Construct: sgRNA BRDN0001145599
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGACTATCCTTTCAAACA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PRKAR2A (5576)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000003.12 | 3 | 48783062 | + | PRKAR2A | NNNGACTATCCTTTCAAACA | NGG | 0 | 1.0 | Tier I |
2 | NC_000002.12 | 2 | 152090218 | - | CACNB4 | NNNGACTGCCCTTTCAAACA | NGG | 2 | 0.6769 | Tier II |
3 | NC_000008.11 | 8 | 138634255 | + | COL22A1 | NNNCACTATACTTTCAAACA | NGG | 2 | 0.4588 | Tier II |
4 | NC_000016.10 | 16 | 54119286 | + | FTO | NNNGATTATCCTTTCAAATA | NGG | 2 | 0.4286 | Tier II |
5 | NC_000001.11 | 1 | 176657457 | - | PAPPA2 | NNNGACTAACCTTTCAAAAA | NGG | 2 | 0.398 | Tier II |
6 | NC_000023.11 | X | 11947640 | - | FRMPD4 | NNNTACTGTCCTTTCAAACA | NGG | 2 | 0.2667 | Tier II |
7 | NC_000017.11 | 17 | 43541528 | + | DHX8 | NNNGACTCTCCTTTCAAAAA | NGG | 2 | 0.1837 | Tier II |
8 | NC_000017.11 | 17 | 43541528 | + | ETV4 | NNNGACTCTCCTTTCAAAAA | NGG | 2 | 0.1837 | Tier II |
9 | NC_000001.11 | 1 | 145934386 | + | LIX1L | NNNGACCATCCTGTCAAACA | NGG | 2 | 0.1793 | Tier II |
10 | NC_000009.12 | 9 | 34841672 | + | PHF24 | NNNGACTAGCCTGTCAAACA | NGG | 2 | 0.1615 | Tier II |
11 | NC_000015.10 | 15 | 34165611 | + | KATNBL1 | NNNGACTAGCCTGTCAAACA | NGG | 2 | 0.1615 | Tier II |
12 | NC_000004.12 | 4 | 144669954 | + | HHIP | NNNGACTATCCTGACAAACA | NGG | 2 | 0.1615 | Tier II |
13 | NC_000017.11 | 17 | 48209584 | - | SKAP1 | NNNGAATATCCTTTCATACA | NGG | 2 | 0.1238 | Tier II |
14 | NC_000004.12 | 4 | 99058626 | - | METAP1 | NNNGAGTATCCTTTAAAACA | NGG | 2 | 0.1111 | Tier II |
15 | NC_000010.11 | 10 | 79180776 | + | ZMIZ1 | NNNGACTTTCCTTTCAGACA | NGG | 2 | 0.0756 | Tier II |
16 | NC_000009.12 | 9 | 95495408 | + | PTCH1 | NNNGACTATCCTTTCAAACA | NGA | 1 | 0.0694 | Tier II |
17 | NC_000022.11 | 22 | 29101904 | + | KREMEN1 | NNNGACTATACTTTTAAACA | NGG | 2 | 0.0578 | Tier II |
18 | NC_000001.11 | 1 | 6119304 | - | CHD5 | NNNGACTATCCTGTCGAACA | NGG | 2 | 0.0502 | Tier II |
19 | NC_000003.12 | 3 | 173029682 | - | SPATA16 | NNNGACTATCTTTTCAAAGA | NGG | 2 | 0.0385 | Tier II |
20 | NC_000001.11 | 1 | 100047384 | + | MFSD14A | NNNGACTATCCTTTCATACC | NGG | 2 | 0.0303 | Tier II |
21 | NC_000016.10 | 16 | 73844117 | + | ZFHX3 | NNNGACTATACTTTCAAACA | NGT | 2 | 0.014 | Tier II |
22 | NC_000003.12 | 3 | 85850534 | - | CADM2 | NNNGACCATCCTTGCAAACA | NGG | 2 | 0.0 | Tier II |
23 | NC_000019.10 | 19 | 58411848 | - | ZNF584 | NNNGACCATCCTTGCAAACA | NGG | 2 | 0.0 | Tier II |
24 | NC_000010.11 | 10 | 17141366 | - | TRDMT1 | NNNGACCATCCTTTCCAACA | NGG | 2 | 0.0 | Tier II |
