Construct: sgRNA BRDN0001145770

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCAGAAACATATCTAAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO3A (53904)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76224

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 26143511 - MYO3A NNNAGAAACATATCTAAGAG NGG 0 1.0 Tier I
2 NC_000005.10 5 57237169 - GPBP1 NNNGGAAACATACCTAAGAG NGG 2 0.4934 Tier I
3 NC_000004.12 4 68120916 - TMPRSS11F NNNAAAAACATATCTAAGAG NGG 1 0.8667 Tier II
4 NC_000008.11 8 116943166 + AARD NNNAGAAAAACATCTAAGAG NGG 2 0.5714 Tier II
5 NC_000007.14 7 41000980 + SUGCT NNNAGAAACATGTTTAAGAG NGG 2 0.5296 Tier II
6 NC_000012.12 12 86721356 + MGAT4C NNNAGAAACATAATTAAGAG NGG 2 0.5077 Tier II
7 NC_000001.11 1 213177802 - RPS6KC1 NNNAGAAATAGATCTAAGAG NGG 2 0.35 Tier II
8 NC_000023.11 X 77561556 + ATRX NNNAGGTACATATCTAAGAG NGG 2 0.3125 Tier II
9 NC_000002.12 2 44297030 - SLC3A1 NNNAGAGACATATCTAAGAC NGG 2 0.3025 Tier II
10 NC_000018.10 18 32722210 - KLHL14 NNNAGAGACATATCTAAGAC NGG 2 0.3025 Tier II
11 NC_000011.10 11 12939303 - TEAD1 NNNAGAAACATCCCTAAGAG NGG 2 0.2078 Tier II
12 NC_000002.12 2 25426745 - DTNB NNNATAAAGATATCTAAGAG NGG 2 0.1857 Tier II
13 NC_000002.12 2 159888208 + LY75 NNNATAAAGATATCTAAGAG NGG 2 0.1857 Tier II
14 NC_000002.12 2 159888208 + LY75-CD302 NNNATAAAGATATCTAAGAG NGG 2 0.1857 Tier II
15 NC_000009.12 9 109912958 - PALM2AKAP2 NNNAGAAACATATCTAAGCT NGG 2 0.1448 Tier II
16 NC_000005.10 5 133605764 - FSTL4 NNNAGAAACAGATATAAGAG NGG 2 0.14 Tier II
17 NC_000003.12 3 111681765 - PLCXD2 NNNAGAAACATGTCTGAGAG NGG 2 0.1389 Tier II
18 NC_000011.10 11 579342 - PHRF1 NNNAGAAACATATCTGAAAG NGG 2 0.1331 Tier II
19 NC_000008.11 8 139734533 + TRAPPC9 NNNATAAACATTTCTAAGAG NGG 2 0.1 Tier II
20 NC_000006.12 6 44819516 + SUPT3H NNNAGAAACATAGATAAGAG NGG 2 0.0913 Tier II
21 NC_000017.11 17 31282785 + NF1 NNNAGATACATATCTAGGAG NGG 2 0.0772 Tier II
22 NC_000009.12 9 121664054 - DAB2IP NNNAGAAACAGATCTAAGAG NGA 2 0.0278 Tier II
23 NC_000022.11 22 38149296 + PLA2G6 NNNAGAAACATATCTGTGAG NGG 2 0.0256 Tier II
24 NC_000001.11 1 237616311 - RYR2 NNNAGAAACATTTCTAAGAG NGA 2 0.0231 Tier II
25 NC_000009.12 9 95466019 + PTCH1 NNNAGAAACATTTCTAAGAG NGA 2 0.0231 Tier II
26 NC_000010.11 10 68430756 + DNA2 NNNAGAACCATATCTAAGAG NTG 2 0.0167 Tier II
27 NC_000021.9 21 39259247 - BRWD1 NNNAAAAACATATCTAAGAG NGT 2 0.014 Tier II
28 NC_000003.12 3 19311593 + KCNH8 NNNAGAAACATATCTGAGAG NGA 2 0.0134 Tier II
29 NC_000004.12 4 154752804 - LRAT NNNAGAAACATATCTAACAG NGC 2 0.0106 Tier II
30 NC_000016.10 16 31370894 - ITGAX NNNAGAAACAGATGTAAGAG NGG 2 0.0 Tier II
31 NC_000015.10 15 86805561 + AGBL1 NNNAGAAACATATCTCAGAG NGT 2 0.0 Tier II
32 NC_000009.12 9 29064578 - LINGO2 NNNAGAAACATATGTAAAAG NGG 2 0.0 Tier II
33 NC_000013.11 13 43458990 - ENOX1 NNNAGAAACATATGTTAGAG NGG 2 0.0 Tier II
