Construct: sgRNA BRDN0001145729
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCGGGTACTTGGTAAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KB1 (6198)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75612
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
59912789 |
+ |
RPS6KB1 |
NNNCGGGTACTTGGTAAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
123621483 |
- |
THOC2 |
NNNAGGGTACTTGGTAAAGA |
NGG |
2 |
0.7895 |
Tier I |
| 3 |
NC_000015.10 |
15 |
86875956 |
+ |
AGBL1 |
NNNCAGGTACTTGGTAAAGA |
NGG |
2 |
0.8125 |
Tier II |
| 4 |
NC_000016.10 |
16 |
23399407 |
- |
COG7 |
NNNTGGGTACTTGGTAAATG |
NGG |
2 |
0.5333 |
Tier II |
| 5 |
NC_000010.11 |
10 |
30910177 |
+ |
ZNF438 |
NNNTGTGTACTTGGTAAAGG |
NGG |
2 |
0.5333 |
Tier II |
| 6 |
NC_000015.10 |
15 |
82540448 |
+ |
RPS17 |
NNNCGGGTCCTTGGTAAAAG |
NGG |
2 |
0.4082 |
Tier II |
| 7 |
NC_000001.11 |
1 |
25301180 |
- |
RHD |
NNNGGGGAACTTGGTAAAGG |
NGG |
2 |
0.4 |
Tier II |
| 8 |
NC_000001.11 |
1 |
25301180 |
- |
RSRP1 |
NNNGGGGAACTTGGTAAAGG |
NGG |
2 |
0.4 |
Tier II |
| 9 |
NC_000014.9 |
14 |
32587965 |
+ |
AKAP6 |
NNNGGGGTACATGGTAAAGG |
NGG |
2 |
0.375 |
Tier II |
| 10 |
NC_000020.11 |
20 |
59982778 |
+ |
CDH26 |
NNNGGGGTACTTGGTAAATG |
NGG |
2 |
0.3333 |
Tier II |
| 11 |
NC_000004.12 |
4 |
84695054 |
+ |
WDFY3 |
NNNCTGGTACTTAGTAAAGG |
NGG |
2 |
0.2769 |
Tier II |
| 12 |
NC_000006.12 |
6 |
52069444 |
+ |
PKHD1 |
NNNGGGGTACTTGGTGAAGG |
NGG |
2 |
0.0962 |
Tier II |
| 13 |
NC_000001.11 |
1 |
205564066 |
+ |
MFSD4A-AS1 |
NNNCGGATATTTGGTAAAGG |
NGG |
2 |
0.9412 |
Tier III |
| 14 |
NC_000010.11 |
10 |
57729264 |
- |
LOC105378314 |
NNNTGGGTATTTGGTAAAGG |
NGG |
2 |
0.7529 |
Tier III |
| 15 |
NC_000008.11 |
8 |
124198344 |
- |
LOC101927588 |
NNNCTGGTACTTGGTAAAGG |
NGC |
2 |
0.0067 |
Tier III |
| 16 |
NC_000006.12 |
6 |
10475642 |
+ |
LINC02522 |
NNNGGGGTACTTGGTCAAGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
86534042 |
- |
Rps6kb1 |
NNNCGGGTACTTGGTAAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000083.6 |
17 |
70849636 |
- |
Tgif1 |
NNNCGGGTACTTGGTTAAGT |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000072.6 |
6 |
56358080 |
- |
Pde1c |
NNNCAGATACTTGGTAAAGG |
NGG |
2 |
0.8667 |
Tier II |
| 4 |
NC_000081.6 |
15 |
12908679 |
+ |
Drosha |
NNNTGGGTACTTAGTAAAGG |
NGG |
2 |
0.7385 |
Tier II |
| 5 |
NC_000078.6 |
12 |
22714158 |
- |
Gm38538 |
NNNTGCGTACTTGGTAAAGG |
NGG |
2 |
0.5455 |
Tier II |
| 6 |
NC_000078.6 |
12 |
24060069 |
- |
9030624G23Rik |
NNNTGCGTACTTGGTAAAGG |
NGG |
2 |
0.5455 |
Tier II |
| 7 |
NC_000079.6 |
13 |
49051715 |
- |
Wnk2 |
NNNCTGGGACTTGGTAAAGG |
NGG |
2 |
0.22 |
Tier II |
| 8 |
NC_000078.6 |
12 |
99275750 |
- |
Foxn3 |
NNNCGGGTACTTGGTAAAGC |
NTG |
2 |
0.0167 |
Tier II |
Other clones with same target sequence:
(none)
