Construct: sgRNA BRDN0001145599

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTGACTATCCTTTCAAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAR2A (5576)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77673

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 48783062 + PRKAR2A NNNGACTATCCTTTCAAACA NGG 0 1.0 Tier I
2 NC_000002.12 2 152090218 - CACNB4 NNNGACTGCCCTTTCAAACA NGG 2 0.6769 Tier II
3 NC_000008.11 8 138634255 + COL22A1 NNNCACTATACTTTCAAACA NGG 2 0.4588 Tier II
4 NC_000016.10 16 54119286 + FTO NNNGATTATCCTTTCAAATA NGG 2 0.4286 Tier II
5 NC_000001.11 1 176657457 - PAPPA2 NNNGACTAACCTTTCAAAAA NGG 2 0.398 Tier II
6 NC_000023.11 X 11947640 - FRMPD4 NNNTACTGTCCTTTCAAACA NGG 2 0.2667 Tier II
7 NC_000017.11 17 43541528 + DHX8 NNNGACTCTCCTTTCAAAAA NGG 2 0.1837 Tier II
8 NC_000017.11 17 43541528 + ETV4 NNNGACTCTCCTTTCAAAAA NGG 2 0.1837 Tier II
9 NC_000001.11 1 145934386 + LIX1L NNNGACCATCCTGTCAAACA NGG 2 0.1793 Tier II
10 NC_000009.12 9 34841672 + PHF24 NNNGACTAGCCTGTCAAACA NGG 2 0.1615 Tier II
11 NC_000015.10 15 34165611 + KATNBL1 NNNGACTAGCCTGTCAAACA NGG 2 0.1615 Tier II
12 NC_000004.12 4 144669954 + HHIP NNNGACTATCCTGACAAACA NGG 2 0.1615 Tier II
13 NC_000017.11 17 48209584 - SKAP1 NNNGAATATCCTTTCATACA NGG 2 0.1238 Tier II
14 NC_000004.12 4 99058626 - METAP1 NNNGAGTATCCTTTAAAACA NGG 2 0.1111 Tier II
15 NC_000010.11 10 79180776 + ZMIZ1 NNNGACTTTCCTTTCAGACA NGG 2 0.0756 Tier II
16 NC_000009.12 9 95495408 + PTCH1 NNNGACTATCCTTTCAAACA NGA 1 0.0694 Tier II
17 NC_000022.11 22 29101904 + KREMEN1 NNNGACTATACTTTTAAACA NGG 2 0.0578 Tier II
18 NC_000001.11 1 6119304 - CHD5 NNNGACTATCCTGTCGAACA NGG 2 0.0502 Tier II
19 NC_000003.12 3 173029682 - SPATA16 NNNGACTATCTTTTCAAAGA NGG 2 0.0385 Tier II
20 NC_000001.11 1 100047384 + MFSD14A NNNGACTATCCTTTCATACC NGG 2 0.0303 Tier II
21 NC_000016.10 16 73844117 + ZFHX3 NNNGACTATACTTTCAAACA NGT 2 0.014 Tier II
22 NC_000003.12 3 85850534 - CADM2 NNNGACCATCCTTGCAAACA NGG 2 0.0 Tier II
23 NC_000019.10 19 58411848 - ZNF584 NNNGACCATCCTTGCAAACA NGG 2 0.0 Tier II
24 NC_000010.11 10 17141366 - TRDMT1 NNNGACCATCCTTTCCAACA NGG 2 0.0 Tier II
25 NC_000001.11 1 102967446 - COL11A1 NNNGACCATCCTTTCTAACA NGG 2 0.0 Tier II
26 NC_000002.12 2 28939920 + WDR43 NNNGACCATCCTTTCTAACA NGG 2 0.0 Tier II
27 NC_000017.11 17 48838527 + CALCOCO2 NNNGACCATCCTTTCTAACA NGG 2 0.0 Tier II
28 NC_000003.12 3 130923682 + ATP2C1 NNNGACTAGCCTTGCAAACA NGG 2 0.0 Tier II
29 NC_000004.12 4 15991747 + PROM1 NNNGACTATCCTGGCAAACA NGG 2 0.0 Tier II
30 NC_000012.12 12 121777176 - TMEM120B NNNGACTATCCTGGCAAACA NGG 2 0.0 Tier II
31 NC_000012.12 12 132757635 + ANKLE2 NNNGACTATCCTGGCAAACA NGG 2 0.0 Tier II
32 NC_000016.10 16 2718523 - PRSS27 NNNGACTATCCTGGCAAACA NGG 2 0.0 Tier II