34 NC_000003.12 3 64789883 - ADAMTS9-AS2 NNNAGAAACTCATCTAAGAG NGG 2 0.5882 Tier III
35 NC_000002.12 2 221676240 + LOC105373899 NNNAGAAACATGTCTAATAG NGG 2 0.4815 Tier III
36 NC_000024.10 Y 3005780 + LINC00278 NNNAGAAACACATCTAAGAT NGG 2 0.4667 Tier III
37 NC_000005.10 5 114758496 + LOC101927078 NNNAGAAACATATCTAAGAC NGG 1 0.4286 Tier III
38 NC_000011.10 11 122198328 + MIR100HG NNNAGAAACATAACAAAGAG NGG 2 0.4008 Tier III
39 NC_000006.12 6 135644155 + AHI1-DT NNNAGAAACATATTTAAGAC NGG 2 0.3143 Tier III
40 NC_000018.10 18 32722210 - LOC112268208 NNNAGAGACATATCTAAGAC NGG 2 0.3025 Tier III
41 NC_000021.9 21 35455284 + LOC100506403 NNNATAAACAAATCTAAGAG NGG 2 0.225 Tier III
42 NC_000010.11 10 8580347 - LOC105376398 NNNAGAAACATATTTAAGAG NAG 2 0.1901 Tier III
43 NC_000002.12 2 25426745 - DTNB-AS1 NNNATAAAGATATCTAAGAG NGG 2 0.1857 Tier III
44 NC_000006.12 6 44819516 + LOC101929770 NNNAGAAACATAGATAAGAG NGG 2 0.0913 Tier III
45 NC_000009.12 9 95466019 + LOC100507346 NNNAGAAACATTTCTAAGAG NGA 2 0.0231 Tier III
46 NC_000006.12 6 74271615 - LOC101928516 NNNAGAGACATATCTAAGAG NGC 2 0.0157 Tier III
47 NC_000004.12 4 129137311 - ZSWIM5P3 NNNAGAGACATATCTAAGAG NGT 2 0.0114 Tier III
48 NC_000011.10 11 72164311 - FOLR1-AS1 NNNAGAAACATAGGTAAGAG NGG 2 0.0 Tier III
49 NC_000013.11 13 43458990 - ENOX1-AS2 NNNAGAAACATATGTTAGAG NGG 2 0.0 Tier III
50 NC_000007.14 7 7071338 - LOC105375138 NNNTGAAACATATCTCAGAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 111447900 + Gpbp1 NNNGGAAACATACCTAAGAG NGG 2 0.4934 Tier I
2 NC_000068.7 2 22423280 - Myo3a NNNAGAAACATGTCCAAGAG NGG 2 0.197 Tier I
3 NC_000068.7 2 42281198 + Lrp1b NNNAGAAACATATCTAATAA NGG 2 0.625 Tier II
4 NC_000077.6 11 35620210 + Slit3 NNNAGAAAGATATCTAAGAG NGG 1 0.619 Tier II
5 NC_000077.6 11 63136129 + Pmp22 NNNAGAAGCATATCTAAAAG NGG 2 0.5077 Tier II
6 NC_000085.6 19 17102336 + Prune2 NNNAAAAACATATCTAAGTG NGG 2 0.4667 Tier II
7 NC_000082.6 16 91734240 - Itsn1 NNNAGAAACACATCTAAAAG NGG 2 0.4615 Tier II
8 NC_000068.7 2 143800609 + Pcsk2 NNNAGACACATATCTAAGAA NGG 2 0.4102 Tier II
9 NC_000078.6 12 37974652 + Dgkb NNNAAAAACATATATAAGAG NGG 2 0.3033 Tier II
10 NC_000070.6 4 125562509 - Grik3 NNNCCAAACATATCTAAGAG NGG 2 0.2773 Tier II
11 NC_000085.6 19 24722403 + Pgm5 NNNAGAAAGAGATCTAAGAG NGG 2 0.2476 Tier II
12 NC_000072.6 6 15135640 - Foxp2 NNNAGAAACATAAATAAGAG NGG 2 0.2423 Tier II
13 NC_000082.6 16 6172606 + Rbfox1 NNNAGAAACACATATAAGAG NGG 2 0.2333 Tier II
14 NC_000071.6 5 24956098 - Prkag2 NNNAGAAACATAGCTAAGAC NGG 2 0.1118 Tier II
15 NC_000076.6 10 119501189 + Grip1 NNNACAAACATATCTATGAG NGG 2 0.1048 Tier II
16 NC_000081.6 15 10971510 - C1qtnf3 NNNAGAGACATATCTATGAG NGG 2 0.0941 Tier II
17 NC_000068.7 2 145435074 + Slc24a3 NNNAGAAACTTATCTAAGAG NGA 2 0.0613 Tier II
18 NC_000071.6 5 124421352 + Sbno1 NNNATAAACATATCTAGGAG NGG 2 0.0529 Tier II
