Construct: sgRNA BRDN0001145640
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCATGGAGTACTGCCAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IKBKB (3551)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76088
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000008.11 |
8 |
42290255 |
+ |
IKBKB |
NNNATGGAGTACTGCCAAGG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000004.12 |
4 |
90387714 |
- |
CCSER1 |
NNNATGGAGTACTGCCAAAA |
NGG |
2 |
0.6696 |
Tier II |
3 |
NC_000005.10 |
5 |
65788025 |
+ |
NLN |
NNNATTGAGTACTGCAAAGG |
NGG |
2 |
0.6667 |
Tier II |
4 |
NC_000019.10 |
19 |
50426445 |
+ |
SPIB |
NNNATGTAGAACTGCCAAGG |
NGG |
2 |
0.4898 |
Tier II |
5 |
NC_000014.9 |
14 |
85532873 |
- |
FLRT2 |
NNNATGGTGTACTGCCAAGA |
NGG |
2 |
0.4018 |
Tier II |
6 |
NC_000023.11 |
X |
150444673 |
+ |
MAMLD1 |
NNNATGCTGTACTGCCAAGG |
NGG |
2 |
0.2946 |
Tier II |
7 |
NC_000002.12 |
2 |
221479932 |
- |
EPHA4 |
NNNATGTAGTCCTGCCAAGG |
NGG |
2 |
0.2286 |
Tier II |
8 |
NC_000001.11 |
1 |
7693754 |
+ |
CAMTA1 |
NNNATGGAGTACTGCCAACC |
NGG |
2 |
0.1921 |
Tier II |
9 |
NC_000001.11 |
1 |
165546009 |
+ |
LRRC52 |
NNNATGAAGTACTGCCCAGG |
NGG |
2 |
0.1765 |
Tier II |
10 |
NC_000002.12 |
2 |
63897792 |
- |
VPS54 |
NNNAAGGAGTACTGCTAAGG |
NGG |
2 |
0.1538 |
Tier II |
11 |
NC_000003.12 |
3 |
9185151 |
- |
SRGAP3 |
NNNATGGAATACTGCCAAGG |
NGA |
2 |
0.0446 |
Tier II |
12 |
NC_000010.11 |
10 |
62388292 |
+ |
ZNF365 |
NNNATGGAGTACTGCCTAGG |
NTG |
2 |
0.0052 |
Tier II |
13 |
NC_000008.11 |
8 |
52767931 |
- |
LOC105375835 |
NNNATGGAGTACAGCCAGGG |
NGG |
2 |
0.2769 |
Tier III |
14 |
NC_000001.11 |
1 |
7693754 |
+ |
LOC105376689 |
NNNATGGAGTACTGCCAACC |
NGG |
2 |
0.1921 |
Tier III |
15 |
NC_000001.11 |
1 |
165546009 |
+ |
LRRC52-AS1 |
NNNATGAAGTACTGCCCAGG |
NGG |
2 |
0.1765 |
Tier III |
16 |
NC_000012.12 |
12 |
30253500 |
- |
LINC02386 |
NNNATGGAGTACTGCCAATG |
NAG |
2 |
0.1728 |
Tier III |
17 |
NC_000001.11 |
1 |
58894255 |
- |
JUN-DT |
NNNATGGAGTACTGGCAAGG |
NAG |
2 |
0.013 |
Tier III |
18 |
NC_000001.11 |
1 |
58894255 |
- |
LINC02777 |
NNNATGGAGTACTGGCAAGG |
NAG |
2 |
0.013 |
Tier III |
19 |
NC_000002.12 |
2 |
96226062 |
- |
STARD7-AS1 |
NNNATGGAGTACTGGCAAGG |
NTG |
2 |
0.0019 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000074.6 |
8 |
22693834 |
- |
Ikbkb |
NNNATGGAGTACTGCCAAGG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000068.7 |
2 |
129810552 |
+ |
Stk35 |
NNNATGGAGTACTGTGAAGG |
NGG |
2 |
0.0103 |
Tier I |
3 |
NC_000078.6 |
12 |
81032442 |
+ |
Smoc1 |
NNNATGGAGAACTGCCATGG |
NGG |
2 |
0.4286 |
Tier II |
4 |
NC_000068.7 |
2 |
127647949 |
+ |
Mal |
NNNATGGGTTACTGCCAAGG |
NGG |
2 |
0.3911 |
Tier II |
5 |
NC_000073.6 |
7 |
6725564 |
- |
Peg3 |
NNNTTGGAGTATTGCCAAGG |
NGG |
2 |
0.3427 |
Tier II |
6 |
NC_000070.6 |
4 |
152220869 |
+ |
Acot7 |
NNNAGGGAGCACTGCCAAGG |
NGG |
2 |
0.3413 |
Tier II |
7 |
NC_000070.6 |
4 |
152220892 |
+ |
Acot7 |
NNNAGGGAGCACTGCCAAGG |
NGG |
2 |
0.3413 |
Tier II |
8 |
NC_000086.7 |
X |
57095771 |
+ |
Vgll1 |
NNNCTTGAGTACTGCCAAGG |
NGG |
2 |
0.2353 |
Tier II |
9 |
NC_000068.7 |
2 |
113410106 |
+ |
Fmn1 |
NNNATGGAGTGCTGCCAAGG |
NAG |
2 |
0.1685 |
Tier II |
10 |
NC_000078.6 |
12 |
75092102 |
- |
Kcnh5 |
NNNATGGAGTCCTGCCAAGG |
NAG |
2 |
0.1037 |
Tier II |
11 |
NC_000068.7 |
2 |
103581620 |
- |
Abtb2 |
NNNATGGAGTACTGGAAAGG |
NGG |
2 |
0.05 |
Tier II |
12 |
NC_000081.6 |
15 |
75781972 |
+ |
Zc3h3 |
NNNATGGAGTACTGTCCAGG |
NGG |
2 |
0.0118 |
Tier II |
13 |
NC_000076.6 |
10 |
43891211 |
- |
Qrsl1 |
NNNATGGAGTACTGACAAGG |
NTG |
2 |
0.0087 |
Tier II |
14 |
NC_000068.7 |
2 |
114973865 |
- |
Gm13974 |
NNNATGAGGTACTGCCAAGG |
NGG |
2 |
0.7333 |
Tier III |
15 |
NC_000068.7 |
2 |
103581620 |
- |
8030431J09Rik |
NNNATGGAGTACTGGAAAGG |
NGG |
2 |
0.05 |
Tier III |
16 |
NC_000070.6 |
4 |
89354125 |
+ |
Gm12610 |
NNNATGGAGTACTGCGTAGG |
NGG |
2 |
0.0205 |
Tier III |
Other clones with same target sequence:
(none)