Construct: sgRNA BRDN0001145606
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGCTTACCAAGTAATCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5K1A (8394)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77961
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
151232358 |
- |
PIP5K1A |
NNNGCTTACCAAGTAATCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
68876787 |
- |
PIP5K1B |
NNNTCTTACCAAGTAATCAT |
NAG |
2 |
0.0943 |
Tier I |
| 3 |
NC_000019.10 |
19 |
3660974 |
+ |
PIP5K1C |
NNNGCTTACCAAGTAATCAT |
NTG |
1 |
0.039 |
Tier I |
| 4 |
NC_000016.10 |
16 |
71739404 |
+ |
AP1G1 |
NNNGCTTACCAAGAAATTAT |
NGG |
2 |
0.398 |
Tier II |
| 5 |
NC_000014.9 |
14 |
62710441 |
+ |
KCNH5 |
NNNGCTTCCCAAGTAAACAT |
NGG |
2 |
0.2286 |
Tier II |
| 6 |
NC_000012.12 |
12 |
42167959 |
- |
YAF2 |
NNNGCTTACCCAGTAATCTT |
NGG |
2 |
0.2154 |
Tier II |
| 7 |
NC_000003.12 |
3 |
76335980 |
+ |
ROBO2 |
NNNGCTTACCATGTAAACAT |
NGG |
2 |
0.1778 |
Tier II |
| 8 |
NC_000002.12 |
2 |
86713982 |
+ |
RNF103-CHMP3 |
NNNGCTTAACAAGTAATCAT |
NGA |
2 |
0.0595 |
Tier II |
| 9 |
NC_000023.11 |
X |
129566264 |
- |
OCRL |
NNNGCTTGCCAAGTAATCAT |
NGA |
2 |
0.0509 |
Tier II |
| 10 |
NC_000006.12 |
6 |
126203084 |
+ |
TRMT11 |
NNNGCTTACCAAGTAATCAT |
NGC |
1 |
0.0222 |
Tier II |
| 11 |
NC_000003.12 |
3 |
76335980 |
+ |
LOC124900544 |
NNNGCTTACCATGTAAACAT |
NGG |
2 |
0.1778 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
24390352 |
+ |
Pip5k1b |
NNNTCTTACCAAGTAATCAT |
NGG |
1 |
0.3636 |
Tier I |
| 2 |
NC_000069.6 |
3 |
95072411 |
+ |
Pip5k1a |
NNNACTTACCAAGTAATCAT |
NAG |
2 |
0.2333 |
Tier I |
| 3 |
NC_000068.7 |
2 |
38859676 |
- |
Nr6a1 |
NNNGCTTACCAAGTAACCAT |
NGG |
1 |
0.7059 |
Tier II |
| 4 |
NC_000086.7 |
X |
59276980 |
- |
Fgf13 |
NNNCCTTACCAACTAATCAT |
NGG |
2 |
0.2229 |
Tier II |
| 5 |
NC_000075.6 |
9 |
96202757 |
- |
Tfdp2 |
NNNGCTTACCATGTAAACAT |
NGG |
2 |
0.1778 |
Tier II |
| 6 |
NC_000083.6 |
17 |
50988630 |
- |
Tbc1d5 |
NNNGCTTACCAAGTAATAAT |
NGT |
2 |
0.0087 |
Tier II |
| 7 |
NC_000080.6 |
14 |
22576415 |
+ |
Lrmda |
NNNGCTTACCATGGAATCAT |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000068.7 |
2 |
38859676 |
- |
Nr6a1os |
NNNGCTTACCAAGTAACCAT |
NGG |
1 |
0.7059 |
Tier III |
| 9 |
NC_000068.7 |
2 |
132630068 |
- |
AU019990 |
NNNGCTTACCAAGTAGTCTT |
NGG |
2 |
0.1036 |
Tier III |
Other clones with same target sequence:
(none)