33 NC_000017.11 17 1771444 - SERPINF1 NNNGACTATCCTGGCAAACA NGG 2 0.0 Tier II
34 NC_000017.11 17 78009759 - TNRC6C NNNGACTATCCTGGCAAACA NGG 2 0.0 Tier II
35 NC_000004.12 4 152939340 - FHDC1 NNNGACTATCCTGTCCAACA NGG 2 0.0 Tier II
36 NC_000008.11 8 19019001 - PSD3 NNNGACTATCCTGTCCAACA NGG 2 0.0 Tier II
37 NC_000015.10 15 90732908 + BLM NNNGACTATCCTGTCCAACA NGG 2 0.0 Tier II
38 NC_000016.10 16 14568708 + PARN NNNGACTATCCTGTCCAACA NGG 2 0.0 Tier II
39 NC_000002.12 2 14874439 + NBAS NNNGACTATCCTGTCTAACA NGG 2 0.0 Tier II
40 NC_000005.10 5 177012006 - UIMC1 NNNGACTATCCTGTCTAACA NGG 2 0.0 Tier II
41 NC_000016.10 16 24706524 + TNRC6A NNNGACTATCCTGTCTAACA NGG 2 0.0 Tier II
42 NC_000019.10 19 47133431 - SAE1 NNNGACTATCCTGTCTAACA NGG 2 0.0 Tier II
43 NC_000010.11 10 73776885 - FUT11 NNNGACTATCCTTGCCAACA NGG 2 0.0 Tier II
44 NC_000018.10 18 63594653 + SERPINB13 NNNGACTATCCTTGCCAACA NGG 2 0.0 Tier II
45 NC_000007.14 7 152389088 - KMT2C NNNGACTATCCTTGCTAACA NGG 2 0.0 Tier II
46 NC_000011.10 11 47322420 + MADD NNNGACTATCCTTGCTAACA NGG 2 0.0 Tier II
47 NC_000008.11 8 11090454 - XKR6 NNNGACTATCCTTTCCAACA NTG 2 0.0 Tier II
48 NC_000006.12 6 74728915 + LOC105377858 NNNGATTATCATTTCAAACA NGG 2 0.6964 Tier III
49 NC_000001.11 1 145934386 + LIX1L-AS1 NNNGACCATCCTGTCAAACA NGG 2 0.1793 Tier III
50 NC_000020.11 20 10091644 - SNAP25-AS1 NNNGACTTTCCTTTCAACCA NGG 2 0.0816 Tier III
51 NC_000002.12 2 239792449 - LOC150935 NNNGACTCTCCTTTCAGACA NGG 2 0.0756 Tier III
52 NC_000013.11 13 45936451 - LOC105370191 NNNGACTAACCTTTCAAACA NGC 2 0.0206 Tier III
53 NC_000022.11 22 43815513 - EFCAB6-DT NNNGACTATCCTTTAAAACA NTG 2 0.0087 Tier III
54 NC_000003.12 3 754050 - LINC01266 NNNGACCATCCTTGCAAACA NGG 2 0.0 Tier III
55 NC_000014.9 14 22054520 + TRA NNNGACCATCCTTGCAAACA NGG 2 0.0 Tier III
56 NC_000010.11 10 9863411 + LINC02663 NNNGACTAGCCTTTCCAACA NGG 2 0.0 Tier III
57 NC_000001.11 1 199198169 + LINC02789 NNNGACTATCCTGGCAAACA NGG 2 0.0 Tier III
58 NC_000022.11 22 30913254 + LOC107985544 NNNGACTATCCTGGCAAACA NGG 2 0.0 Tier III
59 NC_000008.11 8 19019001 - LOC124901898 NNNGACTATCCTGTCCAACA NGG 2 0.0 Tier III
60 NC_000016.10 16 2376945 + ABCA17P NNNGACTATCCTGTCCAACA NGG 2 0.0 Tier III
61 NC_000005.10 5 58549527 + LINC02108 NNNGACTATCCTGTCTAACA NGG 2 0.0 Tier III
62 NC_000007.14 7 136164552 + LOC105375523 NNNGACTATCCTGTCTAACA NGG 2 0.0 Tier III
63 NC_000017.11 17 6946469 + LOC124903906 NNNGACTATCCTGTCTAACA NGG 2 0.0 Tier III
64 NC_000008.11 8 48607925 - LOC101929268 NNNGACTATCCTTCCTAACA NGG 2 0.0 Tier III
65 NC_000008.11 8 48607925 - LINC02847 NNNGACTATCCTTCCTAACA NGG 2 0.0 Tier III
66 NC_000023.11 X 149997506 - EOLA2-DT NNNGACTATCCTTGCCAACA NGG 2 0.0 Tier III
67 NC_000005.10 5 112925050 + LOC105379123 NNNGACTATCCTTGCTAACA NGG 2 0.0 Tier III
