Construct: sgRNA BRDN0001145658
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTATAGGCCATTGGGTACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKA (1606)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76607
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
55937419 |
+ |
DGKA |
NNNTAGGCCATTGGGTACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
11823713 |
+ |
CTNND2 |
NNNTAGGCCATTAGGTACCA |
NGG |
2 |
0.4138 |
Tier II |
| 3 |
NC_000007.14 |
7 |
132344052 |
- |
PLXNA4 |
NNNTTGGCCATTGGGTACTA |
NGG |
2 |
0.2424 |
Tier II |
| 4 |
NC_000010.11 |
10 |
97582154 |
+ |
ANKRD2 |
NNNTAGGCCATTGGATAGGA |
NGG |
2 |
0.1255 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
29456003 |
+ |
Ccdc88a |
NNNTAGGCCAGTGGGTAAGA |
NGG |
2 |
0.2154 |
Tier II |
| 2 |
NC_000080.6 |
14 |
38635749 |
- |
Nrg3 |
NNNTAGGCCATTGGCTATGA |
NGG |
2 |
0.1753 |
Tier II |
| 3 |
NC_000071.6 |
5 |
73079151 |
- |
Fryl |
NNNTAGGCCAGTGGCTACGA |
NGG |
2 |
0.1091 |
Tier II |
| 4 |
NC_000074.6 |
8 |
127236474 |
+ |
Pard3 |
NNNTAGGCCATTGTGTACGA |
NGA |
2 |
0.0185 |
Tier II |
| 5 |
NC_000071.6 |
5 |
131407215 |
- |
Gm29681 |
NNNTAGGACATTGGGTAAGA |
NGG |
2 |
0.35 |
Tier III |
| 6 |
NC_000071.6 |
5 |
4311859 |
- |
Gm40261 |
NNNTAGGCCACTGGGTACCA |
NGG |
2 |
0.2989 |
Tier III |
Other clones with same target sequence:
(none)
