Construct: sgRNA BRDN0001145615
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGAAGTTACCTACCAACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MYO3B (140469)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75570
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
170404398 |
- |
MYO3B |
NNNAAGTTACCTACCAACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
174498759 |
+ |
RABGAP1L |
NNNAAGTGACCTACCAACCT |
NGG |
2 |
0.44 |
Tier II |
| 3 |
NC_000015.10 |
15 |
41439553 |
+ |
RTF1 |
NNNAAATTACCTACCAACAA |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000001.11 |
1 |
243456277 |
- |
SDCCAG8 |
NNNAATTTCCCTACCAACCA |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000022.11 |
22 |
43322769 |
- |
SCUBE1 |
NNNAAGTCACCTACCAACCC |
NGG |
2 |
0.2273 |
Tier II |
| 6 |
NC_000020.11 |
20 |
36367273 |
- |
DLGAP4 |
NNNCAGTTACTTACCAACCA |
NGG |
2 |
0.1086 |
Tier II |
| 7 |
NC_000007.14 |
7 |
92779081 |
+ |
CDK6 |
NNNAAGTTACCTAACAACCC |
NGG |
2 |
0.0795 |
Tier II |
| 8 |
NC_000016.10 |
16 |
67228308 |
- |
TMEM208 |
NNNAAGTTACCTCCAAACCA |
NGG |
2 |
0.0468 |
Tier II |
| 9 |
NC_000008.11 |
8 |
30682459 |
+ |
GSR |
NNNAAGTTACCTACCAACTA |
NGT |
2 |
0.0074 |
Tier II |
| 10 |
NC_000002.12 |
2 |
29104801 |
- |
CLIP4 |
NNNAAGTGACCTACCTACCA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000004.12 |
4 |
117449353 |
+ |
LINC01378 |
NNNGAGTAACCTACCAACCA |
NGG |
2 |
0.5 |
Tier III |
| 12 |
NC_000008.11 |
8 |
11261610 |
- |
LINC00529 |
NNNAACTTACCTACCGACCA |
NGG |
2 |
0.1311 |
Tier III |
| 13 |
NC_000002.12 |
2 |
29104801 |
- |
LOC105374386 |
NNNAAGTGACCTACCTACCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
70255398 |
- |
Myo3b |
NNNAAGGTACGTACCAACCA |
NGG |
2 |
0.1471 |
Tier I |
| 2 |
NC_000072.6 |
6 |
34800231 |
+ |
Agbl3 |
NNNAAATTAACTACCAACCA |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000078.6 |
12 |
38032791 |
- |
Dgkb |
NNNAAGTTAACTACCAACCA |
NGG |
1 |
0.8667 |
Tier II |
| 4 |
NC_000079.6 |
13 |
100704619 |
- |
Cdk7 |
NNNAAGTTACAAACCAACCA |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000070.6 |
4 |
86373322 |
+ |
Adamtsl1 |
NNNAATTTACATACCAACCA |
NGG |
2 |
0.5 |
Tier II |
| 6 |
NC_000071.6 |
5 |
107646164 |
- |
Rpap2 |
NNNAAGTTCCATACCAACCA |
NGG |
2 |
0.4286 |
Tier II |
| 7 |
NC_000084.6 |
18 |
65437736 |
+ |
Malt1 |
NNNTAGTTTCCTACCAACCA |
NGG |
2 |
0.3818 |
Tier II |
| 8 |
NC_000080.6 |
14 |
117929071 |
+ |
Gpc6 |
NNNAAGTTACAGACCAACCA |
NGG |
2 |
0.375 |
Tier II |
| 9 |
NC_000077.6 |
11 |
6136504 |
+ |
Nudcd3 |
NNNAAGTGACCGACCAACCA |
NGG |
2 |
0.3667 |
Tier II |
| 10 |
NC_000071.6 |
5 |
48222358 |
+ |
Slit2 |
NNNAAGTTACCTACTAATCA |
NGG |
2 |
0.0429 |
Tier II |
| 11 |
NC_000085.6 |
19 |
38885756 |
- |
Tbc1d12 |
NNNAAGTTACCTACCAAGCC |
NGG |
2 |
0.0303 |
Tier II |
| 12 |
NC_000080.6 |
14 |
62686887 |
+ |
Ints6 |
NNNAAGCTACCTACCAACCA |
NTG |
2 |
0.0268 |
Tier II |
| 13 |
NC_000080.6 |
14 |
62686887 |
+ |
Serpine3 |
NNNAAGCTACCTACCAACCA |
NTG |
2 |
0.0268 |
Tier II |
| 14 |
NC_000071.6 |
5 |
44977413 |
+ |
4930431F12Rik |
NNNAAGTTACCTACCTAGCA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