25 | NC_000001.11 | 1 | 102967446 | - | COL11A1 | NNNGACCATCCTTTCTAACA | NGG | 2 | 0.0 | Tier II |
26 | NC_000002.12 | 2 | 28939920 | + | WDR43 | NNNGACCATCCTTTCTAACA | NGG | 2 | 0.0 | Tier II |
27 | NC_000017.11 | 17 | 48838527 | + | CALCOCO2 | NNNGACCATCCTTTCTAACA | NGG | 2 | 0.0 | Tier II |
28 | NC_000003.12 | 3 | 130923682 | + | ATP2C1 | NNNGACTAGCCTTGCAAACA | NGG | 2 | 0.0 | Tier II |
29 | NC_000004.12 | 4 | 15991747 | + | PROM1 | NNNGACTATCCTGGCAAACA | NGG | 2 | 0.0 | Tier II |
30 | NC_000012.12 | 12 | 121777176 | - | TMEM120B | NNNGACTATCCTGGCAAACA | NGG | 2 | 0.0 | Tier II |
31 | NC_000012.12 | 12 | 132757635 | + | ANKLE2 | NNNGACTATCCTGGCAAACA | NGG | 2 | 0.0 | Tier II |
32 | NC_000016.10 | 16 | 2718523 | - | PRSS27 | NNNGACTATCCTGGCAAACA | NGG | 2 | 0.0 | Tier II |
33 | NC_000017.11 | 17 | 1771444 | - | SERPINF1 | NNNGACTATCCTGGCAAACA | NGG | 2 | 0.0 | Tier II |
34 | NC_000017.11 | 17 | 78009759 | - | TNRC6C | NNNGACTATCCTGGCAAACA | NGG | 2 | 0.0 | Tier II |
35 | NC_000004.12 | 4 | 152939340 | - | FHDC1 | NNNGACTATCCTGTCCAACA | NGG | 2 | 0.0 | Tier II |
36 | NC_000008.11 | 8 | 19019001 | - | PSD3 | NNNGACTATCCTGTCCAACA | NGG | 2 | 0.0 | Tier II |
37 | NC_000015.10 | 15 | 90732908 | + | BLM | NNNGACTATCCTGTCCAACA | NGG | 2 | 0.0 | Tier II |
38 | NC_000016.10 | 16 | 14568708 | + | PARN | NNNGACTATCCTGTCCAACA | NGG | 2 | 0.0 | Tier II |
39 | NC_000002.12 | 2 | 14874439 | + | NBAS | NNNGACTATCCTGTCTAACA | NGG | 2 | 0.0 | Tier II |
40 | NC_000005.10 | 5 | 177012006 | - | UIMC1 | NNNGACTATCCTGTCTAACA | NGG | 2 | 0.0 | Tier II |
41 | NC_000016.10 | 16 | 24706524 | + | TNRC6A | NNNGACTATCCTGTCTAACA | NGG | 2 | 0.0 | Tier II |
42 | NC_000019.10 | 19 | 47133431 | - | SAE1 | NNNGACTATCCTGTCTAACA | NGG | 2 | 0.0 | Tier II |
43 | NC_000010.11 | 10 | 73776885 | - | FUT11 | NNNGACTATCCTTGCCAACA | NGG | 2 | 0.0 | Tier II |
44 | NC_000018.10 | 18 | 63594653 | + | SERPINB13 | NNNGACTATCCTTGCCAACA | NGG | 2 | 0.0 | Tier II |
45 | NC_000007.14 | 7 | 152389088 | - | KMT2C | NNNGACTATCCTTGCTAACA | NGG | 2 | 0.0 | Tier II |
46 | NC_000011.10 | 11 | 47322420 | + | MADD | NNNGACTATCCTTGCTAACA | NGG | 2 | 0.0 | Tier II |
47 | NC_000008.11 | 8 | 11090454 | - | XKR6 | NNNGACTATCCTTTCCAACA | NTG | 2 | 0.0 | Tier II |
48 | NC_000006.12 | 6 | 74728915 | + | LOC105377858 | NNNGATTATCATTTCAAACA | NGG | 2 | 0.6964 | Tier III |
49 | NC_000001.11 | 1 | 145934386 | + | LIX1L-AS1 | NNNGACCATCCTGTCAAACA | NGG | 2 | 0.1793 | Tier III |
50 | NC_000020.11 | 20 | 10091644 | - | SNAP25-AS1 | NNNGACTTTCCTTTCAACCA | NGG | 2 | 0.0816 | Tier III |
