Construct: sgRNA BRDN0001145785

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCGAGCCAATATGAACACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STYK1 (55359)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75695

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 10631093 - STYK1 NNNAGCCAATATGAACACTG NGG 0 1.0 Tier I
2 NC_000003.12 3 66262219 + SLC25A26 NNNAGCTAATACGAACACTG NGG 2 0.7697 Tier II
3 NC_000013.11 13 46728808 - LRCH1 NNNAGTTAATATGAACACTG NGG 2 0.7545 Tier II
4 NC_000003.12 3 158526667 - RSRC1 NNNAGCCAATAAAAACACTG NGG 2 0.7385 Tier II
5 NC_000023.11 X 148826616 - AFF2 NNNACTCAATATGAACACTG NGG 2 0.7296 Tier II
6 NC_000004.12 4 89275024 + GPRIN3 NNNACCAAATATGAACACTG NGG 2 0.5893 Tier II
7 NC_000003.12 3 94012611 - ARL13B NNNTACCAATATGAACACTG NGG 2 0.5515 Tier II
8 NC_000024.10 Y 6942609 - TBL1Y NNNAGCCAACATGAACACTA NGG 2 0.5 Tier II
9 NC_000006.12 6 35952437 + SLC26A8 NNNAGCCAATATATACACTG NGG 2 0.4923 Tier II
10 NC_000004.12 4 100410819 + EMCN NNNGGCCAATATGAACACTT NGG 2 0.4375 Tier II
11 NC_000006.12 6 97213549 - MMS22L NNNAGCCACTATGAACACTT NGG 2 0.4 Tier II
12 NC_000023.11 X 17745962 - SCML1 NNNAGCCAAACTGAACACTG NGG 2 0.3429 Tier II
13 NC_000010.11 10 98140124 - R3HCC1L NNNAGCCAATATGAACACTA NAG 2 0.2431 Tier II
14 NC_000005.10 5 179118373 + ADAMTS2 NNNCGCCAAGATGAACACTG NGG 2 0.1765 Tier II
15 NC_000006.12 6 69683271 + LMBRD1 NNNAGCCAATAAGATCACTG NGG 2 0.16 Tier II
16 NC_000007.14 7 116681810 + MET NNNAGCCAATATGATCACTT NGG 2 0.14 Tier II
17 NC_000017.11 17 79786958 - CBX2 NNNAGCCAATATGGCCACTG NGG 2 0.1273 Tier II
18 NC_000002.12 2 1423798 - TPO NNNAGCCAATATGAACAGTA NGG 2 0.125 Tier II
19 NC_000003.12 3 5155528 - ARL8B NNNAGCCAACATGCACACTG NGG 2 0.1143 Tier II
20 NC_000004.12 4 109516905 + SEC24B NNNAGCCAATCTGAACACAG NGG 2 0.1143 Tier II
21 NC_000012.12 12 120771225 - SPPL3 NNNAGCCAGTATGAAGACTG NGG 2 0.1026 Tier II
22 NC_000003.12 3 130541258 - COL6A6 NNNAGCCAATCTGATCACTG NGG 2 0.08 Tier II
23 NC_000008.11 8 100278570 + RNF19A NNNAGCTAATATGAACACTG NGA 2 0.0564 Tier II
24 NC_000023.11 X 67713306 + AR NNNAGCCAATAAGAACACTG NGA 2 0.0556 Tier II
25 NC_000003.12 3 62765201 + CADPS NNNAGCCAATATGCACACTG NGA 2 0.0149 Tier II
26 NC_000012.12 12 121979021 - CFAP251 NNNAGCCAACATGAACACTG NGT 2 0.0086 Tier II
27 NC_000006.12 6 18572024 + MIR548A1HG NNNAGCTAACATGAACACTG NGG 2 0.4333 Tier III
28 NC_000008.11 8 63295667 - LOC105375873 NNNAGCCACTATGAACAATG NGG 2 0.3077 Tier III
29 NC_000012.12 12 59522124 + LINC02448 NNNAGCCAATATGAAAACTG NAG 2 0.2593 Tier III
30 NC_000008.11 8 29932122 - LINC02209 NNNACCCAATATGATCACTG NGG 2 0.1571 Tier III
31 NC_000007.14 7 63927806 - LOC105375318 NNNAGCCAAGTTGAACACTG NGG 2 0.1538 Tier III
32 NC_000007.14 7 63927806 - LOC124901653 NNNAGCCAAGTTGAACACTG NGG 2 0.1538 Tier III
33 NC_000019.10 19 27795551 - LINC02987 NNNAGCCAAGTTGAACACTG NGG 2 0.1538 Tier III
34 NC_000007.14 7 116681810 + COMETT NNNAGCCAATATGATCACTT NGG 2 0.14 Tier III
35 NC_000015.10 15 70572255 + LINC02204 NNNAGGCAATATGACCACTG NGG 2 0.1364 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 145520628 - Zfp990 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
2 NC_000070.6 4 145590289 - Gm13212 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
3 NC_000070.6 4 145601387 - Gm13212 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
4 NC_000070.6 4 145679718 - Zfp980 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
5 NC_000070.6 4 146106803 - Zfp987 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
