Construct: sgRNA BRDN0001145792
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGGGATGAACTGCTCCAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CHKA (1119)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76586
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
68074733 |
+ |
CHKA |
NNNGGATGAACTGCTCCAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
6165913 |
- |
ACSBG2 |
NNNCGATGACCTGCTCCAGT |
NGG |
2 |
0.1765 |
Tier I |
| 3 |
NC_000008.11 |
8 |
117115288 |
+ |
SLC30A8 |
NNNGGAGGAACTGCTCTAGT |
NGG |
2 |
0.2745 |
Tier II |
| 4 |
NC_000007.14 |
7 |
81734960 |
- |
HGF |
NNNGGTTGAACTGCTCCAGG |
NGG |
2 |
0.1261 |
Tier II |
| 5 |
NC_000016.10 |
16 |
67521503 |
- |
RIPOR1 |
NNNGGATGAACTGCTCCCCT |
NGG |
2 |
0.0854 |
Tier II |
| 6 |
NC_000003.12 |
3 |
25291528 |
- |
RARB |
NNNGGCTGAACTGCTGCAGT |
NGG |
2 |
0.0699 |
Tier II |
| 7 |
NC_000001.11 |
1 |
212859286 |
+ |
FLVCR1 |
NNNGGATGAACTGCCCCAGG |
NGG |
2 |
0.0481 |
Tier II |
| 8 |
NC_000017.11 |
17 |
66693232 |
- |
PRKCA |
NNNGGATGAACTGCTCTAGC |
NGG |
2 |
0.0424 |
Tier II |
| 9 |
NC_000004.12 |
4 |
185351417 |
- |
SNX25 |
NNNGGATTAACTGCTCCAGT |
NTG |
2 |
0.0244 |
Tier II |
| 10 |
NC_000007.14 |
7 |
153942067 |
+ |
DPP6 |
NNNGGATGAGCTGCTCCAGT |
NTG |
2 |
0.0216 |
Tier II |
| 11 |
NC_000001.11 |
1 |
227935629 |
- |
WNT9A |
NNNGGATGAACTGCTCCAGG |
NGA |
2 |
0.0123 |
Tier II |
| 12 |
NC_000011.10 |
11 |
61416573 |
- |
CPSF7 |
NNNGGATGAACTGGTCCTGT |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000007.14 |
7 |
63987476 |
+ |
LOC107986731 |
NNNGGTTGAACTGCTCCAGA |
NGG |
2 |
0.4018 |
Tier III |
| 14 |
NC_000005.10 |
5 |
161956270 |
- |
LINC01202 |
NNNGGATAACCTGCTCCAGT |
NGG |
2 |
0.3333 |
Tier III |
| 15 |
NC_000008.11 |
8 |
117115288 |
+ |
LOC105375716 |
NNNGGAGGAACTGCTCTAGT |
NGG |
2 |
0.2745 |
Tier III |
| 16 |
NC_000019.10 |
19 |
6165913 |
- |
LOC105372255 |
NNNCGATGACCTGCTCCAGT |
NGG |
2 |
0.1765 |
Tier III |
| 17 |
NC_000022.11 |
22 |
22704246 |
+ |
IGL |
NNNGGATGCACTGCCCCAGT |
NGG |
2 |
0.1558 |
Tier III |
| 18 |
NC_000022.11 |
22 |
22704246 |
+ |
LL22NC03-102D1.18 |
NNNGGATGCACTGCCCCAGT |
NGG |
2 |
0.1558 |
Tier III |
| 19 |
NC_000016.10 |
16 |
67521503 |
- |
LOC100505942 |
NNNGGATGAACTGCTCCCCT |
NGG |
2 |
0.0854 |
Tier III |
| 20 |
NC_000023.11 |
X |
113939326 |
+ |
LOC124905236 |
NNNGGATGAACTGCTCCAGC |
NAG |
2 |
0.0236 |
Tier III |
| 21 |
NC_000001.11 |
1 |
119688957 |
- |
LOC105378937 |
NNNGGATGAACTGGTCCAGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
3884570 |
- |
Chka |
NNNGGATAAACTGCTCCAGT |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
81295727 |
+ |
Pde8a |
NNNTAATGAACTGCTCCAGT |
NGG |
2 |
0.3152 |
Tier I |
| 3 |
NC_000071.6 |
5 |
34639215 |
+ |
Nop14 |
NNNGGGTGAACTGCTTCAGT |
NGG |
2 |
0.2198 |
Tier I |
| 4 |
NC_000073.6 |
7 |
118665431 |
+ |
Tmc5 |
NNNGGAGGAAGTGCTCCAGT |
NGG |
2 |
0.1471 |
Tier II |
| 5 |
NC_000071.6 |
5 |
117980555 |
+ |
Fbxo21 |
NNNGTATGAACTGCTCCACT |
NGG |
2 |
0.1345 |
Tier II |
| 6 |
NC_000067.6 |
1 |
154760899 |
- |
Cacna1e |
NNNGGATGCACTGCTGCAGT |
NGG |
2 |
0.0879 |
Tier II |
| 7 |
NC_000074.6 |
8 |
83035180 |
+ |
Rnf150 |
NNNGGATGAACTTCTCCAGT |
NAG |
2 |
0.0778 |
Tier II |
| 8 |
NC_000069.6 |
3 |
146254128 |
- |
Lpar3 |
NNNGGAGGAACTGCTCCAGC |
NGG |
2 |
0.0535 |
Tier II |
| 9 |
NC_000075.6 |
9 |
123077827 |
- |
Zdhhc3 |
NNNGGATGAACTGGCCCAGT |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000080.6 |
14 |
23059055 |
- |
Gm10248 |
NNNGGATGAACTGCACCAGA |
NGG |
2 |
0.3257 |
Tier III |
Other clones with same target sequence:
(none)
