Construct: sgRNA BRDN0001145758
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTACAACGGGCACACGAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LCK (3932)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76413
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
32276628 |
+ |
LCK |
NNNCAACGGGCACACGAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
403214 |
+ |
PKP3 |
NNNCAACCGGCACACGACGG |
NGG |
2 |
0.1172 |
Tier I |
| 3 |
NC_000019.10 |
19 |
35555706 |
+ |
ATP4A |
NNNCTACGGGCACACGGAGG |
NGG |
2 |
0.0642 |
Tier I |
| 4 |
NC_000007.14 |
7 |
100690918 |
+ |
GIGYF1 |
NNNCAAGGGGCACACAAAGG |
NGG |
2 |
0.4706 |
Tier II |
| 5 |
NC_000016.10 |
16 |
61091 |
+ |
RHBDF1 |
NNNCACCGGGCAGACGAAGG |
NGG |
2 |
0.062 |
Tier II |
| 6 |
NC_000015.10 |
15 |
32286009 |
- |
LOC102724078 |
NNNCTACGGGCACACGCAGG |
NGG |
2 |
0.0642 |
Tier III |
| 7 |
NC_000013.11 |
13 |
31880627 |
- |
EEF1DP3 |
NNNCTACGGGCACAGGAAGG |
NGG |
2 |
0.0182 |
Tier III |
| 8 |
NC_000017.11 |
17 |
15816032 |
+ |
LINC02087 |
NNNCAACGGGCACAGGAAGG |
NTG |
2 |
0.0019 |
Tier III |
| 9 |
NC_000017.11 |
17 |
20631539 |
+ |
LINC02088 |
NNNCAACGGGCACAGGAAGG |
NTG |
2 |
0.0019 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
129555698 |
- |
Lck |
NNNCAACGGACACACGAAGG |
NGG |
1 |
0.9333 |
Tier I |
Other clones with same target sequence:
(none)