51 | NC_000002.12 | 2 | 239792449 | - | LOC150935 | NNNGACTCTCCTTTCAGACA | NGG | 2 | 0.0756 | Tier III |
52 | NC_000013.11 | 13 | 45936451 | - | LOC105370191 | NNNGACTAACCTTTCAAACA | NGC | 2 | 0.0206 | Tier III |
53 | NC_000022.11 | 22 | 43815513 | - | EFCAB6-DT | NNNGACTATCCTTTAAAACA | NTG | 2 | 0.0087 | Tier III |
54 | NC_000003.12 | 3 | 754050 | - | LINC01266 | NNNGACCATCCTTGCAAACA | NGG | 2 | 0.0 | Tier III |
55 | NC_000014.9 | 14 | 22054520 | + | TRA | NNNGACCATCCTTGCAAACA | NGG | 2 | 0.0 | Tier III |
56 | NC_000010.11 | 10 | 9863411 | + | LINC02663 | NNNGACTAGCCTTTCCAACA | NGG | 2 | 0.0 | Tier III |
57 | NC_000001.11 | 1 | 199198169 | + | LINC02789 | NNNGACTATCCTGGCAAACA | NGG | 2 | 0.0 | Tier III |
58 | NC_000022.11 | 22 | 30913254 | + | LOC107985544 | NNNGACTATCCTGGCAAACA | NGG | 2 | 0.0 | Tier III |
59 | NC_000008.11 | 8 | 19019001 | - | LOC124901898 | NNNGACTATCCTGTCCAACA | NGG | 2 | 0.0 | Tier III |
60 | NC_000016.10 | 16 | 2376945 | + | ABCA17P | NNNGACTATCCTGTCCAACA | NGG | 2 | 0.0 | Tier III |
61 | NC_000005.10 | 5 | 58549527 | + | LINC02108 | NNNGACTATCCTGTCTAACA | NGG | 2 | 0.0 | Tier III |
62 | NC_000007.14 | 7 | 136164552 | + | LOC105375523 | NNNGACTATCCTGTCTAACA | NGG | 2 | 0.0 | Tier III |
63 | NC_000017.11 | 17 | 6946469 | + | LOC124903906 | NNNGACTATCCTGTCTAACA | NGG | 2 | 0.0 | Tier III |
64 | NC_000008.11 | 8 | 48607925 | - | LOC101929268 | NNNGACTATCCTTCCTAACA | NGG | 2 | 0.0 | Tier III |
65 | NC_000008.11 | 8 | 48607925 | - | LINC02847 | NNNGACTATCCTTCCTAACA | NGG | 2 | 0.0 | Tier III |
66 | NC_000023.11 | X | 149997506 | - | EOLA2-DT | NNNGACTATCCTTGCCAACA | NGG | 2 | 0.0 | Tier III |
67 | NC_000005.10 | 5 | 112925050 | + | LOC105379123 | NNNGACTATCCTTGCTAACA | NGG | 2 | 0.0 | Tier III |
68 | NC_000006.12 | 6 | 114507313 | - | LNCPOIR | NNNGACTATCCTTGCTAACA | NGG | 2 | 0.0 | Tier III |
69 | NC_000023.11 | X | 24093308 | + | LOC124905260 | NNNGACTATCCTTGCTAACA | NGG | 2 | 0.0 | Tier III |
70 | NC_000023.11 | X | 24093308 | + | LOC124905261 | NNNGACTATCCTTGCTAACA | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 90807993 | + | Col6a3 | NNNGACTATCCTCTCAATCA | NGG | 2 | 0.3947 | Tier I |
2 | NC_000075.6 | 9 | 108728205 | - | Prkar2a | NNNGACAATCTTTTCAAACA | NGG | 2 | 0.2692 | Tier I |
3 | NC_000069.6 | 3 | 68268995 | - | Schip1 | NNNAACTATTCTTTCAAACA | NGG | 2 | 0.8471 | Tier II |
4 | NC_000069.6 | 3 | 68268995 | - | Iqschfp | NNNAACTATTCTTTCAAACA | NGG | 2 | 0.8471 | Tier II |
5 | NC_000074.6 | 8 | 111386704 | - | Fa2h | NNNGGCTATCCTTTCAAACA | NGG | 1 | 0.72 | Tier II |