6 NC_000070.6 4 146165362 - Zfp600 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
7 NC_000070.6 4 146518900 - Zfp981 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
8 NC_000070.6 4 146518900 - Zfp993 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
9 NC_000070.6 4 146838421 + Zfp993 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
10 NC_000070.6 4 146899555 + Zfp993 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
11 NC_000070.6 4 146980953 - Rex2 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
12 NC_000070.6 4 146980953 - Zfp993 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
13 NC_000070.6 4 147030369 - Rex2 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
14 NC_000070.6 4 147238472 - Zfp978 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
15 NC_000070.6 4 147315724 - Zfp978 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
16 NC_000070.6 4 147315724 - Zfp988 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
17 NC_000070.6 4 147495818 - Zfp982 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
18 NC_000070.6 4 147562264 - Zfp985 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
19 NC_000070.6 4 147693342 + Zfp534 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
20 NC_000070.6 4 147773012 + Zfp984 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier II
21 NC_000075.6 9 109493820 + Fbxw19 NNNAGACAAAATGAACACTG NGG 2 0.7959 Tier II
22 NC_000069.6 3 159927824 - Wls NNNTGACAATATGAACACTG NGG 2 0.5909 Tier II
23 NC_000068.7 2 12393889 + Mindy3 NNNTGCCAATATAAACACTG NGG 2 0.5874 Tier II
24 NC_000077.6 11 117229356 - Septin9 NNNGGCCAAAATGAACACTG NGG 2 0.5357 Tier II
25 NC_000072.6 6 91393241 + Wnt7a NNNAGAGAATATGAACACTG NGG 2 0.437 Tier II
26 NC_000070.6 4 59717491 + E130308A19Rik NNNAGCCCATATGAACACTG NGG 1 0.4286 Tier II
27 NC_000073.6 7 129372529 - Plpp4 NNNAGCCACTGTGAACACTG NGG 2 0.3714 Tier II
28 NC_000083.6 17 50738122 - Tbc1d5 NNNAGCCAGTAGGAACACTG NGG 2 0.3333 Tier II
29 NC_000071.6 5 67310230 - Slc30a9 NNNTGCCAATATGAACACTC NGG 2 0.2727 Tier II
30 NC_000067.6 1 169526115 - Nuf2 NNNATCTAATATGAACACTG NGG 2 0.2438 Tier II
31 NC_000077.6 11 44835351 - Ebf1 NNNAGCCAAGATCAACACTG NGG 2 0.2105 Tier II
32 NC_000076.6 10 84776646 + Rfx4 NNNTGCCAATTTGAACACTG NGG 2 0.1958 Tier II
33 NC_000070.6 4 15010060 + Necab1 NNNAGCCAATGTTAACACTG NGG 2 0.195 Tier II
34 NC_000073.6 7 140255865 - 5830411N06Rik NNNGGCCAATATGAACACAG NGG 2 0.1786 Tier II
35 NC_000071.6 5 121999750 - Cux2 NNNAGACAATATGAAGACTG NGG 2 0.1429 Tier II
36 NC_000076.6 10 58552167 + Ccdc138 NNNAGCCAATGTGATCACTG NGG 2 0.13 Tier II
37 NC_000079.6 13 10031094 + Chrm3 NNNAGCCAAAATGAACTCTG NGG 2 0.1143 Tier II
38 NC_000072.6 6 53327473 + Creb5 NNNAGGCAATATGAACACTG NTG 2 0.0195 Tier II
39 NC_000073.6 7 68064503 - Igf1r NNNAGCCAACATGAACACTG NGT 2 0.0086 Tier II
40 NC_000070.6 4 146165362 - Vmn2r-ps17 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier III
41 NC_000070.6 4 146518900 - Vmn2r-ps16 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier III
42 NC_000070.6 4 146899555 + Gm21411 NNNAGTCAATATGAACACTA NGG 2 0.8705 Tier III
43 NC_000067.6 1 51227570 + 9330175M20Rik NNNAGGCAAAATGAACACTG NGG 2 0.4286 Tier III
44 NC_000076.6 10 116725350 + 1700030O20Rik NNNAGCCAATATGAACATGG NGG 2 0.1607 Tier III
45 NC_000074.6 8 80582936 - Gm46044 NNNAGCCCATATTAACACTG NGG 2 0.1286 Tier III
46 NC_000070.6 4 120208500 - Gm36134 NNNACCCAATATGAACACTG NGA 2 0.0546 Tier III
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Other clones with same target sequence:

(none)