19 NC_000074.6 8 75734737 - Iqcm NNNAGAAACATATCTGAGAG NAG 2 0.0499 Tier II
20 NC_000068.7 2 14118571 + Stam NNNAGAAAGATATCTAAGAG NGA 2 0.043 Tier II
21 NC_000068.7 2 175277098 - Gm14440 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
22 NC_000068.7 2 175374472 - Zfp968 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
23 NC_000068.7 2 175472023 - Gm4724 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
24 NC_000068.7 2 175472023 - Zfp965 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
25 NC_000068.7 2 175604140 + Gm4724 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
26 NC_000068.7 2 175701689 + Gm4724 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
27 NC_000068.7 2 175799065 + Gm14288 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
28 NC_000068.7 2 175929091 + Zfp966 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
29 NC_000068.7 2 176523014 - Zfp967 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
30 NC_000068.7 2 176672560 - Zfp973 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
31 NC_000068.7 2 177466745 - Zfp970 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
32 NC_000068.7 2 178025282 - Zfp971 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier II
33 NC_000079.6 13 93165210 + Tent2 NNNAAAAACATATCTAAGAG NTG 2 0.0338 Tier II
34 NC_000076.6 10 90293071 + Anks1b NNNAGAAACATATCTAAAAG NTG 2 0.027 Tier II
35 NC_000086.7 X 160916147 + Cdkl5 NNNAGAAACATATAGAAGAG NGG 2 0.0175 Tier II
36 NC_000075.6 9 83841752 - Ttk NNNAGAAACAGATCTAAGAG NTG 2 0.0156 Tier II
37 NC_000076.6 10 9267252 + Samd5 NNNAGAAACATATCTAGGAG NGA 2 0.0123 Tier II
38 NC_000078.6 12 100657361 - Rps6ka5 NNNAGAAACAGATCTAAGAG NGC 2 0.0089 Tier II
39 NC_000077.6 11 89423964 - Ankfn1 NNNAGAAACATTTCTAAGAG NGC 2 0.0074 Tier II
40 NC_000070.6 4 84923283 - Cntln NNNAAAAACATATGTAAGAG NGG 2 0.0 Tier II
41 NC_000081.6 15 50721188 + Trps1 NNNAGAAACATATCCTAGAG NGG 2 0.0 Tier II
42 NC_000077.6 11 60043852 - Pemt NNNAGAAACATATGAAAGAG NGG 2 0.0 Tier II
43 NC_000086.7 X 61012608 - Gm14664 NNNAGAAACTTATCTAAGAG NAG 2 0.2288 Tier III
44 NC_000079.6 13 46480476 - Gm35110 NNNAGAAACATATATAACAG NGG 2 0.1667 Tier III
45 NC_000078.6 12 114667686 + Igh NNNAGAAACATATCTAACAG NAG 2 0.1235 Tier III
46 NC_000078.6 12 114774750 + Igh NNNAGAAACATATCTAACAG NAG 2 0.1235 Tier III
47 NC_000078.6 12 114774750 + Gm40899 NNNAGAAACATATCTAACAG NAG 2 0.1235 Tier III
48 NC_000081.6 15 10971510 - Gm34315 NNNAGAGACATATCTATGAG NGG 2 0.0941 Tier III
49 NC_000068.7 2 176425629 - Zfp968-ps NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier III
50 NC_000068.7 2 177899151 - Gm14327 NNNAGAAACATCTCTATGAG NGG 2 0.0351 Tier III
51 NC_000067.6 1 161568387 + Gm31815 NNNAGAAACACATCTAAGAG NTG 2 0.026 Tier III
52 NC_000071.6 5 108896134 - Gm42627 NNNAGAAACATATGTAACAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)