6 | NC_000086.7 | X | 93656775 | + | Pcyt1b | NNNAACTATCCTATCAAACA | NGG | 2 | 0.6231 | Tier II |
7 | NC_000079.6 | 13 | 63553977 | + | Ptch1 | NNNGGCTGTCCTTTCAAACA | NGG | 2 | 0.528 | Tier II |
8 | NC_000070.6 | 4 | 111821016 | - | Spata6 | NNNGACAATCCTTTCAAAAA | NGG | 2 | 0.375 | Tier II |
9 | NC_000068.7 | 2 | 50089284 | + | Lypd6 | NNNGACATTCCTTTCAAACA | NGG | 2 | 0.375 | Tier II |
10 | NC_000081.6 | 15 | 40686397 | - | Zfpm2 | NNNGACATTCCTTTCAAACA | NGG | 2 | 0.375 | Tier II |
11 | NC_000068.7 | 2 | 49141504 | + | Mbd5 | NNNGACTTTACTTTCAAACA | NGG | 2 | 0.3714 | Tier II |
12 | NC_000078.6 | 12 | 99298366 | + | Foxn3 | NNNGACTTTACTTTCAAACA | NGG | 2 | 0.3714 | Tier II |
13 | NC_000080.6 | 14 | 115400175 | + | Gpc5 | NNNGACTTTACTTTCAAACA | NGG | 2 | 0.3714 | Tier II |
14 | NC_000081.6 | 15 | 37577853 | + | Ncald | NNNGTTTATCCTTTCAAACA | NGG | 2 | 0.3377 | Tier II |
15 | NC_000067.6 | 1 | 84484857 | + | Dner | NNNGACTATCCATTCAAGCA | NGG | 2 | 0.32 | Tier II |
16 | NC_000069.6 | 3 | 127351040 | - | Ank2 | NNNGACTATTCTTTCAAACA | NAG | 2 | 0.244 | Tier II |
17 | NC_000067.6 | 1 | 135990411 | - | Igfn1 | NNNGACTATCTTTTCAATCA | NGG | 2 | 0.1538 | Tier II |
18 | NC_000067.6 | 1 | 124483100 | - | Dpp10 | NNNGACTATACTTTCACACA | NGG | 2 | 0.1529 | Tier II |
19 | NC_000077.6 | 11 | 48976129 | + | 9930111J21Rik1 | NNNGACTTTCCTTTAAAACA | NGG | 2 | 0.0952 | Tier II |
20 | NC_000077.6 | 11 | 49047970 | + | 9930111J21Rik2 | NNNGACTTTCCTTTAAAACA | NGG | 2 | 0.0952 | Tier II |
21 | NC_000075.6 | 9 | 15529994 | + | Smco4 | NNNGACTATACTTTCAAACA | NGA | 2 | 0.0602 | Tier II |
22 | NC_000068.7 | 2 | 12974888 | + | Pter | NNNGACTATCCTTTCACACA | NGA | 2 | 0.0123 | Tier II |
23 | NC_000070.6 | 4 | 66208882 | - | Astn2 | NNNGACTATCCTTTGAAACA | NGC | 2 | 0.0011 | Tier II |
24 | NC_000079.6 | 13 | 32196061 | + | Gmds | NNNGACTATCCTTTATAACA | NGG | 2 | 0.0 | Tier II |
25 | NC_000067.6 | 1 | 71622112 | - | Fn1 | NNNGAGTATCCTTTCCAACA | NGG | 2 | 0.0 | Tier II |
26 | NC_000083.6 | 17 | 60946865 | - | Gm33890 | NNNGACGATACTTTCAAACA | NGG | 2 | 0.5098 | Tier III |
27 | NC_000067.6 | 1 | 69434919 | - | Gm28497 | NNNGAATATCCTTTCAAATA | NGG | 2 | 0.4286 | Tier III |
28 | NC_000080.6 | 14 | 115400175 | + | 4930505G20Rik | NNNGACTTTACTTTCAAACA | NGG | 2 | 0.3714 | Tier III |
29 | NC_000068.7 | 2 | 75154403 | - | 2600014E21Rik | NNNGACTCTCCTTTCAAACT | NGG | 2 | 0.2571 | Tier III |
30 | NC_000076.6 | 10 | 25651965 | + | Gm29571 | NNNGACTTTCCTTTCAAACC | NGG | 2 | 0.0974 | Tier III |
31 | NC_000075.6 | 9 | 15529994 | + | Gm51703 | NNNGACTATACTTTCAAACA | NGA | 2 | 0.0602 | Tier III |