68 NC_000006.12 6 114507313 - LNCPOIR NNNGACTATCCTTGCTAACA NGG 2 0.0 Tier III
69 NC_000023.11 X 24093308 + LOC124905260 NNNGACTATCCTTGCTAACA NGG 2 0.0 Tier III
70 NC_000023.11 X 24093308 + LOC124905261 NNNGACTATCCTTGCTAACA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 90807993 + Col6a3 NNNGACTATCCTCTCAATCA NGG 2 0.3947 Tier I
2 NC_000075.6 9 108728205 - Prkar2a NNNGACAATCTTTTCAAACA NGG 2 0.2692 Tier I
3 NC_000069.6 3 68268995 - Schip1 NNNAACTATTCTTTCAAACA NGG 2 0.8471 Tier II
4 NC_000069.6 3 68268995 - Iqschfp NNNAACTATTCTTTCAAACA NGG 2 0.8471 Tier II
5 NC_000074.6 8 111386704 - Fa2h NNNGGCTATCCTTTCAAACA NGG 1 0.72 Tier II
6 NC_000086.7 X 93656775 + Pcyt1b NNNAACTATCCTATCAAACA NGG 2 0.6231 Tier II
7 NC_000079.6 13 63553977 + Ptch1 NNNGGCTGTCCTTTCAAACA NGG 2 0.528 Tier II
8 NC_000070.6 4 111821016 - Spata6 NNNGACAATCCTTTCAAAAA NGG 2 0.375 Tier II
9 NC_000068.7 2 50089284 + Lypd6 NNNGACATTCCTTTCAAACA NGG 2 0.375 Tier II
10 NC_000081.6 15 40686397 - Zfpm2 NNNGACATTCCTTTCAAACA NGG 2 0.375 Tier II
11 NC_000068.7 2 49141504 + Mbd5 NNNGACTTTACTTTCAAACA NGG 2 0.3714 Tier II
12 NC_000078.6 12 99298366 + Foxn3 NNNGACTTTACTTTCAAACA NGG 2 0.3714 Tier II
13 NC_000080.6 14 115400175 + Gpc5 NNNGACTTTACTTTCAAACA NGG 2 0.3714 Tier II
14 NC_000081.6 15 37577853 + Ncald NNNGTTTATCCTTTCAAACA NGG 2 0.3377 Tier II
15 NC_000067.6 1 84484857 + Dner NNNGACTATCCATTCAAGCA NGG 2 0.32 Tier II
16 NC_000069.6 3 127351040 - Ank2 NNNGACTATTCTTTCAAACA NAG 2 0.244 Tier II
17 NC_000067.6 1 135990411 - Igfn1 NNNGACTATCTTTTCAATCA NGG 2 0.1538 Tier II
18 NC_000067.6 1 124483100 - Dpp10 NNNGACTATACTTTCACACA NGG 2 0.1529 Tier II
19 NC_000077.6 11 48976129 + 9930111J21Rik1 NNNGACTTTCCTTTAAAACA NGG 2 0.0952 Tier II
20 NC_000077.6 11 49047970 + 9930111J21Rik2 NNNGACTTTCCTTTAAAACA NGG 2 0.0952 Tier II
21 NC_000075.6 9 15529994 + Smco4 NNNGACTATACTTTCAAACA NGA 2 0.0602 Tier II
22 NC_000068.7 2 12974888 + Pter NNNGACTATCCTTTCACACA NGA 2 0.0123 Tier II
23 NC_000070.6 4 66208882 - Astn2 NNNGACTATCCTTTGAAACA NGC 2 0.0011 Tier II
24 NC_000079.6 13 32196061 + Gmds NNNGACTATCCTTTATAACA NGG 2 0.0 Tier II
25 NC_000067.6 1 71622112 - Fn1 NNNGAGTATCCTTTCCAACA NGG 2 0.0 Tier II
26 NC_000083.6 17 60946865 - Gm33890 NNNGACGATACTTTCAAACA NGG 2 0.5098 Tier III
27 NC_000067.6 1 69434919 - Gm28497 NNNGAATATCCTTTCAAATA NGG 2 0.4286 Tier III
28 NC_000080.6 14 115400175 + 4930505G20Rik NNNGACTTTACTTTCAAACA NGG 2 0.3714 Tier III
29 NC_000068.7 2 75154403 - 2600014E21Rik NNNGACTCTCCTTTCAAACT NGG 2 0.2571 Tier III
30 NC_000076.6 10 25651965 + Gm29571 NNNGACTTTCCTTTCAAACC NGG 2 0.0974 Tier III
31 NC_000075.6 9 15529994 + Gm51703 NNNGACTATACTTTCAAACA NGA 2 0.0602 Tier III
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Other clones with same target sequence:

(none)