Construct: sgRNA BRDN0001145649
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAAGGTGCAATGTGCGAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TEC (7006)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77025
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
48145442 |
- |
TEC |
NNNAGGTGCAATGTGCGAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
26775095 |
- |
ARID1A |
NNNAGGTGCTATGTGCGAGG |
NAG |
2 |
0.2288 |
Tier I |
| 3 |
NC_000017.11 |
17 |
79515658 |
- |
RBFOX3 |
NNNAGGGGCAATGTGGGAGG |
NGG |
2 |
0.0905 |
Tier II |
| 4 |
NC_000023.11 |
X |
119117678 |
- |
KIAA1210 |
NNNAGGTGCAAGGTGCGAGG |
NTG |
2 |
0.0195 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
100293542 |
- |
Ror1 |
NNNAGGTGCAATGTGCAATG |
NGG |
2 |
0.6222 |
Tier II |
| 2 |
NC_000079.6 |
13 |
98156623 |
+ |
Arhgef28 |
NNNAGGGGAAATGTGCGAGG |
NGG |
2 |
0.5042 |
Tier II |
| 3 |
NC_000075.6 |
9 |
115951623 |
- |
Gadl1 |
NNNAGGTGCAAGGTGCAAGG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000076.6 |
10 |
27345879 |
- |
Lama2 |
NNNAGTTGCCATGTGCGAGG |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000081.6 |
15 |
55519811 |
- |
Col14a1 |
NNNAGGTGCAATGTGTGAGG |
NGA |
2 |
0.0214 |
Tier II |
| 6 |
NC_000075.6 |
9 |
115951623 |
- |
Gm31234 |
NNNAGGTGCAAGGTGCAAGG |
NGG |
2 |
0.4667 |
Tier III |
Other clones with same target sequence:
(none)
