |
2 |
0.3462 |
Tier II |
| 13 |
NC_000013.11 |
13 |
108586054 |
+ |
MYO16 |
NNNAGATCTTGACACAGAGT |
NGG |
2 |
0.3077 |
Tier II |
| 14 |
NC_000009.12 |
9 |
126920153 |
+ |
RALGPS1 |
NNNAGAGCTTGACACAGGGT |
NGG |
1 |
0.1333 |
Tier II |
| 15 |
NC_000022.11 |
22 |
43841247 |
+ |
SULT4A1 |
NNNAGAGCATGACACAGGGT |
NGG |
2 |
0.1238 |
Tier II |
| 16 |
NC_000001.11 |
1 |
84880662 |
+ |
LPAR3 |
NNNAGAGCTTGACCCAGAGT |
NGG |
2 |
0.1154 |
Tier II |
| 17 |
NC_000006.12 |
6 |
21210158 |
- |
CDKAL1 |
NNNAGTGCTTGACACAGCGC |
NGG |
2 |
0.0649 |
Tier II |
| 18 |
NC_000011.10 |
11 |
78723431 |
+ |
TENM4 |
NNNAGAGCTTGACACAGAGT |
NGA |
2 |
0.0374 |
Tier II |
| 19 |
NC_000006.12 |
6 |
16311131 |
- |
ATXN1 |
NNNAGAGCTTGACAGAGAGT |
NGG |
2 |
0.0269 |
Tier II |
| 20 |
NC_000016.10 |
16 |
23839842 |
+ |
PRKCB |
NNNAGAGCTTGACACAGAGT |
NTG |
2 |
0.021 |
Tier II |
| 21 |
NC_000002.12 |
2 |
68734132 |
- |
ARHGAP25 |
NNNAGAGCTTGACACAGAGT |
NGT |
2 |
0.0087 |
Tier II |
| 22 |
NC_000007.14 |
7 |
2944638 |
- |
CARD11-AS1 |
NNNAGAGCTTGGCACAACGT |
NGG |
2 |
0.6741 |
Tier III |
| 23 |
NC_000003.12 |
3 |
124580741 |
+ |
LOC105374076 |
NNNAGAGCCTGACACAGAGT |
NGG |
2 |
0.497 |
Tier III |
| 24 |
NC_000019.10 |
19 |
50065988 |
- |
ZNF473CR |
NNNAGAGCTTGGCACAGAGT |
NGG |
2 |
0.3889 |
Tier III |
| 25 |
NC_000004.12 |
4 |
77589717 |
+ |
LOC105377296 |
NNNAGTGCTTGACACAGAGT |
NGG |
2 |
0.3846 |
Tier III |
| 26 |
NC_000006.12 |
6 |
19789558 |
- |
LNC-LBCS |
NNNAGAGCTTGACACAGCGC |
NAG |
2 |
0.0236 |
Tier III |
| 27 |
NC_000005.10 |
5 |
13537155 |
- |
LOC105374660 |
NNNAGAGCTTGAGACAGCGT |
NTG |
2 |
0.0053 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
6028938 |
+ |
Camk2b |
NNNAGAGCTTGACACAGCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000083.6 |
17 |
45592354 |
+ |
Slc29a1 |
NNNAGAGCTGGACACAGAGT |
NGG |
2 |
0.2692 |
Tier II |
| 3 |
NC_000082.6 |
16 |
4940840 |
+ |
Nudt16l1 |
NNNAGAGCCTGACCCAGCGT |
NGG |
2 |
0.1978 |
Tier II |
| 4 |
NC_000082.6 |
16 |
33418095 |
+ |
Zfp148 |
NNNAGAGCTTGAAGCAGCGT |
NGG |
2 |
0.1795 |
Tier II |
| 5 |
NC_000075.6 |
9 |
24303564 |
- |
Npsr1 |
NNNAGAGTTTGACACAGCGT |
NGA |
2 |
0.0608 |
Tier II |
| 6 |
NC_000073.6 |
7 |
35212029 |
- |
Lrp3 |
NNNAGAGGTTGACACAGCGT |
NTG |
2 |
0.025 |
Tier II |
| 7 |
NC_000075.6 |
9 |
115128901 |
- |
Osbpl10 |
NNNAGAGCTTGACACCTCGT |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
